1、 Standard of traditional Chinese herbal medicine
This product is the dried whole grass of Sedum sarmentosum Bunge, a Crassulaceae plant. Harvest in summer and autumn, remove impurities and dry.
[character] the stem of this product is thin, with a length of more than 20cm. Thin adventitious roots can be seen on some nodes. . The breath is slight, and the taste is slightly bitter.
[identification] (1) the transverse section of the stem of this product: the epidermal cells are rectangular, the outer wall is thickened, and the inner layer has about 10 rows of parenchymal cells. The middle column is small, the outer vascular bundle is tough, and the duct is round. The pulp is triangular, with polygonal cells, thick walls, and non lignified. There are reddish brown secretions in the adjacent phloem cells and myeloid cells.
(2) Take 3G of this product powder, add 20ml of methanol, sonicate for 30 minutes, filter, and take the filtrate as the test solution. Another 3G of Sedum sarmentosum reference medicinal material was prepared into the reference medicinal material solution by the same method. According to the test of thin-layer chromatography (general rule 0502), suck 3 µ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use cyclohexane ethyl acetate (40:1) as the developing agent, develop, take out, air dry, spray 5% phosphomolybdic acid ethanol solution, and heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots of the same color appear on the corresponding position of the chromatogram of the control medicinal material.
[inspection] the moisture content shall not exceed 13.0% (the second method of general rule 0832).
Acid insoluble ash content shall not exceed 6.0% (general rule 2302).
[extract] determined by the hot leaching method under the determination method of water-soluble extract (general rule 2201), it shall not be less than 20.0%.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; ; The detection wavelength is 360nm. The number of theoretical plates should not be less than 3000 according to the quercetin peak.
Preparation of reference solution take an appropriate amount of quercetin reference substance, kaempferol reference substance and isorhamnetin reference substance, weigh accurately, and add methanol to make a mixed solution containing 15 µ g quercetin, 5 µ g kaempferol and 5 µ g isorhamnetin per 1ml.
Preparation of test solution take about 0.5g of powder (passing through No. 2 screen), weigh accurately, add 25ml of methanol-25% hydrochloric acid solution (4:1) mixed solution precisely, weigh, heat and reflux for 1 hour, cool, weigh again, make up the lost weight with methanol-25% hydrochloric acid solution (4:1) mixed solution, shake well, filter, and take the filtrate.
Determination method precisely suck 10 µ l of the reference solution and 10 µ l of the test solution into the liquid chromatograph for determination.
The total amount of quercetin (C15H10O7), kaempferol (c15h10o6) and isorhamnetin (c16h12o7) in this product shall not be less than 0.10% according to the calculation of dry product.
Decoction pieces
[processing] remove impurities and cut into sections.
. Thin adventitious roots can be seen on some nodes. 3. The impeller is raw, and the blade is oblanceolate to rectangular circular, green. The breath is slight, and the taste is slightly bitter.
[identification] (except for the transverse section of the stem), [inspection], [extract], [content determination] are the same as those of medicinal materials.
[nature, taste and meridian tropism] sweet, light and cool. It belongs to the channels of liver, gallbladder and small intestine.
[functions and indications] it can remove dampness and jaundice, clear away heat and detoxify. It is used for damp heat jaundice, unfavorable urination, carbuncle and sores.
[usage and dosage] 15 ～ 30g.
[storage] put it in a dry place.

2、 Chemical constituents of Sedum sarmentosum

（methylisopelletierine）, dihydroisopelletierine, 3-formyl-1,4-dihydroxy-dihydropyran, n-methyl-2 β - hydroxypropyl piperidine, sarmentosine,β - sitosterol（β-sitosterol），D-MannitolD-Mannitol, amino acids, glucose, fructose and sedoheptulose.

3、 Pharmacological effects of Sedum sarmentosum

（1） Liver protection
      Sedum sarmentosum has obvious protective effect on subacute liver injury induced by CCl4 in rats, and it can significantly reduce the retention rate of serum transaminase and sodium sulfabromophthalein. The γ - globulin of the treatment group was significantly lower than that of the control group, and the degree of hepatic pulmonary fibrosis was lighter than that of the control group.
      Hepatitis B model (DHBV positive duck) was gavaged with Sedum sarmentosum extract once a day. After 6 weeks, the effect of enzyme reduction was significant and rapid, but there was rebound phenomenon after drug withdrawal, and the effect was very unstable. Pathological observation showed that after 6 weeks of continuous administration, the liver was significantly improved, the inflammatory exudation was significantly resolved, the inflammatory cell infiltration in the manifold disappeared, and the necrotic foci were significantly reduced, but there was no effect on DHBV. The results showed that the enzyme reducing effect of Sedum sarmentosum was mainly through improving liver cell injury, and its effective component was Sedum sarmentosum glycoside.

（2） Immunosuppression
      At a high dose (500mg / kg), sargassoside has a significant inhibitory effect on cellular immunity in rats and mice: it reduces thymocytes in C57 pure line mice It also inhibited the number of plaque forming cells in the spleen of mice.

（3） Other
      Sedum sarmentosum has broad-spectrum antibacterial effect.