Chlorophytum chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis L.Var.chinensis
1、 Pharmacopoeia standard of Chlorophytum       This product is the dry aboveground part of Lysionotus pauciflorus maxim., a plant of Gesneriaceae. When the leaves are luxuriant in summer and autumn, they are harvested and cut to remove impurities and dried in the sun.
      [character] the stem of this product is cylindrical, 25-60cm long, 0.2-0.5cm in diameter; the surface is light brown or grayish brown, with longitudinal wrinkles, swollen nodes, and often adventitious roots; . Impeller is raw or opposite, with short handle; Most leaves fall off, and the petiole marks are obvious after falling off; The leaf blade is lanceolate to narrowly ovate, 1.5-6cm long and 0.5-1.5cm wide, with anti rolling edges, teeth on the upper part of the edge, gray green to gray brown on both sides. The breath is slight and the taste is bitter.
      [identification] (1) cross section of the stem of this product: the epidermis sometimes remains. The cork layer is composed of multiple rows of cork cells. ; The endothelial layer was evident. Phloem narrow. The cambium ring is not obvious. The xylem is connected into a ring by fibers and vessels. The medulla is larger.
      Transverse section of leaves: the upper epidermal cells are rectangular, covered with thin cuticle, and below them are 2-3 rows of large thin-walled cells, which are square, rectangular or round like; The cells of the lower epidermis were smaller and had stomata. ; Sponge tissue cells are round and loosely arranged. The vascular bundle of the main vein is tough on the outside, and a single stone cell is occasionally seen on the outside or near the lower epidermis.
      (2) Take 0.5g of this product powder, add 20ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate, add 1ml of methanol to dissolve the residue as the test solution. In addition, 0.2mg/1ml solution was prepared by adding methanol to the reference substance of clenbuterol as the reference substance solution. According to the test of thin-layer chromatography (general rule 0502), suck 10 μ l each of the above two solutions, dot them on the same silica gel G thin-layer plate, use chloroform methanol formic acid (20:1:0.5) as the developing agent, develop, take out, dry, spray 2% ferric chloride ethanol solution, and heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
      [check] moisture   .
      Total ash   Not more than 6.0%. (general rule 2302).
      [extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), using dilute ethanol as solvent, it shall not be less than 17.0%.
      [content determination] determine according to HPLC (general rule 0512).
      ; Octadecylsilane bonded silica gel was used as filler; Methanol water (68:32) was used as mobile phase; The detection wavelength was 334nm. The number of theoretical plates should not be less than 3000 according to the peak of phytolacanin.
      Preparation of reference solution take an appropriate amount of the reference substance of clenbuterol, weigh accurately, and add methanol to make a solution containing 25 μ g per 1ml.
      .
      The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      According to the calculation of dry product, the content of clenbuterol (c18h16o7) shall not be less than 0.10%.
      Decoction pieces
      [processing] remove impurities, wash, segment and dry.
      [nature, taste and meridian tropism] bitter, warm. Return to lung meridian.
      [functions and indications] it can dissipate phlegm, relieve cough, soften hardness and disperse nodules. It is used for cough with excessive phlegm and scrofula with sputum core.
      [usage and dosage] 9 ～ 15g. Apply appropriate amount externally, tamp or wash with boiling water.
      [storage] put it in a dry place.


2、 Chemical constituents of Chlorophytum
Whole grass containsClenbuterol, namely, nevada-nsin [1-3]

3、 Pharmacological effects of Chlorophytum
The bacteriostatic test of tubercle bacillus in vitro showed that lycopene (coumarin) had antibacterial effect at the concentration of 200 μ g/ml.