Tip at both ends
1、 Pharmacopoeia standard of Liangtoujian         This product is the dried rhizome of anemcme raddeana Regel, a Ranunculaceae plant. Excavate in summer, remove fibrous roots, wash and dry.
        [character]   This product is in the shape of a long spindle, with both ends pointed and slightly curved, and the near end is relatively expanded, with a length of 1 ~ 3cm and a diameter of 2 ~ 7mm. The surface is brownish brown to brownish black, with fine longitudinal wrinkles. There are often 1 ~ 3 branch root marks in the expanded part, which are fish fin like protrusions, and 3 ~ 5 links that are not obvious are occasionally seen. It is hard and brittle, easy to break, with a slightly flat section, white or grayish brown, slightly horny. The flavor is light at first and then bitter and spicy.
        [identification] (1) cross section of this product: 1 column of epidermal cells, tangential extension, thickening of the outer wall. . The outer vascular bundle is tough, with more than 10 arranged in rings, phloem cells shrunk, xylem vessels 6-24, and the cambium is not obvious. The rays are broad and the medulla is large, which is composed of round like parenchyma cells. Parenchyma cells were filled with starch granules.
        . The starch grains are numerous, single grain is round or oval, with a diameter of 2-11 μ m, umbilical punctate or short suture, and the laminar pattern is not obvious; Compound granules are composed of 2-4 fractions. The epidermal cells are reddish brown, yellow or bright yellow, and the outer wall is corky and thickened, often protruding into the cells in the form of ridges or tumors. Reticulated conduit, threaded conduit or ladder shaped conduit are common, with a diameter of 10-33 μ m, and few conduits with marginal pits.
        (2) Take the reference substance of Rhizoma Cyperus A and add methanol to make a solution containing 1mg per 1ml as the reference solution. Test according to thin-layer chromatography (general rule 0502), suck 2 μ l each of the test solution and the above reference solution under the item of [content determination], dot them on the same silica gel G thin-layer plate, use the lower layer solution of chloroform methanol water (7:3:1) as the developing agent, develop, take out, air dry, spray 10% sulfuric acid ethanol solution, and heat at 105 ℃ for 5 minutes. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
        [check]   .
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        ; According to the hot leaching method under the determination of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 12.0%.
        [content determination]   Determine according to high-performance liquid chromatography (general rule 0512).
        Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler, acetonitrile as mobile phase a, and 0.1% phosphoric acid solution as mobile phase B, and perform gradient elution according to the following table; The detection wavelength was 206nm. The number of theoretical plates should not be less than 4000 according to the peak of Rhizoma Cyperus a.
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      0～7           47           53
      7～15         47→55         53→45
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        Preparation of reference solution take an appropriate amount of the reference substance of Rhizoma Cyperus a, weigh it accurately, and add methanol to make a solution containing 1mg per 1ml.
        Preparation of test solution take about 5g of powder (passing through No. 3 screen), weigh accurately, put it into Soxhlet extractor, add appropriate amount of methanol, heat and reflux for extraction for 3 hours, recover the solvent from the extraction solution to dryness, add 10ml of water to dissolve the residue, shake with diethyl ether for two times (20ml, 10ml), and discard the diethyl ether solution. The water solution was extracted with saturated n-butanol by shaking for five times (20ml, 20ml, 15ml, 15ml, 15ml). The n-butanol solution was combined, and the solvent was recovered to dryness under reduced pressure. .
        The determination method is to precisely suck the reference solution and the test solution into the liquid chromatograph respectively, and determine.
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        [nature, taste and meridian tropism]   ; . .
        ; Dispel wind dampness and carbuncle. It is used for wind cold dampness arthralgia, limb contracture, arthralgia, carbuncle and ulceration.
        [usage and dosage]   1-3g. Suitable for external use.
        [note]   Forbidden for pregnant women.
        ; Store in a cool and dry place.


2、 Chemical composition of two tips
Rhizome containsoleanolic acid （oleanolicacid），Diosgenin（diosgenin）， Raddeanin R0Rhizoma CyperusA、Rhizoma CyperusB、Rhizoma CyperusC、Rhizoma CyperusD、Rhizoma CyperusE、Rhizoma CyperusF，Polygalactonin (paeoniflorin)the saponin of Anemone Polybotrys (L.)the main ingredient of paeoniflorin is paeoniflorin (L.)the major ingredient of paeoniflorin)the major ingredient of paeonifloriR8（raddeanoside）、 It is mainly composed of Anemone saponin R9, ranunculin, anemonin and Rhizoma Cyperi saponin h. Rhizoma Cyperi saponin A is also called anemodeanin a.

3、   Pharmacological action of Liangtoujian
1. Efficacy of two tips

Liangtoujian, a traditional Chinese medicine with pungent taste, hot nature and toxicity, can return to the spleen meridian. Dispelling wind dampness and detumescence are its most important medicinal effects. At ordinary times, it can be used for the treatment of human rheumatic pain, limb contracture and joint pain. The treatment is mainly taken in decoction, and the dosage is about one yuan. Sometimes it can also be used externally, and the dosage is appropriate for external use.

2. Two tips can cure joint pain

The effect of two tips on the treatment of human joint pain is obvious. If it can be combined with other traditional Chinese medicine, the analgesic effect will be better played. The specific usage in the treatment is two tips eight points, Fangfeng three coins, Achyranthes bidentata four coins and Clematis four coins, and finally add Songjie two coins and Caulis Spatholobi five coins. After these traditional Chinese medicine are prepared, soak them in water, and then fry them into soup before taking them.

3. Two tips can cure carbuncle, gangrene and sores

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