Pulsatilla chinensis
1、 Pharmacopoeia standard of Pulsatilla chinensis       This product is the dried root of Pulsatilla chinensis (bge.) Regel, a plant of Ranunculaceae. .
      [properties] this product is quasi cylindrical or conical, slightly twisted, 6-20cm long, 0.5-2cm in diameter. The surface is yellowish brown or tan, with irregular longitudinal wrinkles or grooves. The skin is easy to fall off, exposing the yellow wood. Some have network cracks or fissures, and there are often decayed pits near the root. The root head is slightly expanded, with white villi, and sheath like petiole residues can be seen in some. . It is slightly bitter and astringent.
      [identification] (1) the powder of this product is gray brown. The phloem fibers are spindle or spindle shaped, 100-390 µ m long, 16-42 µ m in diameter, and the walls are lignified. . Marginal pitted conduit, reticulated conduit and threaded conduit, with a diameter of 10-72 µ M.
      (2) Take 1g of this product, grind it into fine powder, add 10ml of methanol, sonicate for 10 minutes, filter it, and take the filtrate as the test solution. Another 1g of Pulsatilla reference medicinal material was taken, and the reference medicinal material solution was prepared by the same method. According to the test of thin-layer chromatography (general rule 0502), suck 5 µ l of each of the above two solutions, dot them on the same silica gel G thin-layer plate, use the upper solution of n-butanol acetic acid water (4:1:2) as the development mark, develop, take out, air dry, spray 10% sulfuric acid ethanol solution, heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots of the same color appear on the corresponding position of the chromatogram of the control medicinal material.
      [check] moisture   Not more than 13.0% (the second method of general rule 0832).
      Total ash   Not more than 11.0% (general rule 2302).
      Acid insoluble ash content shall not exceed 6.0% (general rule 2302).
      [extract] according to the cold leaching method under the alcohol soluble extract determination method (general rule 2201), the water saturated n-butanol as solvent shall not be less than 17.0%
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test   Octadecylsilane bonded silica gel was used as filler, and methanol water (64:36) was used as mobile phase; The detection wavelength is 201nm, and the number of theoretical plates should not be less than 3000 according to the peak of Pulsatilla Saponin B4.
      Preparation of reference solution   Take an appropriate amount of Pulsatilla Saponin B4 reference substance, accurately weigh it, add methanol to make a solution containing 0.1mg per 1ml.
      Preparation of test solution   Take 0.2g of this product powder (passing through No. 3 screen), accurately weigh it, put it into a corked conical flask, add 10ml of methanol, close the stopper, sonicate (power 150W, frequency 40KHz) for 25 minutes, cool it, filter it, put the filtrate into a 250ml volumetric flask, wash the container and residue with a small amount of mobile phase, merge the washing solution into the same volumetric flask, add mobile phase to the scale, shake it evenly, and then get it.
      Assay   .
      According to the calculation of dry product, the content of Pulsatilla Saponin B4 (c59h96o26) shall not be less than 4.6%.
      Decoction pieces
      [processing] remove impurities, wash and moisten thoroughly; Slice and dry.
      This product is shaped like a round piece. The skin is yellowish brown or tan in appearance, with irregular longitudinal wrinkles or grooves, and white villi near the root head. The cut skin is yellowish white or yellowish brown, and the wood is yellowish. It is slightly bitter and astringent.
      [identification], [inspection], [extract], [content determination] are the same as those of medicinal materials.
      [nature, taste and meridian tropism] bitter, cold. Return to stomach and large intestine.
      [functions and indications] it can clear away heat, detoxify, cool blood and stop dysentery. It is used for fever, blood dysentery, yin and itch.
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2、 Chemical constituents of Pulsatilla chinensis
Pulsatilla root containsPulchinenoside A3、Pulsatilla Saponin B、whiteTauweng saponinC、Pulsatilla SaponinD， 3-O - α - l-rhamnopyranosyl - (1 → 2) - α - l-arabinose-3 β, 23 dihydroxy - △ 20 (29) - lupinen-28-acid [3-O - α - l-rhamnopyranosyl - (1 → 2) α - l-arabinopyranosyl-3 β, 23 dihydroxylup △ -20 (29) - en-28-oic acid],Pulchinenoside A3、Pulsatilla SaponinB4, saponin 1 and 2, betulinicacid 3-O - α - l-arabinopyranoside,Betulinic acid（betulinicacid）， 3-oxobetulinicacid,Carotene（daucosterol）， Anemonin, protoanemonin.

The root of Pulsatilla chinensis contains the epidisaccharide saponin of Clematis chinensis (cp3a), the disaccharide saponin of Clematis chinensis (CP2), saponin II and saponin III.

The roots of pulsatiloside a, B, C, D, leontoside a, B, D, calcoside D, cauloside DAescin f。

3、
1. anti amoeba protozoa
2. anti Trichomonas vaginalis
3. antibacterial effect
4. antiviral effect