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. It can be dug all year round to remove sediment, dried or burned to remove fluff (scales).
[properties] the product is flat, long strip, curved, branched, 5-15cm long, 1-1.5cm wide, and 0.2-0.5cm thick. The surface is densely covered with dark brown to dark brown small scales, soft as hair. After burning, it is brown or dark brown, with protruding or concave circular leaf marks on both sides and upper surface, and a few petiole residues and fibrous roots remain. It is light, brittle, and easy to break. Its cross section is reddish brown. The vascular bundles are yellow punctate and arranged in rings. It is light and slightly astringent.
. The base of the scale is attached to the depression of the epidermis and consists of 3-4 rows of cells; It contains brownish red pigment. The vascular bundle is peripherally tough, with 17-28 arranged in rings; There is an endothelial layer around each vascular bundle, and the Kjeldahl point can be seen; Xylem tracheids are polygonal.
Powder tan. The scale fragments are brownish yellow or brownish red, and the body cells are long strip or irregular, with a diameter of 13-86 μ M. the wall is slightly curved or straight, and there are often hairs on the edge. The two cells coexist and the apex is separated; The cells in the stalk were irregular in shape. The basic tissue cells were microlignified, and the pore groove was obvious, with a diameter of 37 ～ 101 μ M.
(2) Take 0.5g of this product powder, add 30ml of methanol, heat and reflux for 1 hour, cool, filter, evaporate the filtrate, add 1ml of methanol to dissolve the residue, and use it as the test solution. In addition, take naringin reference substance and add methanol to make a solution containing 0.5mg per 1ml as the reference substance solution. Test according to thin-layer chromatography (general rule 0502), suck 4 μ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use the upper solution of toluene ethyl acetate formic acid water (1:12:2.5:3) as the developing agent, develop, take out, dry, spray aluminum trichloride test solution, and inspect under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions of the chromatogram of the control sample.
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[effluent] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), using dilute ethanol as solvent, it shall not be less than 16.0%.
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Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol acetic acid water (35:4:65); The detection wavelength was 283nm. The number of theoretical plates should not be less than 3000 according to the naringin peak.
Preparation of reference solution take an appropriate amount of naringin reference, weigh accurately, add methanol to make a solution containing 60 μ g naringin per 1ml.
Preparation of test solution take about 0.25g of crude powder of the product, weigh it accurately, put it into a conical flask, add 30ml of methanol, heat and reflux for 3 hours, cool it, filter it, put the filtrate into a 50ml volumetric flask, wash the container with a small amount of methanol for several times, filter the washing solution into the same volumetric flask, add methanol to the scale, shake it evenly, and get it.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
The content of naringin (c27h32o14) in this product shall not be less than 0.50% according to the calculation of dry product.
Decoction pieces
[processing] Rhizoma Drynariae remove impurities, wash, moisten thoroughly, cut into thick pieces, and dry.
This product is irregular thick film. The surface is dark brown to brownish brown, often with small brown scales remaining, and some circular leaf marks can be seen. The red brown and yellow vascular bundles on the section are arranged in a ring in a dot shape. It is light and slightly astringent.
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The total ash content is the same as that of medicinal materials, and shall not exceed 7.0%.
[identification], [extract], [content determination] are the same as those of medicinal materials.
Scald the broken bones and patches, remove the broken bones and patches, scald them with sand until they bulge according to the scalding method (general rule 0213), and remove the hairs.
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[nature, taste and meridian tropism] bitter, warm. Return to liver and kidney channels.
[functions and indications] it can heal and relieve pain, tonify kidney and strengthen bone; For external use, it can eliminate wind and freckle. It is used for tumbling, muscle and bone fracture, kidney deficiency and low back pain, muscle and bone flaccidity, tinnitus and deafness, and loose teeth; .
[usage and dosage] 3 ～ 9g.
[storage] put it in a dry place.

2、 Chemical constituents of Rhizoma Drynariae

Quercus fern rhizome containsNaringin, 21 HEPTENE, 9 (11) amniodene], 7-amniodene, 3-echelon,β - sitosterol,Stigmasterol,CampesterolAnd tetracyclic triterpenoids: cyclocaryophyll sterol acetate, cyclocaryophyll sterol acetate, cyclocaryophyll sterol acetate, 9,10-cyclocaryophyll-25-enol-3 β - acetate. The rhizome of Alpinia oxyphylla contains 21 hepapene, 13 (18) neohepapene, 9 (11) - morbidiene,β - sitosterol，Stigmasterol，Campesterol.

3、 Pharmacological effects of Rhizoma Drynariae

1. according to the research of Zhou tongshui of China Pharmaceutical University, the oral administration of Quercus fern rhizome Decoction (20g/kg, 30g/kg) and naringin (equivalent to 20g/kg of technical drug) can promote the healing of experimental bone injury in rats.

2. gushanbu Decoction 7.5-50g/kg by gavage can stimulate the compensatory proliferation of osteoarticular chondrocytes in rats with experimental arthritis, and can partially improve the degeneration of articular cartilage caused by the change of mechanical stress line, thus reducing the rate of bone and joint lesions.

3. Rhizoma Drynariae dihydroflavonoid glycosides can increase the beating frequency of cultured neonatal rat cardiomyocytes in vitro, make the contraction powerful, and have a pacemaker effect on cardiomyocytes. Its mechanism of action may be similar to a β - receptor agonist.

4. Gu Sui Bu Decoction (100%) 0.8ml/kg orally can significantly prevent the rise of serum cholesterol and triglyceride in experimental hyperlipidemic rabbits, and prevent the formation of atherosclerotic plaque in the aortic wall.

5. the guinea pig experiment suggested that the combination of Drynaria Decoction and kanamycin could reduce the toxic effect of Kanamycin on the cochlea, but could not control the development of toxic deafness after drug withdrawal.

6. Drynaria decoction can inhibit the growth of Staphylococcus in the test tube.

. Gushanbu Decoction 100g (crude drug) /kg/ day was orally administered 2 hours before kanamycin Administration for 8 consecutive days, which could reduce the toxicity of kanamycin to the cochlea, but could not control the development of toxic deafness after drug withdrawal.