1、 Pharmacopoeia standard of Angelica pubescens
This product is the dried root of Angelica pubescens maxim.f.biserrata Shan et yuan, an Umbelliferae plant. When the seedlings just germinate in early spring or the stems and leaves wither in late autumn, excavate them, remove fibrous roots and sediment, bake them until they are semi dry, pile them up for 2-3 days, and then bake them until they are completely dry after softening.
[character] the root of this product is slightly cylindrical, with 2-3 branches or more at the lower part, and is 10-30cm long. The root head is swollen, conical, and many transverse wrinkles, with a diameter of 1.5-3cm, and there are residues or depressions of stems and leaves at the top. The surface is grayish brown or tan, with longitudinal wrinkles, transverse lenticel like protrusions and slightly raised fine root marks. The texture is hard, and becomes soft when affected with moisture. The skin of the section is gray white, with most scattered brown oil chambers, and the wood is gray yellow to yellowish brown, forming a brown ring. It has a special aroma, bitter, pungent and slightly numb tongue.
[identification] (1) cross section of this product: cork cell sequence. The inner layer of the plug is narrow and there are a few oil chambers. ; There are many oil chambers, arranged in several rounds, with a tangential diameter of about 153 μ m and 6-10 surrounding secretory cells. The cambium forms a ring. Xylem ray width 1-2 rows of cells; The ducts are rare, with a diameter of about 84 μ m, and are often arranged in a single radial direction. Parenchyma cells contain starch granules.
(2) Take 1g of this product powder, add 10ml of methanol, sonicate for 15 minutes, filter, and take the filtrate as the test solution. Another 1g of Angelica pubescens control medicinal material was taken, and the control medicinal material solution was prepared by the same method. . Test according to thin-layer chromatography (general rule 0502), suck 8 μ l of the test solution, 4 μ l of the control solution and 4 μ l of the control solution, respectively, onto the same silica gel G thin-layer plate, use petroleum ether (60 ～ 90 ℃) - ethyl acetate (7:3) as the developing agent, develop, take out, dry, and view under ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 10.0% (the fourth method of general rule 0832).
The total ash content shall not exceed 8.0% (general rule 2203).
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[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Acetonitrile water (49:51) was used as mobile phase; .
Preparation of reference solution take an appropriate amount of osthole reference substance and dihydrocarvacrol angelic acid ester reference substance, weigh accurately, add methanol to make solutions containing 150 μ g and 50 μ g per 1ml respectively.
Preparation of test solution take about 0.5g of powder (passing through No. 3 screen), weigh accurately, place in a stoppered conical flask, add 20ml of methanol precisely, close the stopper, weigh, sonicate (power 250W, frequency 40KHz) for 30 minutes, cool, weigh again, make up the lost weight with methanol, shake well, filter, accurately measure 5ml of continuous filtrate, place in a 20ml measuring flask, add methanol to the scale, shake well, filter, and take the continuous filtrate.
The determination method is to precisely suck 10 μ l of the two reference solutions and 10 ～ 20 μ l of the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of osthole (c15h16o3) shall not be less than 0.50%, and the content of dihydrocarvacrol angelic acid ester (c19h20o5) shall not be less than 0.080%.
Decoction pieces
[processing] remove impurities, wash, moisten, slice, dry in the sun or at low temperature.
This product is shaped like a round thin sheet. . The cut skin is grayish white to grayish brown, with most scattered brown oil spots, and the wood is grayish yellow to yellowish brown, forming a brown ring. It has a special aroma. It tastes bitter, pungent and slightly numb.
[inspection] the acid insoluble ash content is the same as that of medicinal materials, and shall not exceed 2.0%.
[identification], [inspection] (moisture and total ash) [content determination] is the same as that of medicinal materials.
[nature, taste and meridian tropism] pungent, bitter and mild. Return to kidney and bladder meridian.
[functions and indications] dispel wind, remove dampness, relieve pain. It is used for wind cold and dampness arthralgia, waist and knee pain, Shaoyin and wind headache, and wind cold and dampness headache.
[usage and dosage] 3 ～ 10g.
[storage] put it in a dry place to prevent mold and moth.

2、 Chemical constituents of Angelica pubescens

The root of Angelica pubescens contains angelic alcoholAngelica sinensis、Bergamot lactone, carvacrol methyl ether, umbelliferolideScopoletin Pavilion、Angelic acid, crotonic acid, palmitic acid, stearic acid, oleic acid, linolenic acid, phytosterols, glucose and a small amount of volatile oil.
Angelica pubescens root contains angelicinIsoanisolactone、Bergamot lactone、Zanthoxylin、Saposhnikol、Isobergamot lactoneAnd other furan coumarins. In addition to the above ingredients, the leaves also contain psoralen, among which the main furanocoumarins areSaposhnikolIt also contains 0.26 ～ 0.57% volatile sleeve.

3、 Pharmacological action of Angelica pubescens

1 Effects on platelet aggregation and experimental thrombosis: alcohol extract of Angelica pubescens (h6f4) (1g extract is equivalent to 19.6g crude drug) can inhibit ADP induced platelet aggregation in rats in vitro, and the inhibition rate of aggregation increases with the increase of drug concentration. . At 1.0g/kg, the inhibition rate could reach 51.1%. H6f4 can also inhibit the in vitro thrombus formed by Chandler method, which not only delays the occurrence time of snowstorm, CTFT and TFT, but also shortens the length of wet thrombus and reduces the wet weight. At the same time, it can significantly prolong the tail bleeding time of mice. Dihydrocarvacrol, dihydrocarvacrol acetate, dihydrocarvacrol, methoxycarvacrol, dihydrocarvacrol glucoside and other related components in Radix Angelicae Pubescentis also had significant inhibitory activity on ADP induced rat platelet aggregation in vitro. At a final concentration of 1mg/ml, the inhibitory rates were 42.2 ± 11.3%, 46.6 ± 2.2%, 24.5 ± 11.3%, 50.4 ± 49.5% and 32.7 ± 9.0%, respectively PAF、 Collagen ionophore A23187 and thrombin induced platelet aggregation have inhibitory effects.

2 Effect on cardiovascular system: the dichloromethane extract of Angelica pubescens mainly contains coumarin compounds such as methoxyapigenin and has the activity of antagonizing calcium channel blocker receptors, which is related to blood pressure and heart rhythm. . It is also reported that the crude preparation of Angelica pubescens, 0.1-0.5ml/kg intravenously, has hypotensive effect on anesthetized dogs and cats. Angelica pubescens also acts on calcium channel blocker receptors.

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4 Analgesic, sedative and anti-inflammatory effects: the intraperitoneal injection of Angelica pubescens Decoction 2g/kg can significantly prolong the animal pain reaction time caused by hot plate method in mice, indicating that Angelica pubescens has obvious analgesic effect. . Duhuo infusion subcutaneously injected into frogs can also prevent convulsions caused by strychnine, but frogs eventually die.

5 Antibacterial effect: apigenin methyl ether and zanthoxylin have a broad antibacterial spectrum against 11 strains in vitro. Florolide has obvious inhibitory effect on Brucella, and its MIC is 1:2500.

6 Photosensitivity: coumarin furans, such as citrus lactone, zanthoxylin and isoimperatorin, are photoactive substances. Once they enter the body and are exposed to sunlight or ultraviolet light, they can cause solar dermatitis on the exposed skin, causing redness, increased pigment, and even epidermal thickening on the exposed part.

7 Other effects: Citrus lactone has moderate protective effect on experimental gastric ulcer. .