Product name: Corynoline
Synonym name:
Catalogue No.: BP0398
Cas No.: 18797-79-0
Formula: C21H21NO5
Mol Weight: 367.401
Botanical Source: Corydalis bungeana Turcz.
Physical Description:
Type of Compound: Alkaloids
Purity: 95%~99%
Analysis Method: HPLC-DAD or/and HPLC-ELSD
Identification Method: Mass, NMR
Packing: Brown vial or HDPE plastic bottle
Storage: Store in a well closed container, protected from air and light. Put into refrigerate or freeze for long term storage.
Whenever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20℃. Generally, these will be useable for up to two weeks.
The product could be supplied from milligrams to grams
Inquire for bulk scale.
Description:
Corynoline is a reversible and noncompetitive inhibitor of acetylcholinesterase(AChE) with the IC50 value of 30.6 microM, which exhibits the potent anti-inflammatory and/or immunosuppressive activity, the potent inhibitory activity of corynoline for the ICAM-1/LFA-1 adhesion and would be important on developing the clinically usable drugs for the inflammatory diseases.
References:
Bioorg Med Chem. 2005 Mar 1;13(5):1867-72.
Cell adhesion inhibitory activity of (d)-corynoline, a hexahydrobenzo[c]phenanthridine-type alkaloid, and its structure-activity relationship, studied by X-ray crystal structure analysis and molecular docking study.
Corynoline (1), a hexahydrobenzo[c]phenanthridine-type alkaloid, exhibited the concentration-dependent inhibition for the adhesion of human polymorphonuclear leukocyte and eosinophil to human umbilical vein cultured endothelial cell in the concentration range of showing no significant cytotoxicity for the cell: IC(50) value=72.4 microM for (d)-1 and 156.7 microM for (l)-1. This shows the potent anti-inflammatory and/or immunosuppressive activity of 1.
METHODS AND RESULTS:
To elucidate possible structure-activity relationship, the conformational/structural feature of (d)-1 was investigated by X-ray crystal structure analysis and molecular orbital energy calculations, and the docking study was performed for its interaction with the D1-domain of ICAM-1 (intracellular adhesion molecule-1). A plausible model was proposed, in which all polar atoms of (d)-1 are linked by hydrogen bonds or electrostatic interactions with the functional residues of ICAM-1, that have been supposed to be necessary for the binding with LFA-1 (leukocyte function-associated antigen-1).
CONCLUSIONS:
This suggests the potent inhibitory activity of 1 for the ICAM-1/LFA-1 adhesion and would be important on developing the clinically usable drugs for the inflammatory diseases.
Storage conditions：Short-term storage at 2~8℃, long-term storage at -20 ~ -80℃
Corynoline, CAS number 18797-79-0, is a natural alkaloid derived from the traditional Chinese medicine Corydalis bungeana Turcz. As an orally active acetylcholinesterase (AChE) inhibitor, Zijingling exhibits significant potential in various pharmacological activities such as neuroprotection, anti-inflammatory, anti-tumor, and analgesic effects. In recent years, with the deepening development of natural product pharmacology, Zijingling has gradually become a hot molecule in the research of diseases such as tumors and inflammatory pain due to its multi-target and multi mechanism pharmacological properties. This article aims to provide a systematic review of the chemical structure, plant origin, pharmacological activity, mechanism of action, pharmacological evaluation, and clinical application prospects of Zijingling, providing a theoretical basis and research direction for its subsequent development.
Zijingling is an isoquinoline alkaloid with a molecular formula of C22H23NO5 and a molecular weight of 383.39. Its structural features mainly include a polycyclic isoquinoline skeleton containing multiple hydroxyl and methoxy substituents, which endow it with good hydrophilicity and intermolecular hydrogen bonding ability. The LogP value of purple violet spirit is 2.46, indicating moderate lipid solubility, which is beneficial for its cell membrane penetration and in vivo distribution. Its topological polar surface area (TPSA) is 82.85 Å ² and the number of hydrogen bond receptors is 6, indicating that it has a certain polarity, which may affect its bioavailability and blood-brain barrier penetration ability. The safety indicators of Zijingling, including blood-brain barrier permeability, hepatotoxicity, cardiotoxicity, and hERG channel inhibition, are not yet clear and require further systematic evaluation.
The chemical structural formula of purple violet spirit is as follows (schematic):
[异喹啉核心结构]
- 多羟基和甲氧基取代
- 分子量：383.39
- LogP：2.46
- TPSA：82.85
- 氢键受体数：6
The complexity of its structure and diverse functional groups provide a molecular basis for its multi-target effects.
Purple violet spirit mainly exists in plants of the purple violet genus, especially in Corydalis bungeana and related species. Purple violet is a plant of the Ranunculaceae family, widely distributed in northern and northeastern China. It is commonly used in traditional Chinese medicine for clearing heat, detoxifying, reducing swelling, and relieving pain.
The extraction of purple violet spirit usually involves the following steps:
In recent years, new technologies such as ultrasound assisted extraction and microwave-assisted extraction have also been applied to the extraction of purple violet spirit, significantly improving the extraction efficiency and purity.
Zijingling, as a multifunctional natural product, has shown significant biological activity in various pharmacological models, mainly including:
The inhibitory effect of Zijingling on AChE is the basis of its neuroprotective effect. In vitro experiments showed that the AChE inhibitory IC50 of purple violet spirit was approximately 30.6 μ M, indicating its moderate inhibitory activity. AChE inhibitors have significant value in the treatment of neurodegenerative diseases such as Alzheimer's disease, and therefore Zijingling is considered a potential neuroprotective agent.
Zijingling has shown good anti-inflammatory effects in various inflammatory models. The mechanism mainly reduces the inflammatory response by inhibiting the expression of inflammatory factors such as TNF - α, IL-1 β, and IL-6. In vivo experiments have shown that Zijingling can significantly reduce pain behavior in inflammatory pain models, indicating its potential in the treatment of inflammatory pain.
Zijingling exhibits inhibitory effects on proliferation and induces apoptosis in various tumor cell lines such as lung cancer. Research has shown that Zijingling can regulate various tumor related signaling pathways, including BCL2, STAT3, RELA (NF - κ B subunit), MAPK family members (MAPK1, MAPK8), and PI3K/AKT pathway, exerting anti-tumor effects. In addition, the mechanism by which purple violet spirit induces apoptosis in tumor cells is closely related to the activation of apoptosis related enzymes such as CASP9.
Zijingling has shown analgesic effects in both neuropathic pain and inflammatory pain models. The analgesic mechanism may be related to inhibiting the release of inflammatory mediators and regulating neurotransmitters, and the specific mechanism still needs further clarification.
The multi-target mechanism of action of Zijingling is the molecular basis for its multiple pharmacological activities. Through molecular docking, cell experiments, and animal model studies, Zijingling mainly acts on the following targets:
In summary, the Purple Osmanthus Lingtong exhibits a wide range of pharmacological effects through the synergistic regulation of multiple targets and pathways.
The pharmacological parameters of Zijingling indicate that it has certain potential for drug development:
However, the key pharmacokinetic and toxicological indicators such as blood-brain barrier permeability, safety (hepatotoxicity, cardiotoxicity, hERG channel inhibition), and genotoxicity (Ames test) of Zijingling are currently unclear, which limits its preclinical development. In the future, it is necessary to comprehensively evaluate its safety and efficacy through in vivo pharmacokinetic studies (such as absorption, distribution, metabolism, excretion of ADME) and systematic toxicology evaluations.
Purple violet spirit, as a multifunctional natural product, has a wide range of clinical application potential:
Future research should focus on the pharmacokinetic optimization, structural modification to enhance activity and safety, as well as the development of preclinical animal models and early clinical trials of Corydalis purpurea. At the same time, combining modern molecular biology and medicinal chemistry techniques, we will deeply analyze its mechanism of action and promote the clinical translation of Zijingling.
Zijingling, as a natural product with multiple pharmacological activities, has shown extensive potential for applications in neuroprotection, anti-inflammatory, anti-tumor, and analgesic fields. Its unique chemical structure and multi-target mechanism of action provide valuable molecular templates for the development of natural product drugs. Although the safety and pharmacokinetic data of Zijingling are not yet complete, its value as a new drug candidate molecule is increasingly prominent with the deepening of research. In the future, through systematic pharmacological research and preclinical evaluation, Zijingling is expected to become a new hope for the treatment of various diseases and promote the innovative development of natural product pharmacology.