Eclipta
1、       This product is the dry aboveground part of ecliptaprostratal., a Compositae plant. The flowers are cut when they bloom and dried in the sun.
      [character] the product is covered with white fluff. The stem is cylindrical with longitudinal edges, with a diameter of 2-5mm; the surface is greenish brown or dark green. The leaves are opposite, nearly sessile, wrinkled, curled or broken, and the intact ones are long lanceolate after flattening, entire or with shallow teeth, dark green. . It tastes slightly salty.
      [identification] (1) take this product, soak it in water, rub its stems and leaves, showing dark green.
      (2) The leaf surface of this product: the non glandular hairs are mostly 3 cells, 260-700 μ m long, the base cells are slightly expanded, the middle cells are longer, the wall is thickened, there are obvious warty protrusions, the top cells are sharp and short, nearly triangular. The stomata are indeterminate, with 3-4 accessory guard cells.
      (3) Take 2G of this product powder, add 20ml of 70% methanol, sonicate for 45 minutes, filter, and take the filtrate as the test solution. . Then take an appropriate amount of ecliptin a reference substance and add methanol to make a solution containing 0.5mg per 1ml as the reference substance solution. According to the test of thin-layer chromatography (general rule 0502), suck 10 μ l of the test solution, 10 μ l of the reference drug solution and 5 ml of the reference solution, respectively, onto the same silica gel G thin-layer plate, use dichloromethane ethyl acetate methanol water (30:40:15:3) as the developing agent, develop, take out, air dry, spray vanillin sulfuric acid test solution, heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding positions of the chromatogram of the control medicinal material and the chromatogram of the control sample.
      [inspection] the moisture content shall not exceed 13.0% (the second method of general rule 0832).
      The total ash content shall not exceed 14.0% (general rule 2302).
      Acid insoluble ash content shall not exceed 3.0% (general rule 2302).
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Gradient elution was performed with methanol as mobile phase A and 0.5% acetic acid solution as mobile phase B according to the following table; .
      Time (min) mobile phase a (%)         Mobile phase B (%)
    0～10                 35→59           65→41
    10～20                59               41
      Preparation of reference solution take an appropriate amount of Wedelia chinensis lactone reference, weigh accurately, and add methanol to make a solution containing 10 μ g per 1ml.
      Preparation of test solution take about 1g of powder (passing through No. 3 screen), weigh accurately, place in a corked conical flask, add 50ml of 70% ethanol precisely, weigh, heat and reflux for 1 hour, cool, weigh again, make up the lost weight with 70% ethanol, shake well, filter, and take the filtrate.
      The determination method is to precisely suck 20 μ l of the reference solution and 20 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      Calculated as a dry product, the content of Wedelia chinensis lactone (c16h12o7) shall not be less than 0.040%.
      Decoction pieces
      [processing] remove impurities, wash slightly, cut into sections, and dry.
      This product has irregular sections. The stem is cylindrical, with green brown or dark green surface, longitudinal edges, white hairs, hollow section or white pith. The leaves are mostly wrinkled or broken, dark green, densely white hairy, and can be seen with full margin or light serration after flattening. Capitulum. It tastes slightly salty.
      [identification], [inspection], [content determination] are the same as those of medicinal materials.
      [nature, taste and meridian tropism] sweet, sour and cold. Return to kidney and liver channels.
      [functions and indications] nourish liver and kidney, cool blood and stop bleeding. It is used for liver and kidney yin deficiency, loose teeth, early white beard, dizzy tinnitus, waist and knee soreness, blood fever due to yin deficiency, hematemesis, hematuria, blood dysentery, metrorrhagia and bleeding due to trauma.
      [usage and dosage] 6 ～ 12g.
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2、 Chemical constituents of Eclipta
1. the whole herb contains 1.32% saponins, about 0.08% nicotine, tannin, vitamin A, ecliptine, and a variety of thiophene compounds such asA-terphenylmethanol, .
cycasin Wedelia chinensis lactone(Wedelolac-tone)、Demethylwedelia lactone. demethylwedelia lactone-7-glucoside. It contains about 0.08% nicotine, andA-terphenylmethanol(α－terthienylmethanol)、 α - formyl - α - terthienel; In addition, it also contains about 1.3% saponins, tannins, bitters and isoflavone glycosides.
cycasin Wedelia chinensis lactone(wedelolactone),Demethylwedelia lactoneDemethylwebelolactone and demethylwebelolactone glucoside.
Petroleum ether extract from aerial part containsStigmasterol, phytosterol a, andβ - aromatic resin(β－amyrin)； The ethanol extract also contains luteolin-7-o-glucoside, phytosterol a glucoside and a triterpenoid acid glucoside.

3、
1. hemostatic effect: cut the femoral artery of the dog in half, apply Mohan lotus leaf powder on the bleeding place, and slightly compress it, which has a good hemostatic effect. The aqueous extract also had significant hemostatic effect.
2. effect on cardiovascular system: the isolated guinea pig heart was used to observe that Herba Eclipta had the effect of increasing coronary flow and improving the changes of T wave of electrocardiogram. Some tests also showed that the injection of Eclipta philoxeroides under atmospheric hypoxia could significantly prolong the life of mice, and could also improve the survival rate of mice under the condition of decompression and hypoxia tolerance: the survival rate of the administration group was 76.5%, and that of the control group was 23.5%, P< 0.05; The difference between the two groups was significant. .
3. effects on the central nervous system: the sedative and analgesic effects of Eclipta philoxeroides on mice are very significant, and the effect of total flavonoids is not as good as that of total components.
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