1、 Pharmacopoeia standard of Stellera chamaejasme
This product is the dried root of Euphorbia ebracteolata Hayata or Euphorbia fischeriana steud. .
[properties] Euphorbia officinalis is a quasi circular or oblong piece, with a diameter of 1.5 ~ 8cm and a thickness of 0.3 ~ 4cm. The outer skin is thin, yellowish brown or grayish brown, and is easy to peel off and expose the yellow skin. . It is light, brittle, easy to break, and has powdery cross-section. The air is weak and the taste is pungent.
The outer skin of Euphorbia chamaejasme is brownish yellow, and the cut texture or ring pattern is dark brown. There is stickiness after water immersion, and the stickiness can be seen when tearing.
[identification] (1) Euphorbia officinalis powder is yellowish white. There are many starch grains, single spherical, oblong or semi-circular, with a diameter of 3-34 μ m, umbilical fissure, herringbone or star shaped, and large grain lamina is faintly visible; The compound granules are composed of 2-5 granules; Semi compound particles are easy to see. The reticular marginal foramen duct was 18 ～ 80 μ M. The nipple tubes without knots are often broken, and the oil droplet like secretions contained are scattered; . Euphorbia Euphorbia powder is yellowish brown. The diameter of single starch granule is up to 24 μ m, and the compound granule is composed of 2-7 granules, and half compound granule is rare. The reticular marginated foramen duct was 102 μ m, and the milk was colorless.
(2) Take 2G of crude powder of this product, add 30ml of ethanol, heat and reflux for 1 hour, cool it, filter it, evaporate the filtrate to dryness, and add 2ml of methanol to dissolve the residue as the test solution. Another 2G of Stellera chamaejasme reference medicinal material was prepared into the reference medicinal material solution by the same method. According to the test of thin layer chromatography (general rule 0502), suck 2 μ l of each of the above two solutions, dot them on the same silica gel G thin layer plate respectively, use cyclohexane ethyl acetate (8.5:1.5) as the developing agent, develop, take out, air dry, spray 10% sulfuric acid ethanol solution, heat at 105 ℃ until the spots are clear, and check under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the position corresponding to the chromatogram of the control medicinal material.
[inspection] impurities shall not exceed 2% (general rule 2301).
The moisture content shall not exceed 13.0% (the second method of general rule 0832).
The total ash content shall not exceed 9.0% (general rule 2302).
Acid insoluble ash content shall not exceed 4.0% (general rule 2302).
[extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), using dilute ethanol as solvent, it shall not be less than 18.0%.
Decoction pieces
[processing] raw Stellera chamaejasme is used to remove impurities, wash, moisten, slice and dry.
Vinegar Stellera chamaejasme extract Stellera chamaejasme tablets and fry them according to the vinegar method (general rule 0213).
. The color is slightly dark, and the smell is slightly vinegar aroma.
[inspection] the total ash content is the same as that of medicinal materials, and shall not exceed 7.0%.
The acid insoluble ash content is the same as that of medicinal materials, and shall not exceed 1.0%.
[extract] the same as that of medicinal materials, not less than 20.0%.
[identification] [inspection] (moisture) is the same as that of medicinal materials.
; Toxic. Return to the liver and spleen meridians.
[function and indications] disperse knot and kill insects. External use for lymph node tuberculosis and dermatosis; Kill maggots.
[usage and dosage] boil cream for external application.
[note] it is not suitable to use with Buddha.
[storage] store in a ventilated and dry place to prevent moths.

2、 Chemical constituents of Stellera chamaejasme

. Diterpenoids: genidymuren, Heshuo manganin,Stellera chamaejasmeA、Stellera chamaejasmeB， 18 de - (benzoyloxy) - 28 deoxygenidimoren, 12 acetoxyheretoxin, ochratoxin. Flavonoids: stelleran achamaejasminB、Wolf toxinC， Wolf toxin, isowolf toxin（isochamaejasmin）, 7-methoxywolf toxin, Neowolf toxin AneochamaejasminB, chamaejasme chromone（chamaechromone）AndDihydrokaempferol,Pinoresinol, podocarpol（matairesinol）. Volatile oil: there are 27 components, 12 of which have been identified, mainly 3,7,17-trimethyldodecane-trans-2, cis-6,10-trienoic acid, 10,13-octadecadienoic acid methyl ester, n-tridecane, n-dodecane, 2,6-dimethylheptane andCinnamyl alcoholEt al. Fennel coumarin，Isobergamot lactone，Isoanisolactone，Saposhnikol.

3、

1. anti tumor: the water extract of Stellera chamaejasme L. can inhibit liver cancer and Lewis lung cancer in mice by intraperitoneal injection of 10-40g (crude drug) /kg and 2.5-20g/kg alcohol extract for 10 consecutive days; The water extract 10, 20g (crude drug) /kg, and the alcohol extract 2.5, 5.0g/kg were intravenously injected for 6 days, which also had inhibitory effects on liver cancer in mice; The water extract 69.5g/kg (crude drug) /kg and the alcohol extract 26.09g/kg were gavaged for 10 days, which also had an inhibitory effect on liver cancer in mice.

2. toxicity: the median lethal dose of Stellera chamaejasme water extract and alcohol extract by intraperitoneal injection to mice is 275.9g (crude drug) /kg and 171.96g (crude drug) /kg, respectively.