1、 Pharmacopoeia standard of Lamiophlomis
This product is a traditional Tibetan medicine. It is the dry aboveground part of Lamiophlomis rotata (benth.) Kudo, a Labiatae plant. Harvest in autumn, wash and dry.
[character] the leaves of this product are rosette shaped, cross opposite, curled, flattened and fan-shaped or triangular oval, 4-12cm long and 5-15cm wide; the apex is blunt or round, the base is shallow cardioid or downward extended into a wide wedge, and the edge has round teeth; The upper surface is greenish brown, and the lower surface is grayish green; Veins fan-shaped, venules reticulate, protuberant; . The inflorescence is slightly tower shaped or short cone-shaped, 3-6cm long; the persistent calyx is brown, tubular bell shaped, with 5-Sided lines, calyx teeth 5, and the apex has long thorny tips. Nutlets obovate triangular. The breath is slight, and the taste is slightly astringent and bitter.
[identification] (1) the powder of this product is brown. Non glandular hairs are numerous, composed of 2-3 cells, with a diameter of 10-15 μ m, thick walls and warty protrusions. . Air hole straight axis type or inequality. The fibers are long fusiform, and the mural holes are transversely split.
(2) Take 1g of this product powder, add 10ml of ethanol, heat and reflux for 15 minutes, filter, and take the filtrate as the test solution. Another 1g of Lamiophlomis lamiophylla reference medicinal material was prepared into the reference medicinal material solution by the same method. Then take the standard sample of sennoside methyl ester and the standard sample of 8-o-acetyl sennoside methyl ester, add ethanol to make a mixed solution containing 0.5mg per 1ml as the control solution. According to the test of thin-layer chromatography (general rule 0502), suck 5-10 μ l of the test solution, 5 μ l of the control medicinal material solution and 5 μ l of the control solution, respectively, and dot them on the same silica gel G thin-layer plate, develop them with chloroform methanol (4:1), take them out, dry them, spray phosphomolybdic acid test solution, heat them at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding positions of the chromatogram of the control medicinal material and the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 13.0% (the second method of general rule 0832).
The total ash content shall not exceed 13.0% (general rule 2302).
Acid insoluble ash content shall not exceed 4.0% (general rule 2302).
.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; With acetonitrile as mobile phase A and water as mobile phase B, perform gradient elution according to the following table; The detection wavelength was 235nm. The number of theoretical plates should not be less than 3000 according to the peak of Geniposide methyl ester.
--------------------------------------------------------
Time (min) mobile phase a (%) mobile phase B (%)

0～11 9 91

11～35 9→18 91→82

35～45 18 82
---------------------------------------------------------
Preparation of reference solution take appropriate amount of Geniposide methyl ester reference and 8-o-acetyl geniposide methyl ester reference, weigh accurately, add methanol to make a mixed solution containing 30 μ g per 1ml.
Preparation of test solution take about 0.6g of powder (passing through No. 3 screen), weigh accurately, place in a corked conical flask, add 25ml of 70% methanol precisely, close the stopper, weigh, heat and reflux for 1 hour, cool, weigh again, make up the lost weight with 70% methanol, shake well, filter, accurately measure 2ml of the continued filtrate, place in a 10ml measuring flask, add methanol to the scale, shake well, filter, and take the continued filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
The total amount of Geniposide methyl ester (c17h26o11) and 8-o-acetyl geniposide methyl ester (c19h28o12) in this product shall not be less than 0.50% according to the dry product.
Decoction pieces
[processing] remove impurities and cut them into pieces.
[identification], [inspection], [content determination] are the same as those of medicinal materials.
[nature, taste and meridian tropism] sweet, bitter and flat. .
[functions and indications] activating blood circulation and hemostasis, dispelling wind and relieving pain. It is used for traumatic injuries, trauma bleeding, rheumatism and arthralgia, and yellow water disease.
[usage and dosage] 2 ～ 3G.
[storage] store in a ventilated and dry place.

2、

cycasin Luteolin（luteolin)， Luteolin-7-o-glucoside,quercetin .
,β - sitosterol（β-sitosterol)，palmitic acid（palmitic acid)， Laminin aLamininB、LamininC et al.
Above ground part includingShanzhiside methyl ester （shanzhiside methyl ester)，8-o-acetyl geniposide methyl ester（8-O-acetylshanzhiside methyl ester)，Linseed glycoside（sesamoside)。

3、 Pharmacological effects of Lamiophlomis rotata

1. effects on immune function the saponins of Lamiophlomis pubescens 500mg/ (kg · d) and the ethanol extract of Lamiophlomis pubescens 300mg/ (kg, d) or 150mg- (kg,) were injected intraperitoneally into tumor bearing mice for 10 days, which had a significant inhibitory effect on transplanted tumor EC, and the saponins also had a significant inhibitory effect on hep solid tumor analysis. . Saponin can also make tumor bearing mice spleen glycoside 50mg/ (kg, d), 100mg/ (kg, d), continuous intraperitoneal injection of animals for 5 days, all significantly improve the phagocytosis rate and index of macrophages, E-rosette formation rate and esterase staining positive rate, indicating that Lamiophlomis has the effect of significantly improving non-specific immunity and specific cellular immunity.

2. analgesic effect when 10% of the extract of Lamiophlomis pubescens was given to mice by gavage at 0.28ml/10g and 0.14ml/10g, it had obvious analgesic effect, lasting for about 2H. The extract could also significantly inhibit the acetic acid writhing reaction in mice, and the analgesic effect of 0.19-3.0g/kg was equivalent to that of 0.23g/kg aspirin.

3. hemostatic effect in the mouse tail vein hemostasis experiment, 0.14ml/10g of 10% and 5% extract were given to the mouse thin stomach, which had significant hemostatic effect. Lamiophlomis pubescens has local hemostatic effect on rat liver cutting wound.

4. antibacterial effect the extract of Lamiophlomis rotata has antibacterial effect on B hemolytic streptococcus and aerogenic bacillus in the filter paper method. .

. The extract 1.5g/kg was injected into the intestine of anesthetized rabbits, and there was no poisoning. When the extract was administered to dogs at 0.1g/kg and 0.5g/kg for 21 consecutive days, it had no obvious effect on animal activity, general state, hemogram, liver and kidney function. Most of the organs in the pathological tissue section showed no abnormalities, and occasionally vasodilation and congestion and liver tissue necrosis were seen.