1、 Pharmacopoeia standard of Cuscuta chinensis
This product is the dried and mature seeds of the Convolvulaceae plant Cuscuta australis r.br. or Cuscuta chinensis lam. The plants were harvested when the fruits matured in autumn, dried in the sun, and seeded to remove impurities.
. It is hard to crush with nails. The air is weak and the taste is light.
[identification] (1) take a small amount of this product and soak it in boiling water. The surface is sticky; When heated and boiled until the seed coat is broken, the yellow and white spiral shaped embryo can be exposed, which is like spinning.
(2) The powder of this product is yellowish brown or dark brown. The cross-section of seed coat epidermal cells was square or rectangular, and the lateral wall was thickened; The surface is round and polygonal, and the wall at the corner is significantly thickened. The seed coat grid cells were in sheets, and the cross-sectional view showed 2 rows. The outer row cells were shorter than the inner row cells, with a brilliant band, located in the upper part of the inner row cells; The surface is polygonal and wrinkled. . The cotyledon cells showed vague powder particles and fatty oil droplets.
(3) Take 0.5g of this product powder, add 40ml of methanol, heat and reflux for 30 minutes, filter, and concentrate the filtrate to 5ml as the test solution. Take 0.5g of Cuscuta chinensis reference material and make the reference material solution by the same method. Then take hyperin reference substance and add methanol to make a solution containing 1mg per 1ml as the reference substance solution. According to the test of thin layer chromatography (general rule 0502), absorb 1 ～ 2 μ l of each of the above three solutions, respectively dot on the same polyamide film, use methanol glacial acetic acid water (4:1:5) as the developing agent, develop, take out, dry, spray aluminum trichloride test solution, and inspect under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 10.0% (the second method of general rule 0832).
The total ash content shall not exceed 10.0% (general rule 2302).
Acid insoluble ash content shall not exceed 4.0% (general rule 2302).
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Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-0.1% phosphoric acid solution (17:83); .
Preparation of reference solution take a proper amount of hyperin reference, weigh accurately, and add methanol to make a solution containing 48 μ g per 1ml.
Preparation of test solution take 1g of powder (passing through No. 4 screen), accurately weigh it, put it into a 50ml volumetric flask, add 40ml of 80% methanol, sonicate (power 500W, frequency 40KHz) for 1 hour, cool it, add 80% methanol to the scale, shake it well, filter it, and take the continuous filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
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Decoction pieces
[processing] dodder seed is cleaned and dried to remove impurities.
[character], [identification], [inspection], [content determination] are the same as those of medicinal materials.
Take the salt dodder and stir fry it according to the salt roasting method (general rule 0213) until it is slightly swollen.
This product is shaped like dodder, with brown yellow surface, cracking, and slight aroma.
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. It belongs to the liver, kidney and spleen meridians.
; For external use, it can eliminate wind and freckle. It is used for liver and kidney deficiency, waist and knee soreness, impotence and spermatorrhea, enuresis and frequent urination, kidney deficiency and fetal leakage, restless fetal movement, dizzy eyes and tinnitus, spleen and kidney deficiency diarrhea; External treatment of vitiligo.
[usage and dosage] 6 ～ 12g. Suitable for external use.
[storage] store in a ventilated and dry place.

2、 Chemical constituents of Cuscuta chinensis

Cuscuta seed containsquercetin (quercetin)，Astragaloside(astragalin)，Hyperin(hyperin) and quercetin-3-o - β - d-galactoside-7-o - β -glucoside. Alkaloids in the fruit of Cuscuta chinensis var. australis.

3、 Pharmacological effects of Cuscuta chinensis

1. liver protective effect: dodder 20% water decoction is given to mice injured by carbon tetrachloride by gavage, and 50g crude drug /kg body weight can reduce the increased lactic acid, pyruvate and SGPT in the blood, and increase the decreased hepatic glycogen and adrenal ascorbic acid, which has significant protective activity against liver injury.

2. Yang boosting and sexual vitality enhancing effects: 20% Cuscuta chinensis Decoction 0.5ml/ mouse / day by gavage can restore the symptoms of Yang deficient mice. .

3. increase non-specific resistance: dodder water decoction can prolong the weight-bearing swimming time of mice, enhance the hypoxia tolerance of mice under normal pressure, and improve their non-specific resistance.

4. other effects: Cuscuta chinensis has anti-tumor, anti-virus, anti-inflammatory, anti infertility, diarrhea causing and central nervous system inhibiting effects.