1、 Pharmacopoeia standard of fried Fructus Trichosanthis


Fried Fructus Trichosanthis
Chaogualouzi
TRICHOSANTHIS SEMEN TOSTUM
This product is processed from Fructus Trichosanthis
[preparation] take the Fructus Trichosanthis, stir fry it according to the general rule 0213, stir fry it gently until it is slightly swollen, take it out, and let it cool.
[properties] the product is flat and oval, 12-15mtn long, 6-10mm wide, and about 3.5mm thick. The surface is light brown to tan, smooth, occasionally with focal spots, a circle of grooves along the edge, a sharp top, a navel, and a blunt round or narrow base. ; Endoderm membranous, grayish green, cotyledon 2, yellowish white, oily. It smells slightly burnt and tastes light.
[identification] take 1g of this product powder, put it into a corked conical bottle, add 10ml of petroleum wine (60 ～ 90 ℃), sonicate for 10 minutes, filter, and take the filtrate as the test solution. Test according to thin-layer chromatography (general rule 0502), suck 10 µ 1 of the above test solution and 10 µ 1 of the control solution under the item of [content determination], respectively dot on the same silica gel G thin-layer plate, use cyclohexane ethyl acetate (5:1) as the developing agent, develop, take out, dry, spray 10% thioic acid ethanol solution, heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 10.0% (the second method of general rule 0832)
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Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Methanol water (93:7) was used as mobile phase; The detection wavelength was 230nm. The number of theoretical plates should not be less than 2000 according to the peak of 3,29-dibenzoyl-tritiol.
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Preparation of test solution take about 1g of the crude powder of the product (dried at 40 ℃ for 6 hours), accurately weigh it, put it into a 50ml conical flask with a stopper, precisely add 10ml of dichloromethane, close the stopper, weigh it, sonicate (power 250W, frequency 40KHz) for 30 minutes, cool it, weigh it again, make up the lost weight with dichloromethane, shake it well, stand still, and take the supernatant.
The determination method is to precisely suck 5 µ l each of the reference solution and the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of 3,29-dibenzoyl paclitaxel (c44h58o5) shall not be less than 0.060%.
[nature, taste and meridian tropism] sweet, cold. .
[functions and indications] moisten the lungs, remove phlegm, smooth the intestines and relieve constipation. .
[usage and dosage] 9 ～ 15g.
[note] it should not be used with Aconitum carmichaeli, Radix Aconiti carmichaeli, Radix Aconiti carmichaeli and aconite.
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2、 Chemical constituents of fried Fructus Trichosanthis

Fructus Trichosanthis mainly contains oils, sterols, amino acids, proteins, volatile oils and other chemical components

3、 Pharmacological effects of fried Fructus Trichosanthis

1. Anticancer effect Trichosanthes kirilowii seed has anticancer effect in vitro. Trichosanthes kirilowii glycoprotein extracted from Trichosanthes kirilowii seeds can inactivate ribosomes. Trichosanthes kirilowii glycoprotein conjugated monoclonal antibody (Tyl, 2 antigen) can selectively kill leukemia cells expressing Tyl, 2 antigen; The mechanism of Trichosanthes kirilowii glycoprotein immunotoxin entering cells is different from ricin A chain immunotoxin, and the former has a better application prospect in vivo.

2. Toxic oral overdose of Trichosanthes kirilowii can cause stomach discomfort, nausea, vomiting and abdominal pain diarrhea.

3. Purgative effect the fatty oil contained in Trichosanthes kirilowii kernel has strong purgative effect.

4. Other effects Trichosanthes kirilowii and kernel have the effect of dilating the coronary artery of isolated guinea pig heart.

5. Trichomonic acid, the main component of Trichosanthes kirilowii seed, can inhibit platelet aggregation stimulated by collagen, adenosine diphosphate and epinephrine in a concentration dependent manner, and the inhibitory potency is roughly the same as that of linolenic acid (LNA).