1、 Pharmacopoeia standard of tiannanxing
 
. This product is processed from tiannanxing.
. Put ginger slices and alum in the pot and add appropriate amount of water to boil, pour them into Arisaema and boil them until there is no dry heart. Take them out, remove ginger slices, air them to 40-60% dry, cut thin slices and dry them. For every 100kg of Arisaema, 12.5kg of ginger and 12.5kg of alum are used respectively.
[properties] this product is a thin, quasi circular or irregular shape. Yellow or light brown, brittle and fragile, with horny section. It has slight air, astringent taste and slight numbness.
[identification] (1) the powder of this product is grayish yellow or yellowish brown. . Calcium oxalate needle crystals are scattered or in bundles, with a length of 6-35 μ M. Threaded conduit and ring conduit.
(2) Take 5g of this product powder, add 50ml of ethanol, heat and reflux for 1.5 hours, filter, evaporate the filtrate, add 10ml of diethyl ether to the residue for ultrasonic treatment for 5 minutes, filter, repeat the treatment of the residue with diethyl ether twice, combine the ether solution, evaporate to dryness, add 0.5ml of methanol to the residue to dissolve it, and use it as the test solution. Another 0.5g of dried ginger reference material was prepared into the reference material solution by the same method. . In the chromatogram of the test sample, the main spot with the same color appears at the position corresponding to the chromatogram of the control medicinal material.
[inspection] the moisture content shall not exceed 12.0% (the second method of general rule 0832).
The total ash content shall not exceed 4.0% (general rule 2302).
Take about 2G of alum powder (passing through No. 4 screen), weigh accurately, put it in a crucible, heat it slowly, when it is completely carbonized, gradually increase the temperature to 450 ℃, ash it for 4 hours, cool it, carefully add 10ml of dilute hydrochloric acid into the crucible, cover the crucible with a surface dish, heat it on a water bath for 20 minutes, wash the surface dish with 5ml of hot water, incorporate the washing solution into the crucible, filter it, wash the filter residue and crucible with 25ml of water in several times (combine the filtrate and washing solution, add a drop of methyl red indicator solution, shake it well, and then drop ammonia test solution until the solution turns from red to yellow, add acetic acid ammonium acetate for slow flushing add 25ml of ethylenediamine tetraacetic acid disodium titration solution (0.05mol/l) precisely, boil for 3-5 minutes, cool it, add 1ml of xylenol orange indicator solution, and use zinc titration solution (0.05m0l/l) Titrate until the solution changes from yellow to orange, and correct the titration result with blank test. Every 1ml of disodium ethylenediamine tetraacetate titrant (0.05mol/l) is equivalent to 23.72mg of potassium aluminum sulfate [kal (SO4) 2 · 12h2o] in water.
This product is calculated as dry product, and the alum content is calculated as potassium aluminum sulfate with water [kal (SO4) 2 · 12h2o], which shall not exceed 12.0%.
[content determination] take about 0.5g of this product powder (passing through No. 4 screen), accurately weigh it, place it in a corked conical flask, precisely add 25ml of 60% ethanol, close the stopper, weigh it, sonicate (power 250W, frequency 40KHz) for 45 minutes, cool it, weigh it again, use 60% ethanol to make up for the lost weight, shake it well, filter it, accurately measure 1-5ml of the continuous filtrate, place it in a 10ml measuring bottle, according to the method under the preparation of the standard curve under the item of tiannanxing [content determination], measure the absorbance according to law from "adding triethylamine solution", and read out the celery in the test solution from the standard curve.Calculate the weight of vegetable.
This product is calculated as a dry product, and the total flavone content is calculated as apigenin (c15h10o5), which should not be less than 0.050%.
[nature, taste and meridian tropism] bitter, pungent and warm; . .
[functions and indications] dry dampness and dissipate phlegm, dispel wind, stop spasm, disperse nodules and reduce swelling. It is used for persistent phlegm cough, wind phlegm dizziness, stroke phlegm congestion, mouth and eye deviation, hemiplegia, leprosy, convulsion, tetanus; For external use, it can treat carbuncle and swelling, snake and insect bites.
[usage and dosage] 3 ~ 9g.
[note] pregnant women should use it with caution.
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2、 Chemical constituents of Rhizoma Arisaema

D-Mannitol（D-Mannitol）、Trigonelline（Trigonelline）、Colchicine（Colchicine）、 CholineStachydrine（Stachydrine）、 Triacontanoic acidβ - sitosterol（Beta-Sitosterol）、Stigmasterol（Stigmasterol）、Campesterol（Campesterol）、Ethyl gallate（Gallic Acid Ethyl Ester）、 Tetradecane,Carotene（Eleutheroside A）、gallic acid（Gallic acid）、 Hexacosanoic acid, which also containspalmitic acid(palmitic acid)，l-serine (L-serine)，L-valine (L-valine)，L-lysine(L-lysine)，L-Proline More than 30 kinds of amino acids such as L-proline and more than 20 kinds of inorganic trace elements such as magnesium, aluminum, zinc, copper, selenium, vanadium and cobalt.

3、

1. anticonvulsant effect: intraperitoneal injection of tiannanxing Decoction (3g/kg) can significantly resist the convulsion caused by strychnine, pentamethene tetrazolium and caffeine, but can not resist the onset of electric shock, and its anticonvulsant intensity varies according to different varieties. However, there are also reports that tiannanxing can not resist the convulsion and death caused by strychnine, but can resist the convulsive death caused by nicotine, and can still eliminate its muscle tremor symptoms; For convulsions caused by tetanus toxin intramuscular injection in mice, tiannanxing can delay the effect of animal death.

2. sedative and analgesic effects: after intraperitoneal injection of tiannanxing decoction, rabbits and rats showed decreased activity, quiet, and blunted righting reflex. It can also prolong the sleep time of adult barbiturate in mice, and has obvious analgesic effect.

3. expectorant effect: the phenol red excretion method in mice was used for the experiment, which showed that tomorrow Nanxing water agent had expectorant effect, and the amount of phenol red excreted from the respiratory tract in the administration group was 150% and 170% of that in the control group, respectively.

4. anti tumor effect: the alcohol treated preparation of the water extract of fresh Arisaema chinensis (unidentified variety) has an inhibitory effect on HeLa cells in vitro, and has a certain inhibitory effect on mouse experimental tumors such as sarcoma S180, HCA solid type and U14, and it is confirmed that D-Mannitol may be an effective anti-cancer ingredient.

5. antioxidant effect: the two alkaloids contained in Arisaema have different activities of scavenging superoxide anion free radicals and inhibiting lipid peroxidation of liver mitochondria.

. The water extraction and alcohol precipitation preparation of fresh Arisaema has inhibitory effect on HeLa cells in vitro. It is effective against experimental tumors in mice, and has certain inhibitory effects on S180, HCA solid type, U14, etc.