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Magnolia officinalis
Houpohua
MAGNOLIAE OFFICINALIS FLOS
[properties] this product is long conical, 4-7cm long, 1.5-2.5cm in base diameter. It is reddish brown to tan. . The stamens are numerous, the anthers are strip-shaped, yellowish brown, and the filaments are wide and short. Carpels are numerous, separated, spirally arranged on the cone shaped receptacle. The pedicel is 0.5 ～ 2cm long, densely covered with grayish yellow villi, and occasionally hairless. It is brittle and easy to break. It smells fragrant and tastes light.
[identification] (1) the powder of this product is reddish brown. The epidermal cells of perianth are polygonal or oval, with dense verrucous protrusions on the surface, and some have fine strip texture. The stone cells are numerous, irregular and branched, with a wall thickness of 7-13 μ m, obvious pore grooves, and large cell cavities. The oil cells are round and oval in shape, with a diameter of 37 ～ 85 μ m, a slightly thick wall and yellow brown substance. . Non glandular hairs are 1-3 cells, 820-230 μ m long, extremely thick wall, and some have a spiral keratinocyte texture on the surface. The apex of single-cell ones is long and pointed, and the base is slightly expanded. The basal cells of multicellular ones are shorter or significantly expanded, and the wall is thin.
(2) . In addition, take magnolol reference substance and magnolol reference substance, add methanol to make a mixed solution containing 1mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 10 μ l of the test solution and 5 μ l of the control solution, respectively dot on the same silica gel G thin-layer plate, use cyclohexane dichloromethane ethyl acetate concentrated ammonia test solution (5:2:4:0.5) as the developing agent, develop, take out, air dry, spray 1% vanillin sulfuric acid solution, heat at 100 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
[inspection] the moisture content shall not exceed 10.0% (the third method of general rule 0832).
The total ash content shall not exceed 7.0% (general rule 2302).
[content determination] determine according to HPLC (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol acetonitrile water (50:20:30); The detection wavelength was 294nm. The number of theoretical plates should not be less than 1500 according to the magnolol peak.
Preparation of reference solution take a proper amount of magnolol reference substance and honokiol reference substance, accurately weigh them, add methanol to make a solution containing 60 μ g of magnolol and 40 μ g of magnolol per 1ml, respectively.
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The determination method is to precisely suck 10 μ l of each of the above two reference solutions and the test solution, inject them into the liquid chromatograph, and determine.
The total content of magnolol (c18h18o2) and honokiol (c18h18o2) in this product shall not be less than 0.20%.
. Return to spleen and stomach meridian.
[functions and indications] aromatics can remove dampness and regulate qi. .
[usage and dosage] 3 ～ 9g.
[storage] put it in a dry place to prevent mold and moth.
2、 Chemical constituents of Magnolia officinalis
Flower bud containsMagnolol（magnolol），Honokiol(honokiol) andcamphor（camphor）[1]。
3、 Pharmacological effects of Magnolia officinalis
Water soluble tincture of Magnolia officinalis by intravenous or intramuscular injection in anesthetized rabbits and cats can reduce blood pressure and accelerate heart rate.