1、 Pharmacopoeia standard for blood interruption
This product is the dry aboveground part of Labiatae plant Clinopodium polyphalum (Vaniot) c.y.wu et Hsuan or Clinopodium chinense (benth.) 0.kuntze. Harvest before flowering in summer, remove sediment and dry in the sun.
[character] the stem of this product is square columnar, concave and grooved on all sides, with opposite branches, 30 ~ 90cm long and 1.5 ~ 4mm in diameter; the upper part is densely covered with gray white hairs, the lower part is sparse or nearly hairless, the internode is 2 ~ 8cm long, and the surface is grayish green or greenish brown; It is brittle, easy to break, with uneven section and medullary or hollow center. The leaves are opposite and stippled, and the leaves are mostly shrunk and broken. The intact ones are oval after flattening, with a length of 2-5cm and a width of 1.5-3.2cm; the edges are sparsely serrated, the upper surface is greenish brown, the lower surface is grayish green, and both sides are densely covered with white hairs. .
[identification] (1) the leaf surface of this product: the vertical circumferential wall of the lower epidermal cells is wavy, and the stomata are straight axis. The non glandular hairs are slender and numerous, consisting of 1-9 cells, reaching 1440 μ m in length, and some basal cells are expanded, reaching 102 μ m in diameter; The diameter of the middle cell is 10-55 μ m, and some cells are constricted with warty protrusions on the surface. The glandular scale head is mostly 8-cell, with a diameter of 60 μ m, and the stalk is single-cell and extremely short. The head and stalk of the glandular hair are single-cell, and the head diameter is about 20 μ M.
(2) Take 1g of the product powder, add 10ml of methanol, heat and reflux for 30 minutes, filter, evaporate the filtrate, add 10ml of water to dissolve the residue, add 10ml of diethyl ether to shake and extract twice, 10ml each time, discard the ether solution, add water to saturate the water with n-butanol to shake and extract twice, 10ml each time, combine the n-butanol solution, evaporate to dryness, add 1ml of methanol to dissolve the residue, place it on a neutral alumina column (100-120 mesh, 5g, inner diameter of 1-1.5cm, wet packed with water), elute with 40ml of 40% methanol, collect the eluent, evaporate to dryness, and add 1ml of methanol to dissolve the residue as the test solution. In addition, take the reference substance of buddleoside Ⅳ B and add methanol to make a solution containing 2mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 4 μ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use chloroform methanol glacial acetic acid water (7:2.5:1:0.5) as the developing agent, develop, take out, dry, spray 10% sulfuric acid ethanol solution, heat at 110 ℃ until the spots are clear, and inspect them under sunlight and ultraviolet light (365nm). In the chromatogram of the test sample, the same brownish red spots or brownish red fluorescent spots appear at the corresponding positions of the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 10.0% (the second method of general rule 0832).
The total ash content shall not exceed 10.0% (general rule 2302).
[extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), 75% ethanol shall be used as solvent, which shall not be less than 10.0%.
Decoction pieces
[processing] remove impurities, spray water, moisten slightly, cut into sections, and dry.
This product has irregular sections. The stem is square columnar, concave and grooved on all sides, with grayish green or greenish brown surface, and some are covered with grayish white hairs. The central section is medullary or hollow. The leaves are mostly shrunken and broken, and the intact ones are oval after flattening, with sparse serrations on the edge. The upper surface is greenish brown, the lower surface is grayish green, and both sides are densely covered with white hairs. .
[identification], [inspection], [extract] are the same as that of medicinal materials.
[nature, taste and meridian tropism] slightly bitter, astringent and cool. .
[function and indications] convergence and hemostasis. .
[usage and dosage] 9 ～ 15g. Apply appropriate amount externally, and apply on the affected part at the end of grinding.
[storage] keep dry and damp proof.

2、 Chemical composition of devascularized blood

The whole herb contains clinopodiide A and taraxer-9,12,17-triene-3 β, 23 Di ol, etc.

3、 Pharmacological effects of blood interruption

1. hemostatic effect 1g or 0.5g of blood cutting pollen applied to the incision of carotid artery, femoral artery, liver and hindlimb skin and muscle cutting wounds of rabbits can significantly shorten the hemostatic time, and its effect is stronger than that of Yunnan Baiyao. . Blood breaking fluid extract, alcohol extract and crude saponin can increase the contraction of isolated vascular strips of rabbits and guinea pigs; Compared with norepinephrine, its action is slow, mild and long-lasting. Alcohol extract had the strongest effect; It has the most obvious effect on uterine artery strips. This effect is caused by the direct excitation of vascular smooth muscle. 20mg of alcohol extract, 20mg of water extract, 12mg of crude saponin or 20% aqueous solution of alcohol extract could significantly reduce the vascular perfusion volume of isolated rabbit ears. The alcohol extract 0.8% aqueous solution significantly increased the renal elbow perfusion pressure in rats in vivo. . Water extract and 0.2% crude saponin aqueous solution significantly reduced the vascular perfusion volume of toads' hind limbs. When 10% of the infusion was added to the frog mesentery, arterial contraction and venous dilatation could be seen. The crude saponin of devascularization was subcutaneously injected into mice at a dose equivalent to 2mg (crude drug) / 10g, which significantly inhibited the increase in capillary permeability induced by histamine squamate. It can significantly inhibit the increase of capillary permeability caused by histamine phosphate. 10 μ l of 0.5% total saponins from devascularized rats significantly increased ADP induced platelet aggregation in vitro. Administration of 2% total saponins at 160mg/kg to mice for 4 days significantly enhanced ADP induced platelet aggregation, and the effect was stronger than that of the same genus of C. chinensis. .

2. inhibit immune function and cut off blood flow total saponin 75mg/kg was injected intraperitoneally into mice for 6 consecutive days. It was found that the high-dose group had an inhibitory trend on the weight of thymus and spleen; Significantly inhibit the clearance of carbon particles; The serum IgG content of mice was significantly increased, and the same dose, administered for 3D, could inhibit the phagocytosis of peritoneal macrophages in mice; .

3. anti inflammatory and bacteriostatic effects total saponins of devascularization can inhibit carrageenan induced rat foot swelling, resist histamine induced increase in skin capillary permeability in mice, and excite adrenal cortex function. The warm extract has bactericidal effect on Staphylococcus aureus, Pseudomonas aeruginosa and dysentery in the test tube. The minimum inhibitory concentrations of the alcohol extract against Staphylococcus aureus, Streptococcus pneumoniae and Escherichia coli were 1:10, 1:20 and 1:10, respectively.