1、 Herbal classic standard of stinking pill
This product is the dry aboveground part of the Compositae plant Laggera pterodonta (dc.) benth. In autumn, when the stems and leaves are luxuriant, they are cut and dried.
[character] the product is 50 ~ 150cm long and is densely covered with yellowish glandular hair and pilose hair. The stem is cylindrical, with 4-6 longitudinal wings, and the wing margin is serrated, which is easy to break. Leaves alternate, with short stalks; . The capitulum is inserted at the end of the branch. Qi is peculiar and tastes bitter.
[identification] take 3G of this product powder, add 50ml of methanol, heat and reflux for 30 minutes, filter, evaporate the filtrate, add 2ml of methanol to dissolve the residue as the test solution. In addition, take the reference substance of artemisinin and add methanol to make a solution containing 1mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 5 μ l of each of the above two solutions, dot them on the same silica gel gf254 thin-layer plate, use dichloromethane ethyl formate acetone (6:0.5:0.3) as the developing agent, develop, take out, dry, and view under the UV light (254nm). In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 13.0% (the fourth method of general rule 0832).
The total ash content shall not exceed 12.0%. (general rule 2302)
Acid insoluble ash content shall not exceed 2.0%. (general rule 2302).
[extract] according to the hot leaching method under the determination method of water-soluble extract (general rule 2201), it shall not be less than 12.0%.
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Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-2% formic acid solution (35:65); The detection wavelength is 350nm. The number of theoretical plates should not be less than 4000 according to the peak of artemisinin.
Preparation of reference solution take an appropriate amount of the reference substance of artemisinin, weigh it accurately, add methanol to make a solution containing 35 μ g per 1ml.
Preparation of test solution take about 1.5g of powder (passing through No. 3 screen), weigh accurately, place in a corked conical flask, add 50ml of methanol precisely, close the stopper, weigh, heat and reflux for 1 hour, cool, weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of artemisinin (c20h20o8) shall not be less than 0.10%.
[nature, taste and meridian tropism] pungent, bitter and cold; . Return to lung meridian.
[functions and indications] heat clearing and detoxification, cough relieving and expectorant. It is used for wind heat, cold, sore throat, lung heat and cough.
[usage and dosage] 9 ～ 15g.
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2、 Chemical constituents of Eupatorium odoratum

Diol, aspartic acid,Artemisinin ,Psoralen B , 3,5-dihydroxy-6,7,3 ′, 4 ′ - tetramethoxyflavone, formonol, 5,6,4 ′ - trihydroxy-3,7-dimethoxyflavone,quercetin , tamarin, marigold, quercetin-3-o - β - D-galactopyranoside, marigold-3-o - β - d-glucopyranoside, quercetin-3-o - (6-p-coumaroyl) - β - d-glucopyranoside, 4 ', 5,7-trihydroxy-6-methoxyflavone-3-o - β - d-rutinoside, kaempferol-3-o - β - d-glucopyranosideStigmasterol , stigmasterol-3-o - β - d-glucopyranoside, o-hydroxybenzoic acidβ - sitosterol。

3、 Pharmacological effects of stinking pill

1. expectorant effect

Rabbits inhaled 0.9% ammonia water for 2 hours to produce acute inflammation of the upper respiratory tract, and oral administration of smelling pill liquid (first extract its volatile oil, and then make the drug residue into a decoction, and add the volatile oil into the decoction, containing 5g of crude drug per ml) 3 ml / kg; . It may be that part of the volatile oil contained in this product is excreted by the respiratory mucosa, which has mild stimulation on it, improves local blood circulation, promotes the healing of inflammation, and reduces excessive sputum volume.

2. treatment of experimental acute bronchitis

; If the animals were given 10 ml / kg of smelling pill orally, once every 1.5 hours for two times in total, the animals would be delayed to death within 12 hours.

3. anti tumor effect

Methylene blue decolorization method was used to determine the activity of blood cell dehydrogenase in leukemia patients in vitro. The concentrated ethanol extract of stinking pill decoction had a strong inhibitory effect on blood cell dehydrogenase in patients with acute lymphocytic leukemia, acute myeloid leukemia and acute monocytic leukemia. It can also significantly inhibit the respiration of leukocytes in patients with acute lymphoblastic leukemia (WAB respirator assay).

4. other functions

It has no antipyretic effect on rabbits with artificial fever, and no inhibitory effect on pneumococcus, Staphylococcus and Streptococcus B.