1、 Honglian pharmacopoeia standard
This product is a traditional Tibetan medicine. It is the dried whole grass of Lagotis brevituba maxim., a Scrophulariaceae plant. .
[character] the product is 5 ～ 15cm long. The rhizome is cylindrical, slightly curved, and closely spaced, resembling a silkworm; The surface is grayish brown or light purple brown; It is brittle and easy to break. The section is brown or grayish yellow. There are 3-4 white punctate vascular bundles arranged in a ring. The root is slender, cylindrical, twisted, with light yellowish brown or grayish brown surface and longitudinal wrinkles. Basal leaves, long petiolate; The leaves are mostly curly and broken, and the intact ones are round or ovoid after flattening, with blunt apex, round teeth at the edge, and wide wedge at the base. Spikes terminal. . The breath is slight, and the taste is slightly bitter.
. The starch grains are numerous, single round, 3-7 μ m in diameter, occasionally helmet shaped, umbilical punctate; Compound granules are composed of 2-3 (6) fractions. Parenchyma cells are round or quasi round, containing light brown quasi round nuclei. The lower epidermal cell wall of the leaf is slightly curved, and the stomatal infinity and inequality. The conduits are mostly reticulated conduits and threaded conduits.
(2) Take 0.5g of this product powder, add 10ml of methanol, sonicate for 15 minutes, filter, and use the filtrate as the test solution. In addition, take echinacoside control sample and verbascoside control sample, add methanol to make 1mg solution per 1ml as the control solution. Test according to thin-layer chromatography (general rule 0502), suck up 2 μ l of each of the above three solutions, dot them on the same polyamide thin-layer plate respectively, use methanol acetic acid water (2:1:7) as the developing agent, develop, take out, dry, and view under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the position corresponding to the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 8.0% (the second method of general rule 0832).
The total ash content shall not exceed 15.0% (general rule 2302).
Acid insoluble ash content shall not exceed 10.0% (general rule 2302).
[extract] according to the cold leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 8.0%.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Acetonitrile methanol 1% acetic acid solution (10:15:75) was used as the mobile phase; The detection wavelength was 334nm. The number of theoretical plates should not be less than 4000 according to the peak of echinacoside.
Preparation of reference solution take an appropriate amount of echinacoside reference substance, accurately weigh it, place it in a brown volumetric flask, add mobile phase to make a solution containing 0.25mg per 1ml.
Preparation of test solution take about 0.5g of powder (passing through No. 4 screen), weigh accurately, place in a 50ml Brown volumetric flask, add 25ml of mobile phase precisely, weigh, soak for 30 minutes, sonicate (power 230W, frequency 35kHz) for 15 minutes, cool, weigh again, use the mobile phase to make up for the lost weight, shake well, centrifuge, stand still, take the supernatant and place in a brown bottle.
The determination method is to precisely suck 10 ～ 20 μ l of the reference solution and the test solution respectively, inject them into the liquid chromatograph, and determine.
Calculated as a dry product, the content of echinacoside (c35h46o20) shall not be less than 0.80%.
[nature, taste and meridian tropism] bitter, sweet and cold. Return to lung, heart and liver channels.
[functions and indications] heat clearing, detoxification, dampness removing, liver calming, blood circulation and menstruation regulating. It is used for fever, thirst, lung heat, cough, headache, dizziness, damp heat, jaundice, irregular menstruation, and drug and food poisoning.
[usage and dosage] 1 ～ 6G.
[storage] store in a ventilated and dry place.

2、 Chemical constituents of Honglian

containLuteolin，Apigenin, liuchuanyusu, Eupatorium lutein,Morin，quercetin ，rutinAnd Medicago sativa, et al.

3、 Pharmacological action of Honglian

1. anti ulcer effect: the water extract (I) of this product (4g/kg) was administered by gavage on the first day after the chemical injury gastric ulcer was caused by glacial acetic acid in anesthetized rats. The drug was used for 7 consecutive days. The animals were sacrificed on the eighth day. The ulcer area and ulcer volume were taken as indicators. The results showed that I could significantly promote the healing of chronic gastric ulcer in rats and had anti ulcer effect.

2. sedative effect: 15g/kg was injected intraperitoneally. The spontaneous activity of mice began to decrease 10 minutes after administration. After 20 minutes, the mice crouched down, most of them closed their eyes and rested. Only individual animals showed slight activity, while the activity of animals in the control group did not decrease. 5g/kg intraperitoneal injection can significantly prolong the sleep time of pentobarbital, indicating that this product has a good sedative effect.