Callicarpa lobata
1、 Pharmacopoeia standard of Callicarpa lobata       This product is the dried leaves or leafy shoots of Callicarpa macrophyllavahl, a Verbenaceae plant. It is picked in summer and autumn and dried in the sun.
      [character] this product is often wrinkled, curled, and some are broken. ; The lower surface is light green or light brownish green, densely covered with gray white fluff, the main vein and lateral vein are protruding, the small vein extends to the tooth end, and gland spots can be seen on both sides. Apex acuminate, base cuneate or obtuse, margin serrate. The petiole is 0.8 ～ 2cm long and paper. It tastes pungent and bitter.
      [identification] (1) the powder of this product is grayish yellow to tan. There are two kinds of non glandular hairs: one is stellate hair, most of which are broken and lignified, and the intact one to several rounds, with 1-6 lateral cells in each round; The other non glandular hair has 1-3 cells with a diameter of 25-33 μ m and a thicker wall. Glandular scales have 8-11 cells in the head, oblate spherical shape, and extremely short stalks. The glandular hair has 2-4 cells in the head and 1-2 cells in the stalk. Calcium oxalate cluster crystals were fine and scattered in mesophyll cells.
      (2) Take LG of the product powder, add 30ml of ether, heat and reflux for 30 minutes, filter, evaporate the filtrate, add 2ml of methanol to dissolve the residue, and take the supernatant as the test solution. In addition, take ursolic acid reference substance and add methanol to make a solution containing 1mg per 1ml as the reference substance solution. . In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
      [inspection] the moisture content shall not exceed 15% (the second method of general rule 0832).
      The total ash content shall not exceed 11.0% (general rule 2302).
      [extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), using dilute ethanol as solvent, it shall not be less than 15.0%.
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-0.5% phosphoric acid solution (17:83); The detection wavelength was 332nm. The theoretical plate number should not be less than 3000 according to the calycosin peak.
      Preparation of reference solution take an appropriate amount of verbascoside reference substance, accurately weigh it, add 50% methanol to make a solution containing 50 μ g per 1ml.
      Preparation of test solution take about 0.25g of the powder (passing through No. 4 screen), accurately weigh it, place it in a corked conical flask, precisely add 50ml of 50% methanol, close the stopper, weigh it, place it overnight, heat and reflux for 1 hour, cool it, weigh it again, make up the weight loss with 50% methanol, shake it well, filter it, and take the filtrate.
      The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      According to the calculation of dry product, the content of verbascoside (c29h36ol5) shall not be less than 0.15%.
      Decoction pieces
      [processing] remove impurities, spray clean water, cut into sections, and dry.
      [nature, taste and meridian tropism] pungent, bitter and peaceful. Return to liver, lung and stomach channels.
      [functions and indications] dispel blood stasis and stop bleeding, reduce swelling and relieve pain. .
      [usage and dosage] 15 ～ 30g. Apply an appropriate amount externally, and apply it on the affected part after grinding.
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2、 Chemical constituents of Callicarpa lobata
cycasin β - sitosterol(β-sitosterol)，Luteolin(luteolin)，Apigenin(apigenin)，Luteolin-7-glucuronide(luteolin-7-glucuronide)，Apigenin-7-o-glucuronide(apigenin-7-o-glu-curonide), β - sitosterol - β - D-glucoside,Ursolic acid,Hawthorn acid(crategolic acid)， Docosanoic acid, tricosanoic acid, tetracosanoic acid, ethyl tricosanoate and 3,7,3 '- trimethoxy4', - 5-dihydroxyflavone. Two kinds of tetracyclic diterpenes are found in roots, aerial parts and leaves: calliterpenone and calliterpenone monoac etate

3、 Pharmacological effects of Callicarpa lobata
The leaves contain sitosterol, luteolin, apigenin, luteolin-7-o-glucuronide, apigenin-7-o-glucuronide, β - sitosterol - β - D-glucoside, ursolic acid, 2 α - hydroxyursolic acid, and crategolic acid, docosanoic acid, tricosanoic acid, Tetracosanoic acid, ethyl tricosanoate and 3,7,3 '- trimethoxy4' - and - 5-dihydroxyflavone. Two kinds of tetracyclic diterpenes are found in roots, aerial parts and leaves: calliterpenone and calliterpenone monoac etate