Radix Aconiti kusnezoffii
1、 Pharmacopoeia standard of Radix Aconiti Preparata       This product is processed from Aconitum kusnezoffii.
      [preparation method] take Aconitum kusnezoffii, separate its size, soak it with water until there is no dry heart inside, take it out, add water and boil it until there is no white heart inside the cut, and there is a slight numb tongue feeling in the mouth, take it out, air it to 60% dry, cut it into thin pieces, and dry it.
      [properties] this product is irregular round or nearly triangular. The surface is dark brown, with gray white polygonal cambium rings and punctate vascular bundles, and there are gaps, and the periphery shrinks or bends. Brittle. .
      [identification] take 2G of this product powder, add 2ml of ammonia test solution to moisten, add 20ml of ether, sonicate for 30 minutes, filter, volatilize the filtrate, add 1ml of dichloromethane to dissolve the residue as the test solution. In addition, take benzoyl aconitine reference substance, benzoyl hypaconitine reference substance, benzoyl mesaconitine reference substance, and add isopropanol chloroform (1:1) mixed solution to make a mixed solution containing 1mg per 1ml as the control solution. According to the test of thin-layer chromatography (general rule 0502), suck 5 μ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use n-hexane ethyl acetate methanol (6.4:3.6:1) as the developing agent, put them in the developing cylinder saturated with ammonia vapor for 20 minutes, develop, take out, dry them, and spray dilute bismuth potassium iodide test solution. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
      ; Not more than 12.0% (the second method of general rule 0832).
      Diester alkaloids   Test according to the chromatographic conditions and the preparation method of the test solution under [content determination].
      Preparation of reference solution   Take an appropriate amount of aconitine reference substance, hypaconitine reference substance and mesaconitine reference substance, accurately weigh, add isopropanol chloroform (1:1) mixed solution to make a solution containing 30 μ g of aconitine, 10 μ g of hypaconitine and 50 μ g of mesaconitine per 1ml, respectively.
      ; Precisely suck 10 μ l of the reference solution and 10 μ l of the test solution under [content determination] respectively, inject them into the liquid chromatograph, and determine.
      .
      [content determination] determine according to HPLC (general rule 0512).
      ; Octadecylsilane bonded silica gel was used as filler; Acetonitrile tetrahydrofuran (25:15) was used as mobile phase a, and 0.1mol/l ammonium acetate solution (0.5ml of glacial acetic acid per 1000ml) was used as mobile phase B. gradient elution was performed as specified in the following table; The detection wavelength was 235nm. The number of theoretical plates should not be less than 2000 according to the peak of benzoyl mesaconitine.
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      Time (minutes)       ;     Mobile phase B (%)
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     0～48          15→26         85→74
    48～48.1        26→35         74→65
    48.1～58          35             65
    58～65          35→15         65→85
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      Preparation of reference solution   Take an appropriate amount of benzoyl aconitine reference substance, benzoyl hypaconitine reference substance and benzoyl mesaconitine reference substance, accurately weigh them, add isopropanol chloroform (1:1) mixed solution to make a mixed solution containing 20 μ g of benzoyl aconitine, 0.1mg of benzoyl mesaconitine and 80 μ g of benzoyl mesaconitine per 1ml, respectively.
      Preparation of test solution   Take about 2G of this product powder (passing through No. 3 screen), accurately weigh it, place it in a corked conical flask, add 3ml of ammonia test solution, precisely add 50ml of isopropanol ethyl acetate (1:1) mixed solution, weigh it, and sonicate it (power 300W, frequency 40KHz; Water temperature is below 25 ℃) for 30 minutes, cool, weigh again, use isopropanol ethyl acetate (1:1) mixed solution to make up the lost weight, shake well, and filter. Accurately measure 25ml of the secondary filtrate, recover the solvent to dryness under reduced pressure below 40 ℃, precisely add 3ml of isopropanol chloroform (1:1) mixed solution to dissolve the residue, filter, and take the secondary filtrate.
      Assay   Precisely suck 10 μ l of the reference solution and 10 μ l of the test solution respectively, inject them into the liquid chromatograph, and determine.
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      [nature, taste and meridian tropism] pungent, bitter and hot; . Homing, liver, kidney and spleen meridians.
      [function and indications] the same as Radix Aconiti kusnezoffii.
      [usage and dosage] 1.5 ~ 3G, it is advisable to decoct first and for a long time.
      .


2、
containAconitine（aconitine），Mesoaconitine（mesaconitine）、 Atisine et al.

3、 Pharmacological effects of Radix Aconiti Preparata
Expel wind and dampness, warm meridians and relieve pain. It is used for wind cold dampness arthralgia, joint pain, cold pain in the heart and abdomen, cold hernia pain, anesthesia and pain relief.