1、
This product is the dried and mature seeds of Prunus humilis bge., Prunus japonica thunb., or Prunus pedunculata maxim., a Rosaceae plant. . Harvest the mature fruits in summer and autumn, remove the pulp and core shell, take out the seeds, and dry them.
[character] the plums are oval, 5 ～ 8mm long and 3 ～ 5mm in diameter. The surface is yellowish white or light brown, with one end pointed and the other end blunt and round. There is a linear hilum on one side of the tip, a dark chalaza in the center of the round end, and multiple longitudinal vascular bundle veins upward at the self chalaza. The seed coat is thin, cotyledons 2, milky white, oily. The breath is slight, and the taste is slightly bitter.
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[identification] take 0.5g of this product powder, add 10ml of methanol, sonicate for 15 minutes, filter, evaporate the filtrate, add 2ml of methanol to dissolve the residue, and use it as the test solution. In addition, take amygdalin reference substance and add methanol to make a solution containing 4mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 2 μ l each of the above two solutions, dot them on the same silica gel G thin-layer plate respectively, use the lower layer solution of chloroform ethyl acetate methanol water (3:8:5:2) placed below 10 ℃ as the developing agent, develop, take out, dry, spray phosphomolybdic acid sulfuric acid solution (2G phosphomolybdic acid, add 20ml water to dissolve, then slowly add 30ml sulfuric acid, mix well), and heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 6.0% (the second method of general rule 0832).
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The acid value shall not exceed 10.0.
Carbonyl value shall not exceed 3.0.
The peroxide value shall not exceed 0.050.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler, and acetonitrile water (12:88) was used as mobile phase; The detection wavelength was 210nm. The number of theoretical plates should not be less than 3000 based on the peak of amygdalin.
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Prepare the test sample solution, take about 0.2g of the powder (passing through No. 2 screen), accurately weigh it, place it in a corked conical flask, precisely add 20ml of methanol, weigh it, heat and reflux for 1 hour, cool it, then weigh it, use methanol to make up the weight lost, shake it well, filter it, accurately measure 1ml of the continued filtrate, place it in a 10ml measuring flask, add methanol to the scale, shake it well, filter it, and take the continued filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
The content of amygdalin (c20h27no11) in this product shall not be less than 2.0% according to the dry product.
Decoction pieces
[processing] remove impurities. Mash when needed.
[character], [identification], [inspection], [content determination] are the same as those of medicinal materials.
[nature, taste and meridian tropism] pungent, bitter, sweet and smooth. Return to spleen, large intestine and small intestine.
[functions and indications] moisten the intestines and relieve constipation, lower Qi and promote diuresis. It is used for dry intestines, food accumulation and qi stagnation, abdominal distension, constipation, edema, beriberi, and urination.
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[note] pregnant women should use it with caution.
[storage] store in a cool and dry place to prevent moths.

2、

This product mainly containsAmygdalin, salidroside a, salidroside BKaempferitrin , rosarin a, IR-A, ir-bUrsolic acid。

3、 Pharmacological effects of Prunus mume

Thin layer chromatography and soluble protein electrophoresis analysis are of identification significance; Salidroside has a strong purgative effect; Saponins have antitussive, expectorant and antiasthmatic effects; The extracted protein components ir-ai and ir-b have anti-inflammatory and analgesic effects by intravenous injection.