Paris polyphylla
1、 Paris polyphylla pharmacopoeia standard       This product is the dried rhizome of Paris polyphylla Smith var. yunnanensis (franch.) hand. -mazz. or Paris polyphylla Smith var. chinensis (franch.) Hara, a Liliaceae plant. .
      ; It is densely covered with thick ring like laminar protrusions, with obvious nodules on one side, oval concave stem marks on the nodules, and sparse fibrous roots or warty fibrous root marks on the other side. Residues of apical scaly leaves and stems. It is solid, with flat section, white to light brown, powdery or horny. It is slightly bitter and numb.
      [identification] (1) the powder is white. There are many starch granules, which are round, long oval or kidney shaped, with a diameter of 3-18 μ M. Calcium oxalate needle crystals are in bundles or scattered, with a length of 80-250 μ M. The diameter of ladder shaped duct and reticulate duct is 10 ～ 25 μ M.
      (2) Take 0.5g of this product powder, add 10ml of ethanol, heat and reflux for 30 minutes, filter, and use the filtrate as the test solution. . According to the test of thin-layer chromatography (general rule 0502), suck 5 μ l each of the test solution and the reference medicinal material solution and 10 μ l of the reference solution under the item of [content determination], respectively dot on the same silica gel G thin-layer plate, use the lower layer solution of chloroform methanol water (15:5:1) as the developing agent, unfold, take out, dry, spray with 10% sulfuric acid ethanol solution, heat at 105 ℃ until the spots are clear, and inspect them under sunlight and ultraviolet light (365nm). In the chromatogram of the test sample, spots or fluorescent spots of the same color appear at the corresponding positions of the chromatogram of the control medicinal material and the chromatogram of the control sample.
      [inspection] the moisture content shall not exceed 12.0% (the second method of general rule 0832).
      The total ash content shall not exceed 6.0% (general rule 2302).
      Acid insoluble ash content shall not exceed 3.0% (general rule 2302).
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Use acetonitrile as mobile phase A and water as mobile phase B to perform gradient elution according to the following table; The detection wavelength was 203nm. The number of theoretical plates should not be less than 4000 based on the peak of Paris polyphylla saponin I.
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      Time (minutes)           ;                 Mobile phase B (%)
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    0～40             30→60              70→40
    40～50            60→30              40→70
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      Preparation of reference solution take appropriate amount of Paris polyphylla saponin I reference substance, Paris polyphylla saponin II reference substance, Paris polyphylla saponin VI reference substance and Paris polyphylla saponin VII reference substance, accurately weigh, add methanol to make a mixed solution containing 0.4mg per 1ml.
      Preparation of test solution take about 0.5g of the powder (passing through No. 3 screen), accurately weigh it, place it in a corked conical flask, precisely add 25ml of ethanol, weigh it, heat and reflux for 30 minutes, cool it, weigh it again, make up the lost weight with ethanol, shake it well, filter it, and take the filtrate.
      The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      The total amount of Paris polyphylla saponin I (c44h70o16), Paris polyphylla saponin II (c51h82o20)), Paris polyphylla saponin VI (c39h62o13) and Paris polyphylla saponin VII (c51h82o21) shall not be less than 0.60% according to the calculation of dried products.
      Decoction pieces
      .
      [nature, taste and meridian tropism] bitter, slightly cold; There is little poison. Return to liver meridian.
      [functions and indications] it can clear heat and detoxify, reduce swelling and pain, cool the liver and calm the shock. It is used for prickly sores, carbuncle, sore throat, snakebite, falling pain, convulsion.
      [usage and dosage] 3 ～ 9g. Apply appropriate amount externally, and adjust at the end of research.
      [storage] store in a cool and dry place to prevent moths.


2、 Chemical composition of Paris polyphylla
It contains a variety of steroidal saponins and isDiosgenin(diosgenin) and the di -, tri -, and tetra glucosides of pennogenin, which also containβ - ecdysone、CaroteneEt al.


3、 Pharmacological effects of Paris polyphylla
Treatment of chronic tracheitis: peel, mash, grind and press paris rhizome. 3G each time, twice a day, after meals. Ten days is a course of treatment, a total of three courses of treatment, and the drug was stopped for 3 days between each course of treatment. More than 250 cases were treated. ; 122 cases were treated in the second course of treatment, and the effective rate was 96.7%; 92 cases were treated in the whole course, and the effective rate was 97.3%.