1、 Pharmacopoeia standard of acute sub
This product is the dried and mature seed of Impatiens balsamina L., a plant of Impatiens family. The fruits are harvested when they are about to mature in summer and autumn, dried in the sun, and the peel and impurities are removed.
. The seed coat is thin, the cotyledons are gray white, translucent and oily. It is light and bitter.
. The surface of seed coat epidermal cells is irregular, and the vertical wall is wavy and curved. The glandular scale head is spherical, with 4 ～ 5 (～ 12) cells, 22 ～ 60 μ m in diameter, and filled with yellowish brown substances. Calcium oxalate needle crystal bundles exist in mucus cells and are 16-60 μ m long. Endosperm cells are polygonal, with slightly thick walls, containing fatty oil droplets, and often connected with decadent seed coat tissue.
(2) Take 4G of the product powder, add 40ml of acetone, heat and reflux for 1 hour, discard the acetone solution, volatilize the drug residue, add 40ml of water to saturate n-butanol, sonicate for 30 minutes, filter, recover the solvent from the filtrate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test solution. . Then take the reference substance of Impatiens terpenol saponin K and Impatiens terpenol saponin A and add methanol to make a mixed solution containing 1mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 2 μ l of each of the above three solutions, dot them on the same silica gel G thin-layer plate, use chloroform methanol water formic acid (7:3:0.5:0.5) as the developing agent, develop, take out, air dry, spray with 5% vanillin sulfuric acid solution, heat at 105 ℃ until the spots are clear, and inspect under sunlight. .
[inspection] impurities shall not exceed 5% (general rule 2301).
The moisture content shall not exceed 11.0% (the second method of general rule 0832).
The total ash content shall not exceed 6.0% (general rule 2302).
[extract] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 10.0%.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Use acetonitrile as mobile phase A and water as mobile phase B to perform gradient elution according to the following table; Evaporative light scattering detector detection. The number of theoretical plates shall not be less than 3000 according to the peak K of Impatiens terpene tetranol saponin.
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Time (min) mobile phase a (%) mobile phase B (%)

0～15 24→28 76→72

15～25 28 72

25～30 28→40 72→60
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Preparation of reference solution take an appropriate amount of Impatiens terpenol saponin K reference substance and Impatiens terpenol saponin a reference substance, accurately weigh them, add methanol to make solutions containing Impatiens terpenol saponin k0.5mg and Impatiens terpenol saponin a0.25mg per 1ml, respectively.
Preparation of test solution take about 1g of powder (passing through No. 3 screen), weigh accurately, place in Soxhlet extractor, add an appropriate amount of petroleum ether (60 ～ 90 ℃), heat and reflux for 2 hours, discard the petroleum ether liquid, volatilize the solvent from the drug residue, transfer to a corked conical flask, add 50ml of 80% methanol precisely, weigh, heat and reflux for 1 hour, cool, weigh again, use 80% methanol to make up the lost weight, shake well, filter, accurately measure 20ml of the filtrate, recover the solvent to dryness, add an appropriate amount of methanol to dissolve the residue and transfer to a 2ml volumetric flask, add methanol to the scale, shake well, filter, and take the continuous filtrate to get it.
The determination method is to precisely suck 5 μ L and 15 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, determine, and calculate with the external standard two-point method logarithmic equation.
According to the calculation of dried product, the total amount of Impatiens terpene tetraol saponin K (c54h92o25) and Impatiens terpene tetraol saponin A (c48h82o20) shall not be less than 0.20%.
[nature, taste and meridian tropism] slightly bitter, pungent and warm; . Return to lung and liver channels.
[functions and indications] breaking blood, softening and firming, and eliminating accumulation. It is used for symptomatic scrofula, amenorrhea and choking diaphragm.
[usage and dosage] 3-5g.
[note] pregnant women should use it with caution.
[storage] put it in a dry place.

2、 Chemical constituents of acuteness

Contains aesculesterol, parinaric acid, saponin, fatty oil, polysaccharide, protein, amino acid, volatile oil, andquercetin Of polyglycosides andKaempferolFlavonoids such as derivatives of.

3、 Pharmacological action of acuteness

① Effect on uterus
The tincture, Decoction and water extract of Impatiens have obvious excitatory effect on the isolated uterus of nonpregnant rabbits and the isolated uterus of nonpregnant and pregnant guinea pigs at various stages, which is manifested by faster rhythmic contraction, higher tension and even tonic contraction. In the eutopic uterine test of anesthetized rabbits, intravenous or intramuscular injection of 0.05 ～ 0.3 g / kg of water extract can increase the tension and contraction of the uterus. It has inhibitory effect on isolated rabbit intestine, water extract or tincture.

② Contraception
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