1、 Pharmacopoeia standard of Jinguolan
. Excavate in autumn and winter, remove fibrous roots, wash and dry.
[properties] the product is irregular and round, 5-10cm long and 3-6cm in diameter. The surface is brownish yellow or light brown, rough and uneven, with deep wrinkles. It is hard, not easy to break and break. Its cross section is yellowish white, and the duct bundles are slightly radially arranged, with a darker color. The breath is slight and the taste is bitter.
. Stone cells are numerous, light yellow or yellow, rectangular or polygonal, with a diameter of 18-66 µ m, thickened walls on three sides, and calcium oxalate crystals are contained in the cell cavity. The calcium oxalate crystals are square or rectangular, with a diameter of 4-28 µ M. the cork cells are yellowish brown or golden yellow, and appear polygonal and slightly lignified on the surface. There are many starch grains, which are spherical, helmet shaped or polygonal round, with a diameter of 4-40 µ m, and umbilicus herringbone, short arc or dot shaped; Compound granules are composed of 2-5 fractions.
(2) Take 1g of this product powder, add 20ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate, add 2ml of methanol to dissolve the residue, and use it as the test solution. In addition, take gulumbin reference substance and add methanol to make a solution containing 0.5mg per 1ml as the reference substance solution. Test according to thin-layer chromatography (general rule 0502), suck 2-3 µ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use the upper solution of cyclohexane ethyl acetate methanol concentrated ammonia test solution (10:9:6:1) as the developing agent, develop, take out, air dry, spray 10% ethyl sulfate solution, heat at 105 ℃ until the spots are clear, and check under sunlight and ultraviolet light (365nm). In the chromatogram of the test sample, spots or fluorescent spots of the same color appear at the corresponding positions of the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 13.0% (the second method of general rule 0832).
The total ash content shall not exceed 7.0% (general rule 2302).
[extract] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 7.0%.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Acetonitrile water (40:60) was used as mobile phase; The detection wavelength is 210nm. The number of theoretical plates should not be less than 2500 according to the gulumbin peak.
Preparation of reference solution take an appropriate amount of gulumbin reference substance, weigh it accurately, and make a solution containing 0.25mg per 1ml with 70% methanol.
Preparation of test solution take about 0.5g of powder (passing through No. 3 screen), weigh accurately, add 10ml of 70% methanol precisely, weigh, sonicate (power 200W, frequency 59khz) for 20 minutes, cool, weigh again, use 70% methanol to make up the lost weight, shake well, filter, accurately measure 1ml of continuous filtrate, put it into a 10ml measuring bottle, add 70% methanol to the scale, shake well, and get it.
The determination method is to precisely suck 10 µ l of the reference solution and 10 µ l of the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of gulumbin (c2qh22o6) shall not be less than 1.0%.
Decoction pieces
[processing] remove impurities, soak, moisten, cut thick pieces, and dry.
This product is a thick piece of quasi circular or irregular shape. The outer skin is brownish yellow to dark brown, wrinkled and uneven. The cut surface is yellowish white, sometimes grayish brown and sparse radial texture can be seen, and some have cracks. The breath is slight and the taste is bitter.
[identification], [inspection], [extract], [content determination] are the same as those of medicinal materials.
[nature, taste and meridian tropism] bitter, cold. Return to lung and large intestine channels.
[functions and indications] it can clear away heat, detoxify, promote swallowing and relieve pain. For sore throat, carbuncle, gangrene, toxin, diarrhea, dysentery, abdominal pain.
[usage and dosage] 3 ～ 9g. Apply an appropriate amount externally, grind and blow the throat or coat the affected part with vinegar mill.
[storage] store in a dry place to prevent moths.

2、 Chemical constituents of Rhizophora fortunei

The root tuber of Rhizophora fortunei contains palmatine, anticolumbine, isocolumbin, jatrhizine, and tinoside. .

3、 Pharmacological effects of Rhizoma Polygonatum

1. it can atrophy the thymus of young mice and significantly stimulate the secretion of animal pituitary adrenal cortex. The test showed that palmoplantar tetrandrine 25mg/kg subcutaneous injection can cause a significant decrease in the content of vitamin C in the adrenal gland of rats. At the same time, this stimulating effect of ACTH release in rats must have an intact pituitary.

2. in the blood pressure test of cats and dogs, the lower limb perfusion test of toads, and the ex vivo seminal vesicle test of rats, palmoplantar tetrandrine has the effect of anti epinephrine.

3. it has obvious anti serotonin effect in uterine muscle, rat colon and isolated rats.

4. using rabbit whole blood and bromothymol blue as the indicator, it is proved that tetrandrine has a strong anti cholinesterase effect.

5. palmoplantar tetrandrine has an excitatory effect on the uterus of nonpregnant rabbits.

6. it has strong effect on Staphylococcus aureus and anti acid Mycobacterium.

7. hypoglycemic effect: oral administration of its aqueous or alcoholic extract to rabbits and rats can reduce fasting blood glucose and increase glucose tolerance (if it is taken orally for 1 month, the tolerance test will deteriorate). The bitter ingredient in the water extract seems to be an effective ingredient. Although the hypoglycemic effect is not obvious, it can inhibit hyperglycemia caused by epinephrine; Organic solvent (ether, chloroform or petroleum ether) extracts are ineffective. . 8. anti tumor: qingniudan Decoction 4g/kg has inhibitory effect on sarcoma 180 in mice.