1、 Pharmacopoeia standard of Albizzia julibrissin
Albizzia julibrissin
Hehuanhua
ALBIZIAE FLOS
. In summer, flowers are harvested on sunny days or when flower buds are formed, and dried in time. The former is known as "Albizzia flower" and the latter is known as "Albizzia rice".Hehuanhua
ALBIZIAE FLOS
[character] Albizzia julibrissin Capitulum, shrunk into a mass. The total pedicel is 3 ~ 4cm long, sometimes separated from the inflorescence, yellowish green, with longitudinal lines, and is sparsely hairy. The flowers are densely hairy, slender and curved, 0.7 ~ 1cm long, yellowish or yellowish brown, without or almost without pedicels. Calyx tubular, apex with 5 small teeth; The corolla tube is about twice as long as the calyx tube, the apex is 5-lobed, and the lobes are lanceolate; The stamens are numerous, the filaments are slender, yellowish brown to yellowish brown, the lower part is united, the upper part is separated, and extends out of the corolla tube. .
Albizzia rice It is mallet shaped, 2-6mm long, most of which are about 2mm in diameter, pale yellow to yellowish brown, all of which are hairy, and the pedicel is very short or absent. Calyx tubular, apex with 5 small teeth; Corolla not open; . .
[identification] (1) the powder of this product is grayish yellow. Non glandular hairs are single-cell, 81-447 µ m in length. Calcium oxalate crystals are numerous and exist in parenchyma cells, with a diameter of 3-31 µ M. the composite pollen grains are oblate, 16 syncytia, with a diameter of 81-146 µ m, and 8 peripheral; A single split is square like or long spherical.
(2) Take 0.6g of this product powder, add 30ml of 70% ethanol, heat and reflux for 1 hour, filter, evaporate the filtrate, add 25ml of water to dissolve the residue, shake and extract with water saturated n-butanol twice, 30ml each time, combine n-butanol solution, evaporate to dryness, add 10ml of methanol to dissolve the residue, and use it as the test solution. . Then take quercitrin reference substance and add methanol to make a solution containing 0.5mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 1 µ l of each of the above three solutions, dot them on the same polyamide film, use the upper solution of toluene ethyl acetate 88% formic acid water (1:8:1:1) as the developing agent, develop, take out, dry, spray aluminum trichloride test solution, blow with hot air for about 1 minute, and view under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample.
; Not more than 2% (general rule 2301).
Moisture Not more than 15.0% (the second method of general rule 0832).
Total ash Not more than 10.0% (general rule 2302).
Acid insoluble ash Not more than 3.0% (general rule 2302).
[extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), using dilute ethanol as solvent, it shall not be less than 25.0%.
[content determination] determine according to HPLC (general rule 0512).
Chromatographic conditions and system suitability test Octadecylsilane bonded silica gel was used as filler; ; The detection wavelength was 256nm. The number of theoretical plates should not be less than 3000 according to quercitrin peak.
Preparation of reference solution Take an appropriate amount of quercitrin reference substance, accurately weigh it, add dilute ethanol to make a solution containing 60 µ g per 1ml.
Preparation of test solution Take about 0.25g of this product powder (passing through No. 3 screen), weigh it accurately, place it in a corked conical flask, add 50ml of dilute ethanol precisely, weigh it, heat and reflux for 30 minutes, cool it, weigh it again, make up the lost weight with dilute ethanol, shake it well, filter it, and take the filtrate.
Assay Precisely suck 10 µ l of the control solution and 10 µ l of the test solution respectively, inject them into the liquid chromatograph, and determine.
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[nature, taste and meridian tropism] sweet and smooth. Heart returning and liver meridian.
[functions and indications] relieve depression and calm nerves. Used for restlessness, depression and insomnia.
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2、 Chemical constituents of Albizzia julibrissin
Twenty five aromatic components were identified in the flowers, and the main aromatic component was linalooloxide,Linalool(linalool), . In addition, it also containsCyanidin-3-glucoside chloride(Cyanidin-3-glucoside chloride)。
3、 Pharmacological effects of Albizzia julibrissin
. Albizzia julibrissin decoction has no obvious changes in EEG and no anti convulsive effect in mice induced by pentylenetetrazol.