Rhizoma Belamcandae
1、 Pharmacopoeia standard of Sichuan Shegan       . It can be dug all year round to remove fibrous roots and sediment and dry.
      [properties] the product is irregular strip or cone-shaped, slightly flat, branched, 3-10cm long, 1-2.5cm in diameter. The surface is grayish yellowish brown or brown, with ring and longitudinal grooves. There are often residual fibrous roots and concave or dot shaped fibrous root scars. It is brittle and easy to break, and the section is yellowish white or yellowish brown. It tastes sweet and bitter.
      [identification] (1) the powder of this product is light yellow. Calcium oxalate has many columnar crystals, most of which have been broken. The intact ones are 15 ～ 82 μ m long (up to 300 μ m) and 16 ～ 52 μ m in diameter. Parenchyma cells are round or oval in shape, with slightly thick or bead like walls and single striated pores. Cork cells are polygonal in appearance, thin in wall, microwave like bending, and some have brown objects.
      (2) Take LG of the product powder, add 10ml of methanol, sonicate for 30 minutes, filter, and concentrate the filtrate to about 1ml as the test solution. . Then take Belamcandin reference substance and add methanol to make a solution containing 0.5mg per 1ml as the reference solution. Test according to thin-layer chromatography (general rule 0502), suck 1 μ l of each of the above three solutions, dot them on the same polyamide film, use chloroform butanone methanol (3:1:1) as the developing agent, develop, take out, dry, spray aluminum trichloride test solution, and inspect under ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample.
      [inspection] the moisture content shall not exceed 15.0% (the second method of general rule 0832).
      The total ash content shall not exceed 7.0% (general rule 2302).
      [extract] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 24.0%.
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol-0.05mol/l potassium dihydrogen phosphate solution (pH adjusted to 3.0 with phosphoric acid) (32:68); The detection wavelength was 265nm. The number of theoretical plates should not be less than 2500 according to the Belamcandin peak.
      Preparation of reference solution take an appropriate amount of Belamcandin reference, weigh accurately, add 70% ethanol to make a solution containing 20 μ g per 1ml.
      Preparation of test solution take about 0.5g of powder (passing through No. 3 screen), accurately weigh it, place it in a corked conical flask, precisely add 25ml of 70% ethanol, close the stopper, weigh it, sonicate (power 250W, frequency 50KHz) for 1 hour, cool it, weigh it again, use 70% ethanol to make up the lost weight, shake it well, filter it, accurately measure 1ml of continuous filtrate, place it in a 50ml measuring flask, add 70% ethanol to dilute it to the scale, shake it well, and get it.
      The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      The content of Belamcandin (c22h24o11) in this product shall not be less than 3.6% calculated as dry product.
      Decoction pieces
      [processing] remove impurities, wash, moisten, slice, and dry.
      This product is irregular flake. The outer skin is grayish yellowish brown or brown, and sometimes there are ring stripes, or four pit or dot shaped fibrous root scars. The cut surface is yellowish white or yellowish brown. It tastes sweet and bitter.
      [inspection] the moisture content is the same as that of medicinal materials, and shall not exceed 13.0%.
      .
      [identification], [extract], [content determination] are the same as those of medicinal materials.
      [nature, taste and meridian tropism] bitter, cold. .
      [functions and indications] it can clear away heat, detoxify, expectorant, and promote swallowing. It is used for heat toxin, phlegm and fire stagnation, sore throat, phlegm and saliva congestion, cough and asthma.
      [usage and dosage] 6 ～ 10g.
      [storage] put it in a dry place.

2、 Chemical constituents of Rhizoma Belamcandae
  containIrisin, irisin, irisin, rhapontinApocynin, oleander diglycosideirisflorentinAnd 3 ', 5', 7 ', trihydroxy-4', 6 'dimethyl isoflavone. The ethanol extract has significant inhibitory effect and antipyretic effect on the early and late stages of inflammation, and has expectorant effect. Its flavonoids have anti allergic effects.

3、 Pharmacological effects of Rhizoma Belamcandae (Bl.) Merr.The pharmacological effects of Rhizoma Belamcandae (Bl.) Merr.The root of Rhizoma Belamcandae (Bl.) Merr..the root of Rhizoma Belamcandae
It has antipyretic, expectorant, anti-inflammatory and anti allergic effects; It has certain inhibitory effect on Staphylococcus aureus, Bacillus anthracis, Bacillus diphtheriae and Streptococcus B in vitro; In addition, it can induce vomiting and diarrhea. The ethanol extract has significant inhibitory effect and antipyretic effect on the early and late stages of inflammation, and has expectorant effect. Its flavonoids have anti allergic effects.