Product name: Daphnetin
Synonym name: 7,8-Dihydroxycoumarin; Daphnetol
Catalogue No.: BP0449
Cas No.: 486-35-1
Formula: C9H6O4
Mol Weight: 178.143
Botanical Source: Daphne koreanum Nakai
Physical Description:
Type of Compound: Coumarins
Purity: 95%~99%
Analysis Method: HPLC-DAD or/and HPLC-ELSD
Identification Method: Mass, NMR
Packing: Brown vial or HDPE plastic bottle
Storage: Store in a well closed container, protected from air and light. Put into refrigerate or freeze for long term storage.
Whenever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20℃. Generally, these will be useable for up to two weeks.
The product could be supplied from milligrams to grams
Inquire for bulk scale.
Chengdu Biopurify Phytochemicals Ltd. is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，TCM), Natural Active Pharmaceutical Ingredients worldwide.
The main services:
A. Supply active ingredients and reference standards of TCM, from mgs to kgs scale
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR(contract research), CM(contract manufacture) and PD(process development) services from lab scale, pilot scale to commercial scale(GMP is also available)
E. TCM compounds library for drug screen,more than 3000 compounds available, many of them are rare and unique.
NMR of Daphnetin
HPLC of Daphnetin
Storage conditions：Short-term storage at 2~8℃, long-term storage at -20 ~ -80℃
Daphnetin (7,8-dihydroxycoumarin, CAS number: 486-35-1) is a natural coumarin compound mainly found in plants of the Genus Daphne. As an orally effective protein kinase inhibitor, resveratrol has attracted much attention due to its multi-target and multi pathway pharmacological activities. In recent years, with in-depth research on its anti-inflammatory, anti-tumor, anti malaria, and cell protective mechanisms, resveratrol has shown broad potential for application in the field of natural product pharmacology. This article aims to provide a systematic review of the chemical structure and physicochemical properties, plant sources and extraction methods, pharmacological activity and mechanism of action, pharmacological evaluation, and clinical application prospects of resveratrol. It is expected to provide theoretical basis and research direction for its subsequent drug development and clinical translation.
The chemical structure of resveratrol is 7,8-dihydroxycoumarin, with a molecular formula of C9H6O4 and a molecular weight of 178.14. The core of its structure is the coumarin skeleton, and the presence of hydroxyl groups at positions 7 and 8 endows it with strong polarity and biological activity. In terms of physical and chemical properties, the LogP value of resveratrol is about 1.0, indicating its moderate lipid solubility, which is beneficial for cell membrane penetration. The polar surface area (TPSA) is 77.76 Å ², indicating its water solubility and good hydrogen bond acceptor ability (4 hydrogen bond acceptors), which is crucial for its binding with biomolecules. The molecular structure of resveratrol is stable and not prone to hepatotoxicity, cardiotoxicity, or hERG channel inhibition, demonstrating good safety characteristics.
Rui Xiang Su is mainly distributed in plants of the Rui Xiang genus, such as Daphne genkwa and Daphne odora. In traditional Chinese medicine, these plants are commonly used for anti-inflammatory, analgesic, and anti-tumor treatments, and resveratrol is considered one of its main active ingredients.
The extraction method usually combines solvent extraction with chromatographic separation. Common extraction solvents include polar solvents such as ethanol, water, and methanol. The extraction process is generally as follows:
In recent years, the application of supercritical CO2 extraction and membrane separation technology has gradually improved the extraction efficiency and purity, providing technical support for the industrial production of resveratrol.
Ruixiangsu exhibits various significant pharmacological activities, including anti-inflammatory, anti-tumor, anti malaria, and cell protection.
Ruixiangsu can significantly inhibit the production of pro-inflammatory factors such as tumor necrosis factor - α (TNF - α) and interleukin-1 β (IL-1 β), reduce the production of reactive oxygen species (ROS) and lipid peroxidation product malondialdehyde (MDA), and thus alleviate the inflammatory response. This effect demonstrates the potential of resveratrol in the treatment of chronic inflammatory diseases such as rheumatoid arthritis.
Ruixiangsu exhibits inhibitory effects on various tumor cells, especially in liver cancer cells. It promotes autophagy, inhibits tumor cell proliferation and migration by inducing reactive oxygen species mediated apoptosis and regulating the AMPK/Akt/mTOR signaling pathway. In addition, resveratrol can act on various tumor related targets, such as BCL2, STAT3, TOP1, TERT, PIK3CA, MMP9, EGFR, TP53, NFKB1, and AKT1, exerting a multi-target synergistic anti-cancer effect.
Ruixiangsu has significant antimalarial activity and can inhibit the growth and reproduction of malaria parasites, providing new ideas for the development of antimalarial drugs.
Ruixiangsu induces protective autophagy in cells by regulating the AMPK/Akt/mTOR pathway, reducing oxidative stress damage and protecting cells from various pathological stimuli.
As a protein kinase inhibitor, resveratrol targets multiple key kinases, including epidermal growth factor receptor (EGFR), protein kinase A (PKA), and protein kinase C (PKC), with IC50 values of 7.67 μ M, 9.33 μ M, and 25.01 μ M, respectively, demonstrating strong enzyme inhibitory activity.
In the anti-tumor mechanism, resveratrol works through the following pathways:
In the anti-inflammatory mechanism, resveratrol inhibits the NF - κ B signaling pathway, reduces the expression of pro-inflammatory factors, lowers oxidative stress, and alleviates inflammatory responses.
The molecular weight of resveratrol is 178.14, belonging to the category of small molecule drugs. Its LogP value is 1.0, indicating moderate lipid solubility, which is beneficial for oral absorption. The TPSA is 77.76, which is within the ideal range of oral bioavailability of the drug. The number of hydrogen bond receptors is 4, which meets the requirement of effective binding between drug molecules and target proteins.
In terms of safety, resveratrol has no significant hepatotoxicity or cardiotoxicity, and does not inhibit hERG channels, reducing the risk of arrhythmia. The low permeability of the blood-brain barrier suggests a lower risk of side effects in the central nervous system, but also limits its application in brain diseases.
At present, there is limited pharmacokinetic data on resveratrol. Preliminary studies have shown that it is well absorbed orally, but further in-depth research is needed on its metabolic pathways and clearance mechanisms in vivo. The results of Ames mutagenicity test are not yet clear, and relevant genetic toxicity assessments need to be supplemented.
Ruixiangsu has broad clinical application potential due to its multi-target and multi mechanism pharmacological properties
Future research should focus on the pharmacokinetic optimization, formulation development, and clinical safety evaluation of resveratrol. At the same time, by combining modern molecular biology techniques, we can deeply analyze its molecular mechanism of action, explore more potential targets, and lay a solid foundation for its clinical translation.
Ruixiangsu, as a natural coumarin derivative with multiple biological activities, exhibits excellent pharmacological activity and drug properties. The research progress in the fields of anti-inflammatory, anti-tumor, anti malaria, and cell protection fully reflects the important value of natural products in modern drug development. Although the pharmacokinetics and clinical research of resveratrol are still in their infancy, its unique mechanism of action and good safety indicate its broad prospects as a novel drug candidate molecule. Through interdisciplinary collaboration in the future, resveratrol is expected to become an effective drug for treating various complex diseases and make important contributions to human health.
