1、 Pharmacopoeia standard of plum blossom This product is the dried flower buds of Prunus mume (sieb.) sieb.et zucc., a Rosaceae plant. .
[character] this product is quasi spherical, with a diameter of 3-6mm and short stem. Bracts are several layers, scaly, brown. Calyx 5, grayish green or brownish red. . Stamens numerous; Pistil 1, ovary densely pilose. Light weight. The smell is light and fragrant, and the taste is slightly bitter and astringent.
[identification] (1) the powder of this product is brown. The pollen grains are nearly spherical, in polar view they are quasi circular triangles, 35-45 μ m in diameter, and 3-pore grooves. . Calcium oxalate crystals exist in parenchyma cells or are scattered, with a diameter of 8 ～ 33 μ m, and the edges and corners are not obvious or wide and blunt, and some are in fragments. The surface of the epidermal cells of bracts or sepals is square, rectangular or irregular polygonal, the vertical wall is slightly Beaded thickened, the cuticular texture is faintly visible, and the pores are visible. The inner wall cells of pollen sac have fine reticular thickened texture, which is rare.
(2) Take 0.5g of this product powder, add 15ml of 50% methanol, sonicate for 30 minutes, filter, and take the filtrate as the test solution. Take 0.5g of plum blossom control medicinal material and prepare the control medicinal material solution by the same method. Then take chlorogenic acid control sample and Isoquercitrin control sample, add methanol to make a mixed solution containing 50 μ g chlorogenic acid and 25 μ g Isoquercitrin per 1ml as the control solution. According to the test of thin-layer chromatography (general rule 0502), absorb 2 ～ 4 μ g of each of the above three solutions, respectively dot on the same polyamide film, use n-butanol acetic acid water (5:0.15:4) as the developing agent, develop, take out, dry, spray with 3% aluminum trichloride ethanol solution, heat with hot air until the spots are clear, and inspect under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 13.0% (the second method of general rule 0832).
The total ash content shall not exceed 10.0% (general rule 2302).
[extract] determined by the hot leaching method under the determination of alcohol soluble extract (general rule 2201), using dilute ethanol as solvent, it shall not be less than 30.0%.
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Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Acetonitrile containing 0.1% formic acid was used as mobile phase a, and 0.1% formic acid solution was used as mobile phase B. gradient elution was performed according to the following table; The detection wavelength was 355nm. The number of theoretical plates should not be less than 5000 according to hyperin peak.

Preparation of reference solution take an appropriate amount of chlorogenic acid reference, Hyperoside reference and Isoquercitrin reference, accurately weigh, add 50% methanol to make a mixed solution containing 0.2mg of chlorogenic acid, 15 μ g of Hyperoside and 15 μ g of Isoquercitrin per 1ml.
Preparation of test solution take about 0.5g of the powder (passing through No. 4 screen), weigh accurately, place in a corked conical flask, add 50ml of 50% methanol precisely, close the stopper, weigh, sonicate (power 250W, frequency 40KHz) for 45 minutes, cool, weigh again, use 50% methanol to make up for the lost weight, shake well, filter, and take the filtrate.
The determination method is to precisely suck 5 μ l each of the reference solution and the test solution, inject them into the liquid chromatograph, and determine.
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[nature, taste and meridian tropism] slightly sour, flat. It belongs to the liver, stomach and lung meridians.
[functions and indications] soothe the liver and moderate, dissipate phlegm and disperse nodules. It is used for liver and stomach pain, depression, plum core gas, and sores.
[usage and dosage] 3-5g.
[storage] store in a cool and dry place to prevent mold and moth.

2、 Chemical constituents of Prunus mume

Plum blossom contains volatile oil, which mainly contains benzaldehyde, benzyl alcohol, 4-terpineol,palmitic acid, benzoic acid, isosyringol, and other flavonoidsrutin、quercetin 、chlorogenic acidEtc.

3、 Pharmacological action of plum blossom

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