Wangbuliuxing
1、       This product is the dried and mature seed of the Dianthus plant Vaccaria segetalis (neck.) garcke. In summer, when the fruit is mature and the peel is not yet cracked, the plants are harvested, dried, seeded, removed of impurities, and then dried.
      [properties] the product is spherical, with a diameter of about 2mm. The surface is black, a few red brown, slightly shiny, with fine granular protrusions, and a concave longitudinal groove on one side. Hard quality. . The breath is slight, and the taste is slightly astringent and bitter.
      . The epidermal cells of seed coat are reddish brown or yellowish brown, polygonal or long polygonal on the surface, with a diameter of 50-12 μ m, thickened peripapillary wall, and star angular or deep wavy bending. The epidermal cells in the seed coat are yellowish brown, and appear square, rectangular or polygonal on the surface. The pericarp wall is thickened in a tight bead shape, and a reticular thickening texture can be seen on the surface. Endosperm cells are polygonal, square like or rectangular, and their cavities are filled with starch granules and aleurone granules. Cotyledon cells contain fatty oil droplets.
      (2) Take 1.5g of this product powder, add 20ml of methanol, heat and reflux for 30 minutes, cool, filter, evaporate the filtrate, add 2ml of methanol to dissolve the residue, and use it as the test solution. Another 1.5g of wangbuliuxing reference drug was taken and prepared into the reference drug solution by the same method. . In the chromatogram of the test sample, the same orange red spots appear at the corresponding positions of the chromatogram of the control medicinal material.
      (3) Take 1g of this product powder, add 40ml of 70% methanol, sonicate for 30 minutes, cool, filter, and the filtrate is used as the test solution. Another 1g of wangbuliuxing reference medicinal material was prepared into the reference medicinal material solution by the same method. Then take the reference substance of wangbuliuxing flavonoid glycoside and add methanol to make a solution containing 0.1mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 2 μ l of each of the above three solutions, dot them on the same polyamide film, use methanol water (4:6) as the developing agent, develop, take out, dry, spray 2% aluminum trichloride ethanol solution, dry with hot air, and view under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample.
      [check] moisture   .
      Total ash   Not more than 4.0% (general rule 2302).
      .
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test   Octadecylsilane bonded silica gel was used as filler; Use methanol as mobile phase A and 0.3% phosphoric acid solution as mobile phase B, and perform gradient elution according to the following table; The detection wavelength was 280nm. The number of theoretical plates should not be less than 3000 according to the flavonoid glycoside peak of Vaccaria.
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      Time (min) mobile phase a (%) mobile phase B (%)
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     0～10       35          65
    10～20      35→40      65→60
    20～35      40→50      60→50
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      Preparation of reference solution   Take an appropriate amount of wangbuliuxing flavonoid glycoside reference substance, accurately weigh it, add 70% methanol to make a solution containing 0.1mg per 1ml.
      Preparation of test solution   Take about 1.2g of this product powder (passing through No. 3 screen), weigh it accurately, place it in a corked conical flask, add 50ml of 70% methanol precisely, weigh it, sonicate (power 250W, frequency 33khz) for 30 minutes, cool it, weigh it again, make up the lost weight with 70% methanol, shake it well, filter it, and take the filtrate.
      Assay   Precisely suck 10 μ l of the reference solution and 10 μ l of the test solution respectively, inject them into the liquid chromatograph, and determine.
      According to the calculation of dry product, the content of xanthone glycoside (c32h38o19) in this product shall not be less than 0.40%.
      Decoction pieces
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      [character], [identification], [inspection], [exudate], [content determination] are the same as those of medicinal materials.
      Stir fried wangbuliuxing   Take wangbuliuxing, stir fry according to the clear frying method (general rule 0213) until most of the white flowers burst.
      .
      [check] moisture   .
      [content determination] the same as the medicinal material, the content of wangbuliuxing flavone glycoside (c32h38o19) shall not be less than 0.15%.
      [identification] (except for fine powder) [extract] is the same as that of medicinal materials.
      [nature, taste and meridian tropism] bitter, flat. Return to liver and stomach channels.
      [functions and indications] activating blood circulation and dredging menstruation, reducing swelling in the lower mammary gland, diuresis and gonorrhea. It is used for amenorrhea, dysmenorrhea, retention of milk, swelling and pain of breast carbuncle, astringency and pain of drenching syndrome.
      [usage and device] 5 ～ 10g.
      [note] pregnant women should use it with caution.
      [storage] put it in a dry place.

2、 Chemical constituents of Bulbus Vaccariae
The seeds contain triterpenoid saponins, known as VAC segoside, including a, B, C, and D, which are all polysaccharides derived from gypsogenin [1]. The structures of saponins B and C of Vaccaria have been determined [2, 3]. The sugar part of saponin A includes D-glucuronic acid, D-galactose, L-arabinose, D-xylose, D-fucose, L-rhamnose, and the sugar part of saponin D includes D-glucuronic acid, D-galactose, D-xylose, D-fucose, and D-glucose [1]. The same vacraoside was obtained from hydrolysis of the four saponins, namely gypsogenin-3 - β - d-giucuronoside [1]Vaccaria flavonoid glycoside（vaccarin）[4]，Isosaporin（isosaponarin）[5]。 It also contains phytin, phospholipid [6],Stigmasterol(stigmasterol) [7] et al.

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3、 Pharmacological effects of wangbuliuxing
The anti early pregnancy effect of 0.25% - 0.5% decoction has contractile effect on isolated rat uterus, and the effect of ethanol extract is stronger than that of Decoction (mixed miscellaneous four seed wild pea decoction has no effect). Thirty healthy female rats (weighing 25-30g) were randomly divided into three groups. According to the experimental method of implantation and anti pregnancy, the male rats were caged according to 2:1, and the alcohol extract of wangbuliuxing (according to the original Chinese medicine 0.05g/10g/ day) was given; One group was given normal saline as control, and male rats were caged according to 2:1; In addition, female rats with normal non spouse were taken as blank control. . The cAMP content was determined by radioimmunoassay. The results showed that the cAMP level in the normal group was consistent with that reported at home and abroad. For the normal pregnant mouse group, the cAMP content in plasma and uterine tissue increased significantly due to pregnancy. Compared with the normal mouse group, the difference between the groups was significant, with statistical significance (p< 0.05). The increase of cAMP content in plasma and uterine tissue in the anti implantation anti early pregnancy nonpregnant group was statistically significant compared with that in the normal mouse group (p< 0; ; 0.05); Compared with the normal pregnant mice group, the cAMP content in plasma and uterine tissue was also significantly increased, and the difference between the groups was significant, P< It is suggested that wangbuliuxing has anti implantation and anti early pregnancy effects on mice, and at the same time it can regulate physiological functions and affect metabolism in vivo, resulting in a significant increase in the second messenger substance (cAMP) in the plasma myometrium of mice.