Siphonostegia chinensis
1、 Pharmacopoeia standard of Beiliu jinu       This product is the dried whole grass of siphonostegia chinensis benth., a Scrophulariaceae plant. Harvest in autumn, remove impurities and dry in the sun.
      [character] the product is 30 ～ 80cm long and all are short haired. The root is short and curved, slightly branched. The stem is cylindrical, ribbed, and some have branches on the upper part, with brown or black brown surface; It is brittle and easy to break, with yellowish white section, hollow or white pulp. . The raceme is terminal, the flower has short stems, the calyx is long tubular, yellowish brown to black brown, with obvious 10 longitudinal edges, the apex is 5-lobed, and the Corolla is brownish yellow, with many falling off. Capsule narrowly ovate elliptic, slightly shorter than calyx, brown black. The seeds are fine. The air is weak and the taste is light.
      [identification] (1) cross section of the stem of this product: non glandular hairs with 2-4 cells can be seen on the epidermis. The cortex is composed of 2-4 rows of cells. The fibers of the columella are circular. Phloem is narrow. The cambium is not obvious. . The parenchyma cells of the pulp are closely arranged, and some cells have fine pits.
      (2) Take 2G of this product powder, add 20ml of methanol, sonicate for 30 minutes, filter, and concentrate the filtrate to 1ml as the test solution. In addition, take luteolin reference substance and add methanol to make a solution containing 1mg per 1ml as the reference substance solution. . In the chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions of the chromatogram of the control sample.
      [check] moisture   .
      Total ash   Not more than 8.0% (general rule 2302).
      [extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), 70% ethanol shall be used as solvent, which shall not be less than 10.0%.
      [content determination] determine according to HPLC (general rule 0512).
      ; Octadecylsilane bonded silica gel was used as filler; ; The detection wavelength of verbascoside was 310nm, and the detection wavelength of luteolin was 350mn. The number of theoretical plates should not be less than 3000 according to the peak of verbascoside.
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      Time (min) mobile phase a (%)         Mobile phase B (%)
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    0～15                 33               6715～30         33→60            67→40
    30～40                60               40
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      Preparation of reference solution   Take an appropriate amount of luteolin reference substance and verbascoside reference substance, accurately weigh them, add methanol to make a mixed solution containing 70 μ g luteolin and 0.25mg verbascoside per 1ml.
      ; Take about 2G of this product powder (through No. 2 screen), weigh it accurately, place it in a corked conical flask, add 25ml of 85% methanol precisely, weigh it, heat and reflux for 1.5 hours, cool it, weigh it again, make up the lost weight with 85% methanol, shake it well, filter it, and take the filtrate.
      Assay   Precisely suck 5 μ l of the reference solution and 5 μ l of the test solution respectively, inject them into the liquid chromatograph, and determine.
      According to the calculation of thousand dry products, the content of luteolin (c15h10o6) shall not be less than 0.050%; .
      Decoction pieces
      [processing] remove impurities, wash, segment and dry.
      . The stem is cylindrical, ribbed, brown or dark brown on the surface, and covered with short hairs. The cut surface is yellowish white, hollow or with white pulp. The calyx is long tubular, yellowish brown to black brown, with obvious 10 longitudinal edges, and the apex is 5-lobed. The capsule is narrowly oval, slightly shorter than the calyx, brownish black, and the seeds are fine.
      [identification] (except for the transverse section of the stem), [inspection], [extract], [content determination] are the same as those of medicinal materials.
      [nature, taste and meridian tropism] bitter, cold. .
      [functions and indications] activating blood circulation and removing blood stasis, dredging meridians and relieving pain, cooling blood, stopping bleeding, clearing heat and removing dampness. It is used for traumatic injury, trauma hemorrhage, blood stasis, menstrual closure, irregular menstruation, postpartum blood stasis and pain, symptom accumulation, blood dysentery, blood drenching, damp heat and jaundice, edema and abdominal distension, and excessive leucorrhea.
      .
      [storage] put it in a dry place.


2、 Chemical constituents of Eupatorium odoratum
containArtemisia annua flavone（arteanoflavone），coumarin（coumarin），Isoeupatorin（eupatilin），Triterpene（tricin），Methoxycoumarin（herniarin），Scopoletin Pavilion（Scopoletin）， Umbelliferone,Salvianin（salvigenin），Chuannomonene lactone（reynosin）， Armexifolin, de hydromatricarin, deacetyl.

; Secotanapartholide a, tanaphillin  isomet, artanomaloide, arteanomalactone,Simi azalea（simiarenol），aurantiamide acetate （aurantiamide acetate）， ,palmitic acid（palmitic acid）， .

3、 Pharmacological effects of beiliujinu
Animal experiments show that this product can accelerate blood circulation, relieve smooth muscle spasm, and promote hemagglutination; The decoction can increase the perfusion flow of guinea pigs, and has obvious anti hypoxia effect on mouse hypoxia model. The decoction has inhibitory effect on Shigella sonnei and Shigella flexneri. [2]