Shanxiangyuanye
1、 Pharmacopoeia standard of shanxiangyuanye       This product is the dried leaves of turpinia arguta seem. Harvest in summer and autumn when the leaves are luxuriant, remove impurities and dry in the sun.
      [properties] the product is oval or oblong, 7-22cm long and 2-6cm wide. The apex is tapered, the base is wedge-shaped, the edge is sparse serrated, near the base is entire, and the top of the serration has gland points. The upper surface is greenish brown and glossy; The lower surface is yellowish green, rough, the main vein is yellowish to light brown, protruding on the lower surface, and the lateral vein is feathery; The petiole is 0.5-lcm long. . It smells fragrant and tastes bitter.
      [identification] (1) cross section of this product: the upper epidermal cells are rectangular, covered by the stratum corneum, and scattered with round like oil cells. The lower epidermal cells were smaller; Apical sharp single-cell non glandular hairs can be seen. ; Sponge tissue is loose, and some cells contain calcium oxalate clusters. The main vein protrudes upward and downward, and the outer vascular bundle is tough and semicircular; Bundle sheath fiber bundles are arranged in discontinuous rings.
      (2) Take 2G of this product powder, add 50ml of water, decoct for 30 minutes, filter, concentrate the filtrate to about 20ml, shake with ether and extract twice, 20ml each time, discard the ether solution, saturate the water with n-butanol and extract three times, 20ml each time, combine the n-butanol solution, evaporate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test solution. In addition, 2G of the control medicinal material of Daphne odora rotundifolia was prepared into the control medicinal material solution by the same method. Then take the ligustrin reference substance and the vernicin reference substance, add methanol to make 1ml mixed solution containing 1mg each as the reference solution. . In the chromatogram of the test sample, fluorescent spots of the same color appear at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample.
      [inspection] the moisture content shall not exceed 13.0% (the fourth method of general rule 0832).
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      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol-0.5% phosphoric acid solution (43:57); .
      Preparation of reference solution take 10mg of ligustrin reference and 10mg of anacardin reference dried by phosphorus pentoxide under reduced pressure for 24 hours, weigh accurately, put them into 100ml volumetric flasks respectively, add 50% methanol to dissolve and dilute to the scale, and shake well; Accurately measure 25ml of ligustrin reference solution and 10ml of vernicin reference solution, put them into the same 50ml volumetric flask, add 50% methanol to dilute to the scale, shake well, and then obtain (each 1ml contains 50 μ g of ligustrin and 20 μ g of vernicin).
      Preparation of test solution take about 0.3g of powder (passing through No. 3 screen), weigh it accurately, place it in a corked conical flask, add 50ml of 50% methanol precisely, weigh it, sonicate (power 250W, frequency 25kHz) for 1 hour, cool it, weigh it again, make up the lost weight with 50% methanol, shake it well, filter it, and take the filtrate.
      The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      The content of ligustrin (c33h40o18) in this product shall not be less than 0.30% based on the dry product; The content of vernicin (c27h30o14) shall not be less than 0.10%.
      Decoction pieces
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      [nature, taste and meridian tropism] bitter, cold. Return to lung and liver channels.
      [functions and indications] it can clear away heat and detoxification, promote swallowing and detumescence, promote blood circulation and relieve pain. It is used for mastitis, sore throat, sores, swelling and poison, and falling pain.
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2、
The leaves contain 2 α, 3 β - dihydroxyurs-l2-en-28-oic acid, namely 2 α - hydroxyursolic acid, 2 α, 3 β, 19 α - trihydroxyurs-12-en-28-oic acid, namely 2 α, 19 α - dihydroxyursolic acid,α - geraniol(α-amyrin)，Ursolic acidUrsolic acid, 19 α - hydroxyursolic acid,Myristic acid(myristic acid)，Carotene(daucosterol)，nuezhenoside ， .

3、 Pharmacological effects of the leaves of Daphne odora
In vitro bacteriostatic test results showed that shanxiangyuan water decoction concentrate had strong bacteriostatic effect on Staphylococcus aureus, equivalent to 1U penicillin per 1ml, and had certain bacteriostatic effect on B hemolytic streptococcus, but not strong.