1、 Pharmacopoeia standard of Radix Polygoni Multiflori
Why is this product processed from Radix Polygoni Multiflori.
[preparation method] take slices or blocks of Polygonum multiflorum, mix them with black soybean juice according to the stewing method (general rule 0213), place them in a suitable non-ferrous container, and stew until the juice is absorbed; Or according to the steaming method (general rule 0213), steamed or mixed with black soybean juice, steamed until brown inside and outside, or sun dried until semi dry, sliced, and dried.
For each 100kg of Polygonum multiflorum tablets (pieces), use 10kg of black beans.
Black bean juice preparation method: take 10kg of black beans, add appropriate amount of water, boil for about 4 hours, boil for about 15kg, add water to boil for about 3 hours, boil for about 10kg, and combine to obtain about 25kg of black bean juice.
. It is hard, with horny cross-section, brown or black. It is slightly sweet and bitter.
[identification] according to [identification] (2) test under Polygonum multiflorum, the same results were obtained.
[inspection] the moisture content shall not exceed 12.0% (the second method of general rule 0832).
The total ash content shall not exceed 9.0% (general rule 2302).
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[content determination] stilbene glycoside avoid light.
Take about 0.2g of this product powder (passing through No. 4 screen), weigh it accurately, and determine it according to the method of Polygonum multiflorum Thunb.
According to the calculation of dry product, the content of 2,3,5,4 '- tetrahydroxystilbene-2-o - β - D-glucoside (c20h22o9) shall not be less than 0.70%.
Free onion quinone was determined by HPLC (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol-0.1% phosphoric acid solution (80:20); The detection wavelength is 254nm. The number of theoretical plates should not be less than 3000 according to the emodin peak.
Preparation of reference solution take an appropriate amount of emodin reference and emodin methyl ether reference, weigh accurately, add methanol to make a solution containing 80 μ g of emodin and 40 μ g of emodin methyl ether per 1ml, respectively.
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The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
This product is calculated as a dry product, and the free anthraquinone contained shall not be less than 0.10% based on the total amount of emodin (c15h10o5) and emodin methyl ether (c16h12o5).
[nature, taste and meridian tropism] bitter, sweet, astringent, mild. Return to liver, heart and kidney channels.
. It is used for blood deficiency, yellowing, dizziness, tinnitus, early white beard, weak waist and knees, numbness of limbs, metrorrhagia and metrorrhagia, and hyperlipidemia.
[usage and dosage] 6 ～ 12g.
[storage] store in a dry place to prevent moths.

2、 Chemical constituents of Radix Polygoni Multiflori

The root tuber contains anthraquinones, mainlyEmodin（emodin),Chrysophanol(chrysophanol) as well asEmodin methyl ether（physcion），Rhein（rhein）， . It also contains stilbene compounds:Resveratrol（resveratrol）， Piceid, 2,3,5,4 '- tetrahydroxystilbene-2-o - β - d-glu-copyranoside, 2,3,5,4' - tetrahydroxystilbene-2-o-glucoside-2 ″ - o-gallate ER), 2,3,5,4 '- tetrahydroxystilbene-2-o-glucoside-3 ″ - o-gallate (2,3,5, 4’－tetrahydroxystilbene－2－O-β-D－glucopyrano-side-3″-monogalloyl ester)。 Also containsgallic acid（gallic acid），Catechin（catechin），Epicatechin(epicatechin), 3-o-galloyl (-) - catechin [3-o-galloyl (-) - catechin], 3-o-galloyl (-) - epicatechin [3-o-galloyl (-) - epicatechin], 3-o-galloyl-procyanidin B-2, 3,3 '- di-o-galloyl-procyanidin B-2, andβ - sitosterol（β-sitosterol），

3、

1. effect on central nervous system: in the test of jumping platform and avoiding the dark in mice, the 60g/kg Decoction of Polygonum multiflorum by gavage has a significant antagonistic effect on the impairment of memory acquisition in mice caused by scopolamine.

2. reducing blood lipids and anti atherosclerosis: 0.084, 0.84, 8.4g/kg alcohol extract of Polygonum multiflorum by gavage for 6 weeks can inhibit the rise of total cholesterol, triglycerides, free cholesterol and cholesterol esters in plasma of quails with hyperdactyly, and delay the formation and development of cerebral atherosclerosis.

3. effect on hematopoietic system: 1g/ml and 0.2g/ml of Polygonum multiflorum Decoction and alcohol precipitate were subcutaneously injected. It was proved by in vivo diffusion box method that it can promote the growth of murine bone marrow granulocyte progenitor cells. Raw Radix Polygoni multiflori and Prepared Radix Polygoni Multiflori (prepared by high-pressure method) were gavaged with 30g/kg warm extract respectively for 14 days. For hemorrhagic blood deficiency mice, Prepared Radix Polygoni Multiflori could increase the number of red blood cells and hemoglobin content in blood, but raw Radix Polygoni Multiflori had no obvious effect.

4. effect on immune function: raw Polygonum multiflorum, black bean juice mixed with steamed 32 hours Polygonum multiflorum (product 1), black bean juice nine steamed nine sun dried Polygonum multiflorum (product 2) Decoction, 6g/kg by gavage, for 7 consecutive days, the experimental results showed that product Ⅰ could enhance the ability of mouse peritoneal macrophagy of chicken red blood cells, and resist the inhibitory effect of prednisolone on macrophagy. Product Ⅰ increased red blood cell immunity, increased the rate of C3b receptor rosette in mouse red blood cells, decreased the rate of immune complex rosette, and antagonized the immunosuppressive effect of prednisolone. The effect of raw Polygonum multiflorum Thunb and product II was not significant.

. Polygonum multiflorum injection 0.16, 0.03, 0.01mg/ml in vitro culture method can significantly enhance the DNA replication of neonatal rat hepatocytes.

6. anti aging: 0.1 and 1.0g / ml of water-soluble components of Polygonum multiflorum can reduce the activity of B-type monoamine oxidase (MAO-B) in the brain of rats and mice (MAO-B activity in the brain of older people and older animals is increased). Polygonum multiflorum Decoction (1g/ml, 0.5ml/ mouse) by gavage for 7 consecutive days can increase the weight of adrenal glands and the activity of superoxide dismutase (SOD) in aged mice.

7. anti blood aggregation: Polygonum multiflorum powder 4g/kg fed to rats in the feed for 8 weeks can resist ADP induced platelet aggregation. 8. toxicity: the LD50 of alcohol cold extract of Radix Polygoni Multiflori to mice is 5.5g/kg by intraperitoneal injection. No mice died after intraperitoneal injection of 300g/kg alcohol cold extract of Radix Polygoni Multiflori. The LD50 of raw Polygonum multiflorum alcohol percolation solution to mice was 50g/kg, and the prepared Polygonum multiflorum alcohol percolation solution was 1000g/kg, and no mice died.