1、 Pharmacopoeia standard of semen Siegesbeckiae
This product is the dried and mature fruit of Leonurus japonicus houtt., a Labiatae plant. When the fruit is ripe in autumn, the aboveground part is cut, dried in the sun, and the fruit is laid to remove impurities.
[properties] this product is triangular, 2-3mm long and 1.5mm wide. Its surface is grayish brown to grayish brown, with dark spots. One end is slightly wide, flat, and the other end is gradually narrow and blunt. The pericarp is thin, and the cotyledons are white and oily. The breath is slight and the taste is bitter.
[identification] (1) the powder of this product is yellowish brown to dark brown. The exocarp cells were slightly radially elongated in cross-section and varied in length, forming mostly raised ridges, with yellow reticulated cells in the center of the ridge and non lignified walls; On the surface, it is polygonal, with strip keratinocyte texture, and reticulate cells with strip thickened wall. The section of thick walled cells in endocarp is slightly tangential extended, the inner wall is extremely thick, the outer wall is thin, and the cell cavity is lateral, containing calcium oxalate crystals; . Mesocarp cells are polygonal in appearance, with thin walls and fine wavy bends. The seed coat epidermal cells are square like, with slightly thick walls and slightly wavy bends, and the cell cavity contains yellowish brown substances. Endosperm cells contain fatty oil droplets and aleurone granules.
(2) Take 3G of the product powder, add 30ml of ethanol, heat and reflux for 1 hour, cool it, filter it, concentrate the filtrate to about 5ml, add it to the activated carbon alumina column (0.5g of activated carbon; 100-120 mesh of neutral alumina, 2G; inner diameter 10mm), elute with 30ml of ethanol, collect the eluent, evaporate it to dryness, add 0.5ml of ethanol to the residue to dissolve it, and use it as the test solution. In addition, take stachydrine hydrochloride reference substance and add ethanol to make a solution containing 5mg per 1ml as the reference substance solution. Test according to thin-layer chromatography (general rule 0502), suck 10 μ l each of the above two solutions, dot them on the same silica gel G thin-layer plate, use n-butanol hydrochloric acid water (4:1:0.5) as the developing agent, develop, take out, dry, and spray with dilute bismuth potassium iodide test solution. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 7.0% (the second method of general rule 0832).
The total ash content shall not exceed 10.0% (general rule 2302).
[extract] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 17.0%.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test strong cation exchange (SCX) column; The mobile phase was 15mmol/l potassium dihydrogen phosphate solution (containing 0.06% triethylamine and 0.14% phosphoric acid); The detection wavelength was 192nm. The number of theoretical plates should not be less than 3000 according to the stachydrine hydrochloride peak.
Preparation of reference solution take an appropriate amount of stachydrine hydrochloride reference, weigh accurately, add mobile phase to make a solution containing 40 μ g per 1ml.
Preparation of test solution take about 1g of powder (passing through No. 3 screen), accurately weigh it, place it in a corked conical flask, precisely add 25ml of ethanol, close the stopper, weigh it, heat and reflux for 1.5 hours, cool it, then weigh it again, use ethanol to make up the lost weight, shake it well, filter it, accurately measure 5ml of continuous filtrate, add it to a neutral alumina column (100-200 mesh, 3G, inner diameter of 1cm, wet packed column, pre washed with ethanol), elute with 100ml of ethanol, collect the eluent, recover the solvent to dryness, dissolve the residue with mobile phase, transfer it to a 5ml volumetric flask, dilute it to the scale, shake it well, and after filtration, take the continuous filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of stachydrine hydrochloride (c7h13no2 • HCl) shall not be less than 0.050%.
Decoction pieces
[processing] stir fry the semen salicis, take the purified semen salicis, and stir fry according to the clear frying method (general rule 0213) until there is a noise.
[nature, taste and meridian tropism] pungent, bitter and slightly cold. Return to pericardium and liver meridian.
[functions and indications] activating blood circulation, regulating menstruation, clearing liver and improving eyesight. It is used for irregular menstruation, amenorrhea, dysmenorrhea, erythema and pannus, dizziness and pain.
[usage and dosage] 5 ～ 10g.
[note] caution for those with dilated pupils.
[storage] store in a ventilated and dry place.

2、

Containing leonurinine,StachydrineThe main components of the oil are oleic acid (63.75%) and linolenic acid (21.13%), and vitamin A-like substances (0.04%).

3、 Pharmacological effects of semen Siegesbeckiae

1. hypotensive effect the aqueous extract or alcohol water extract of semen Artemisiae has a slight hypotensive effect on the anesthetic arteriovenous injection.

2. toxic people who take more than 30g of semen typhonii orally at a time can have toxic reactions 4-6h later. The symptoms are total radiation weakness, lower limbs can not move, the body aches and pains, and the severe cases are mostly in a collapsed state of sweating.