1、 Pharmacopoeia standard of Radix Bupleuri
This product is the dried root of Stellaria dichotoma L.var. lanceolata bge., a plant of Dianthus family. Excavate when plants germinate in spring and summer or stem and leaf wither after autumn; The cultivated products shall be dug in the middle of September in the third year or April in the fourth year after planting, and the residual stems, fibrous roots and silt shall be removed and dried in the sun.
[properties] this product is cylindrical, occasionally branched, 15-40cm long, 0.5-2.5cm in diameter. The surface is light brown yellow to light brown, with twisted longitudinal wrinkles and root marks, and many holes or disc-shaped depressions, commonly known as "sand holes". Breaking from the sand holes shows that fine sand is scattered in the Brown cracks. The root head is slightly expanded, with dense verrucous buds, stems or residues of rhizomes, which is often called "Pearl disc". It is hard and brittle, easy to break, with uneven section, loose, cracks, thin skin, and yellow and white radial texture on the wood. The air is weak and the taste is sweet.
. There are few sand holes. There are many warty protrusions on the root head. The texture of the broken surface is relatively tight, with few cracks, slightly powdery, and the radial texture of the wood is not very obvious. It tastes slightly sweet.
[identification] (1) cross section of this product: the number of cork cells is more than 10. The inner layer of the bolt is narrow. The phloem sieve tube group was obvious. The cambium forms a ring. . The rays are as wide as more than 10 rows of cells. Parenchyma cells contain calcium oxalate sand crystals, which are more common in ray cells.
(2) Take 1g of this product powder, add 10ml of absolute ethanol, soak for 15 minutes, and filter. Take 2ml of the filtrate and put it under the UV lamp (365nm) to observe, showing blue and purple fluorescence.
(3) Take 0.1g of this product powder, add 25ml of methanol, sonicate for 10 minutes, filter, put the filtrate into a 50ml volumetric flask, and add methanol to the scale. According to the ultraviolet visible spectrophotometry (general rule 0401), there is a maximum absorption at the wavelength of 270nm.
[inspection] the acid insoluble ash content shall not exceed 5.0% (general rule 2302).
[extract] according to the cold leaching method under the determination method of alcohol soluble extract (general rule 2201), using methanol as solvent, it shall not be less than 20.0%.
Decoction pieces
[processing] remove impurities, wash, moisten, cut into thick pieces, and dry.
[nature, taste and meridian tropism] sweet, slightly cold. Return to liver and stomach channels.
[functions and indications] clear deficiency heat and eliminate malnutrition heat. For Ming deficiency fever, bone steaming fever, infantile malnutrition fever.
[usage and dosage] 3 ～ 10g.
[storage] store in a ventilated and dry place to prevent moths.

2、 Chemical constituents of Radix Bupleuri

Silver bupleurum root containsSpinasterol(α-spinasterol), ,Stigmasterol(stigmasterol), α - spinasterol glucoside, 7-stigmasterol glucoside,β - sitosterol（β-sitosterol）。

3、 Pharmacological effects of Radix Bupleuri

Gypsozid, a triterpenoid saponin extracted from Gypsophila Pacifica kom., a plant of the same genus, can be taken orally to rabbits at the same time of or after the formation of atherosclerosis, which can reduce the serum cholesterol concentration, reduce the cholesterol / cephalin coefficient, and reduce the aortic lipid content. The symptoms of excitement, depilation and subcutaneous lipid like thickening of limbs in rabbits with arteriosclerosis were improved. .