1、 Pharmacopoeia standard of Phellodendron amurense
Phellodendron amurense
Guanhuangbo
PHELLODENDRI AMURENSIS CORTEX
[properties] the product is in a plate-shaped or shallow groove shape, with different length and width, and 2-4mm thick. The external surface is yellowish green or light brownish yellow, relatively flat, with irregular longitudinal cracks, small and rare skin hole marks, and occasionally gray white coarse skin remains; The inner surface is yellow or yellowish brown. It is light, hard, fibrous in cross section, and some are split and layered, bright yellow or yellowish green. The breath is weak, the taste is extremely bitter, and the chewing is sticky.
[identification] (1) the powder of this product is green yellow or yellow. The fiber is bright yellow, 16-38 µ m in diameter, often in bundles, and the surrounding cells contain calcium oxalate crystals, forming crystal fibers; The wall of crystalline cells is lignified and thickened. The stone cells are bright yellow, quasi round or spindle shaped, 35-80 µ m in diameter, and some are branched, with thick walls and obvious laminations. The diameter of calcium oxalate square crystal is about 24 µ M.
(2) . Another 0.2g of Phellodendron amurense reference medicinal material was taken, and the reference medicinal material solution was prepared by the same method. Then take the reference substance of Phellodendron and add ethyl acetate to make a solution containing 0.6mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 5 µ l of each of the above three solutions, dot them on the same silica gel G thin-layer plate, use petroleum ether (60 ～ 90 ℃) - ethyl acetate (1:1) as the developing agent, develop, take out, dry, spray 10% sulfuric acid ethanol solution, and heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding positions of the chromatogram of the control medicinal material and the chromatogram of the control sample.
[check] moisture Not more than 11.0% (the second method of general rule 0832)
Total ash Not more than 9.0% (general rule 2302).
[extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), 60% ethanol shall be used as solvent, which shall not be less than 17.0%.
[content determination] determine according to HPLC (general rule 0512).
; Octadecylsilane bonded silica gel was used as filler; Acetonitrile was used as mobile phase a, and 0.1% phosphoric acid solution (sodium dihydrogen phosphate was added to reach the concentration of 0.02moi/l) was used as mobile phase B. gradient elution was performed as specified in the following table; The detection wavelength was 345nm. The number of theoretical plates should not be less than 4000 according to the peak of berberine hydrochloride.
Preparation of reference solution Take an appropriate amount of berberine hydrochloride reference substance and bamatine hydrochloride reference substance, weigh accurately, add 60% ethanol to make a mixed solution containing 50 µ g per 1ml.
Preparation of test solution Take about 0.2g of this product powder (passing through No. 3 screen), weigh it accurately, put it into a 50ml volumetric flask, add 40ml of 60% ethanol, sonicate (power 250W, frequency 40KHz) for 45 minutes, cool it, add 60% ethanol to the scale, shake it well, filter it, and take the continuous filtrate.
Assay Precisely suck 10 µ l of the control solution and 10 µ l of the test solution respectively, inject them into the liquid chromatograph, and determine.
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Decoction pieces
[processing] Phellodendron amurense .
This product is filiform. The surface is yellowish green or light brownish yellow, relatively flat. The inner surface is yellow or yellowish brown. The cut surface is bright yellow or yellowish green, and some are layered in sheets. The breath is slight and the taste is extremely bitter.
[identification], [inspection], [extract], [content determination] are the same as those of medicinal materials.
; Take Phellodendron amurense silk and fry it according to the salt water roasting method (general rule 0213). This product is shaped like Phellodendron amurense silk, dark yellow, and occasionally has focal spots. Slightly salty.
[check] moisture .
Total ash No more than 14.0% for the same medicinal materials.
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Phellodendron amurense charcoal Take Phellodendron amurense silk and stir fry it according to the method of charcoal frying (general rule 0213) until the surface is burnt black.
. Light and brittle. The taste is slightly bitter and astringent.
[identification] (except for the superfine powder) is the same as that of medicinal materials.
[nature, taste and meridian tropism] bitter, cold. Return to kidney and bladder meridian.
[functions and indications] clear away heat and dampness, remove fire and steam, detoxify and treat sores. It is used for damp heat diarrhea, jaundice and red urine, vaginal itching, hot shower and pain, flaccid feet, bone steaming and fever, night sweats, spermatorrhea, sore swelling and toxin, eczema and wet sores. Phellodendron amurense in salt pass can nourish yin and reduce fire. .
[usage and dosage] 3 ～ 12g. Suitable for external use.
[storage] store in a ventilated and dry place to prevent moisture.
2、 Chemical constituents of Phellodendron amurense
Bark containsberberine、Jatrorrhizine、Magnoliane、Berberine、 N-methyl macromaltine, palmatine, Dauricine and other alkaloids, andBerberine, phellodendron, lactide, Phellodendron acid, green fluorescent acid, 7-dehydroestigmasterol, &Beta sitosterol、Campesterol。
3、 Pharmacological effects of Phellodendron amurense
Clearing heat and drying dampness, purging fire and removing steam, detoxifying and treating sores. It is a heat clearing and dampness drying medicine under the classification of heat clearing medicine.