1、 Pharmacopoeia standard of Bergenia chinensis
  This product is the dried rhizome of Bergenia purpurascens (hook. F. et thoms.) engl., a plant of Saxifragaceae. Excavate in autumn and winter, remove leaf sheaths and impurities, and dry in the sun.
. It is solid and brittle and easy to break. The cross-section is white or pink, slightly silty, and some cross-sections have reticular fissures, with dotted vascular bundles arranged in a ring near the edge. It tastes bitter and astringent.
[identification] (1) cross section of the rhizome of this product: there are often residual epidermal cells, and the cork layer is composed of more than 10 rows of flat cells. The cambium is obvious. The vascular bundle is tough on the outside, and the pericycle fibers are occasionally seen on the outside. . The medulla is broad. Parenchyma cells contained calcium oxalate clusters, starch granules and brown matter.
The powder is brownish yellow. Calcium oxalate has many cluster crystals with a diameter of 15-58 µ M. the starch grains are oval or pear shaped, and the two ends are usually slightly pointed, with a diameter of 3-10 µ m and a length of 8-20 µ M. the laminar lines and umbilical points are not obvious. The ducts are mostly reticulated, with a diameter of 13-35 µ M. the epidermal cells are reddish brown and appear polygonal or quasi rectangular on the surface. The surface of cork cells was polygonal.
(2) Take 0.2g of this product powder, add 20ml of methanol, sonicate for 40 minutes, cool, filter, and take the filtrate as the test solution. In addition, take bergenin reference substance and arbutin reference substance, add methanol to make a mixed solution containing 0.2mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 5 µ l of each of the above two solutions, dot them on the same silica gel gf254 thin-layer plate respectively, develop them twice with chloroform ethyl acetate methanol (4:4:1.5) as the developing agent, take them out, dry them, and view them under UV light (254nm). ; Spray the mixed solution of 2% ferric chloride solution and 1% potassium ferricyanide solution (1:1) again. In the chromatogram of the test sample, spots of the same color will appear on the corresponding positions of the chromatogram of Bergenin reference substance and arbutin reference substance.
[inspection] the moisture content shall not exceed 12.5% (the second method of general rule 0832).
The total ash content shall not exceed 7.5% (general rule 2302).
[extract] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 36.0%.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Methanol water (20:80) was used as mobile phase; The detection wavelength is 275nm. The number of theoretical plates should not be less than 4500 according to the bergenin peak.
Preparation of reference solution take an appropriate amount of Bergenin reference, weigh accurately, add 80% methanol to make a solution containing 0.35mg per 1ml.
Preparation of the test solution take 0.2g of the powder (passing the second sieve), accurately weigh it, put it into a 50ml volumetric flask, add an appropriate amount of 80% methanol, sonicate (power 100W, frequency 25kHz) for 40 minutes, cool it, add 80% methanol to dilute to the scale, shake it well, filter it, and take the secondary filtrate.
The determination method is to precisely suck 10 µ l of the reference solution and 10 µ l of the test solution, inject them into the liquid chromatograph, and determine.
The content of Bergenin (c14h16o9) in this product shall not be less than 8.2% according to the dry product.
[processing] remove leaf sheaths and impurities and dry them in the sun.
[nature, taste and meridian tropism] bitter, astringent and flat. .
[functions and indications] it can astringe and stop diarrhea, stop bleeding and cough, and relax muscles and activate collaterals. It is used for diarrhea, dysentery, loss of appetite, bleeding from internal and external injuries, tuberculosis cough, tracheitis cough, rheumatic pain, traumatic injury.
[usage and dosage] 6-12g. Suitable for external use.
[storage] store in a cool and dry place.

2、 Chemical constituents of Bergenia chinensis

Whole grass containsBergenin(bergenin)。 In addition, it contains 6-o-galloylated arbutin, 4,6-di-o-galloylated arbutin, 2,4,6-tri-o-galloylated arbutin, 2,3,4,6, - tetra-o-galloylated arbutin.

3、

The root of Pakchoi in thick leaf rock contains arbutin, so it has antibacterial and anti-inflammatory effects; It contains tannin, so it has astringent effect. It can be used for non infectious inflammation of digestive tract, and also for cervical erosion. It has also been reported that the total tannin content is 19.4%, and its extract can inhibit Shigella at a concentration of 1 ∶ 320-480 in the test tube. It also contains vitamin P-like substances. Long term oral administration of water extract to rats can increase capillary resistance. Long term oral administration of large doses does not cause toxic reactions, neither affecting the general state of rats, nor causing pathological changes in the digestive tract or parenchymal organs.