1、 No pharmacopoeia standard in summer
This product is the dried tuber of Corydalis decumbens (thunb.) pers., a plant of Papaver family. After emergence in spring or early summer, the seedlings shall be dug, and the stems, leaves and fibrous roots shall be removed, washed and dried.
[properties] this product is quasi spherical, oblong or irregular block, 0.5 ~ 3cm long and 0.5 ~ 2.5cm in diameter. The surface is grayish yellow, dark green or dark brown, with tumor like protrusions and inconspicuous fine wrinkles, the top is blunt, stem marks can be seen, and there are light yellow punctate leaf marks and fibrous root marks around. . The breath is slight and the taste is bitter.
[identification] (1) the powder of this product is light yellowish brown. The thick walled cells of the lower epidermis are patchy, yellowish brown, oblong like or irregular in shape, with slightly thick walls and intermittent beads, often with mural pores. Parenchyma cells are yellowish or almost colorless, square like or round like; The threaded conduit or reticulated conduit is small. The single starch granule is round or oblong, with a diameter of 5-16 μ m, umbilical punctate or bird shaped, and the compound granule is composed of 2-6 grains. Gelatinized starch granules are faintly visible, or traces of gelatinized starch granules can be seen after permeabilization with chloral hydrate.
(2) Take 4G of crude powder of this product, add 25ml of 1% sodium carbonate solution, soak it in a near boiling water bath for 5 minutes, filter it, adjust the pH value of the filtrate to 6 with dilute hydrochloric acid, add 15ml of trichloromethane, shake it for extraction, take 2ml of trichloromethane solution, add 1ml of sulfuric acid, shake it, the sulfuric acid layer will turn brown red, and turn brown black after placing.
(3) Take 4G of this product powder, add 40ml of mixed solution of trichloromethane methanol concentrated ammonia test solution (5:1:0.1), sonicate for 30 minutes, filter, concentrate the filtrate to dryness, add 2ml of methanol to dissolve the residue as the test solution. In addition, take the reference substance of protoopioid base and add chloroform to make a solution containing 2mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 5 μ l each of the above two solutions, dot them on the same silica gel G thin-layer plate, pre saturate for 15 minutes with cyclohexane ethyl acetate diethylamine (16:3:1) as the developing agent, develop, take out, air dry, and spray with dilute bismuth potassium iodide test solution. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
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[extract] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 8.0%.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Acetonitrile triethylamine acetic acid solution (8ml triethylamine, 30ml glacial acetic acid, diluted to 1000ml with water) (18:82) was used as the mobile phase; The detection wavelength of protoopioid and palmatine hydrochloride was 289nm and 345nm, respectively. The number of theoretical plates should not be less than 3000 according to the peak of protoopioid and palmatine hydrochloride.
Preparation of reference solution take 10mg of protoopioid reference, weigh accurately, put into a 50ml volumetric flask, add 5ml of 1% hydrochloric acid solution to dissolve, add methanol to the scale, and shake well. Take 10mg of bamatine hydrochloride reference substance, weigh accurately, put it into a 100ml volumetric flask, add methanol to dissolve and dilute to the scale, and shake well. Accurately measure 5ml of each of the above two solutions, put them into the same 25ml measuring flask, add methanol to the scale, and shake well to obtain (each 1ml contains 40 μ g of protoopioid base and 20 μ g of palmatine hydrochloride).
Preparation of the test solution take about 0.5g of the fine powder of the product, accurately weigh it, place it in a conical flask with a stopper, precisely add 50ml of methanol, weigh it, heat and reflux for 1 hour, cool it, then weigh it, make up the lost weight with methanol, shake it well, filter it, and take the filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of protoopioid (c20h19no5) shall not be less than 0.30%, and that of bamatine hydrochloride (c21h21no4 • HC1) shall not be less than 0.080%.
[nature, taste and meridian tropism] bitter, pungent and warm. .
[functions and indications] it can activate blood circulation and relieve pain, relax muscles and activate collaterals, dispel wind and remove dampness. .
[usage and dosage] 6-12g. Take it three times at the end of the study.
[storage] store in a ventilated and dry place.

2、 No chemical composition in summer

Tuber containsAstaxanthine, violamidine,Bicucuoling，Palmatine， α - allocryptopine,berberine，Jatrorrhizine， A-tetrahydropalmatine, haloxyphylline,Protoopioid, mesalazine, amoxicilline, epi-a-amoxicilline, hydroxypalmatine,Corydalis aandSummer soda CEt al.

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. It can also resist the tension of cerebral blood vessels and lower limb blood vessels caused by norepinephrine. The drug cannot relieve the cerebral vascular tension produced by serotonin. Its vasodilatory effect on the brain was not negated by atropine. Both in vitro and in vivo administration of total alkaloids of Rhizoma Corydalis significantly inhibited ADP induced platelet aggregation in rats, and significantly inhibited thrombosis and platelet adhesion. Intravenous injection of 0.3mg/kg significantly inhibited experimental thrombosis in rats, with an inhibition rate of 31.3%. It is suggested that the above effects may be one of the effective mechanisms of the drug in the treatment of cerebrovascular embolism and other diseases. Summer alkaloid free solution can prolong the blood clotting time of mice; Increase coronary blood flow and hypotensive effect in dogs. In addition, it has been proved that it can stimulate the isolated intestine and uterus of rats; It has no blocking effect on the excitation transmission of cardiac vagal ganglion and cervical sympathetic ganglion in dogs. The physiological characteristics of isolated papillary and atrial muscles of guinea pigs were observed. The results showed that 10mg/l could significantly prolong the functional refractory period and inhibit the adrenal induced automaticity; But it does not reduce excitability and has no obvious effect on contractility. It is suggested that summer has no anti arrhythmic effect. Xia Xia Wu injection can reduce the hindlimb vascular resistance of anesthetized dogs. After injection of 100 μ g / kg of Xia Xia Wu injection, the blood pressure decreased from 124.4 ± 5.2mmhg to 111.6 ± 4.8mmhg; . After 10 μ g/kg of xiayanwu injection via femoral artery, the blood pressure did not change, but the hindlimb vascular resistance index decreased by 56.8 ± 4.8. . The experiment also showed that summer has no dilatory effect on the peripheral blood vessels of anesthetized dogs, not through the excitation of adrenergic b-receptors, nor through the blockade of adrenergic a receptors, and has nothing to do with histamine receptors, but is related to the excitation of cholinergic receptors.

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