Honeysuckle vine
1、 Pharmacopoeia standard of Lonicera japonica Thunb       This product is the dried stem and branch of Lonicera japora'ca thunb., a plant of the family Lonicerae. Harvest and dry in autumn and winter.
      [character]   This product is long cylindrical, multi branched, often wound into bundles, with a diameter of 1.5 ~ 6mm. The surface is brownish red to dark brown, and some are grayish green, smooth or downy; . There are many nodes on the branches, and the internode is 6 ~ 9cm long, with residual leaves and leaf marks. It is brittle and easy to break. Its cross section is yellowish white and hollow. The air is weak, the old branches are slightly bitter, and the tender branches are light.
      [identification]   (1) The powder of this product is light brown yellow to yellowish brown. . The epidermal cells are brownish yellow to brownish red, with a polygonal appearance on the surface, and often have traces of non glandular hairs after shedding, which are stone cell like. The parenchyma cells contain calcium oxalate clusters, which are often arranged in rows, and some are scattered individually, with blunt edges and corners, with a diameter of 5-15 μ M.
      (2) Take 1g of this product powder, add 10ml of 50% methanol, sonicate for 30 minutes, filter, and take the filtrate as the test solution. Another 1g of Caulis Lonicerae Japonicae reference material was prepared into the reference material solution by the same method. . According to the test of thin-layer chromatography (general rule 0502), suck 10 μ l of the test solution, 10 μ l of the control medicinal material solution and 5 μ l of the control solution, respectively, and dot them on the same silica gel G thin-layer plate. Use the lower layer solution of chloroform methanol water (65:35:10) placed below 10 ℃ as the developing agent, develop, take out, air dry, spray 10% sulfuric acid ethanol solution, and heat at 105 ℃ until the spots are clear. In the chromatogram of the test sample, spots with the same color appear at the corresponding positions of the chromatogram of the control medicinal material and the chromatogram of the control sample.
      [check]   Moisture   .
      Total ash   Not more than 4.0% (general rule 2302).
      [extract]   According to the hot leaching method under the determination of alcohol soluble extract (general rule 2201), 50% ethanol shall be used as solvent, which shall not be less than 14.0%.
      [content determination]   Chlorogenic acid   Determine according to high-performance liquid chromatography (general rule 0512).
      ; Octadecylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-0.4% phosphoric acid solution (10:90); The detection wavelength is 327nm. The number of theoretical plates should not be less than 1000 according to the chlorogenic acid peak.
      Preparation of reference solution   Take an appropriate amount of chlorogenic acid reference substance, weigh accurately, add 50% methanol to make a solution containing 40 μ l per 1ml.
      Preparation of test solution   Take about 1g of this product powder (passing through No. 3 screen), weigh it precisely, place it in a corked conical flask, add 25ml of 50% methanol precisely, weigh it, sonicate (power 250W, frequency 30kHz) for 30 minutes, cool it, weigh it again, make up the lost weight with 50% methanol, shake it well, filter it, and take the filtrate.
      ; Precisely suck 10 μ l of the reference solution and 5 ～ 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      According to the calculation of dry product, the content of chlorogenic acid (c16h18o9) shall not be less than 0.10%.
      Loganin   Determine according to high-performance liquid chromatography (general rule 0512).
      ; Phenylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-0.4% phosphoric acid solution (12:88); The detection wavelength was 236nm. The number of theoretical plates should not be less than 3000 according to loganin peak.
      Preparation of reference solution   Take an appropriate amount of loganin reference substance, weigh accurately, add 50% methanol to make a solution containing 40 μ g per 1ml
      Preparation of test solution   Take about 1g of this product powder (passing through No. 3 screen), weigh it precisely, place it in a corked conical flask, add 25ml of 50% methanol precisely, weigh it, sonicate (power 500W, frequency 40KHz) for 30 minutes, cool it, weigh it again, make up the lost weight with 50% methanol, shake it well, filter it, and take the filtrate.
      Assay   Precisely suck 10 μ l of the reference solution and 2 ～ 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
      According to the calculation of dry product, the content of loganin (c17h26o10) shall not be less than 0.10%.
      Decoction pieces
      [processing]   Remove impurities, wash, moisten, cut into sections, and dry.
      This product has irregular sections. The surface is brownish red (twigs), some are grayish green, smooth or downy; The skin is easy to fall off. ; Occasionally, there are residual leaves, dark green, slightly hairy. The air is weak, the old branches are slightly bitter, and the tender branches are light.
      [content determination]   The content of chlorogenic acid (c16h18o9) in the same medicinal material shall not be less than 0.070%.
      [identification], [inspection], [extract], [content determination] (brucine) is the same as that of medicinal materials.
      [nature, taste and meridian tropism]   Gan, Han. Return to lung and stomach channels.
      ; It can clear away heat, detoxify, dredge wind and unblock collaterals. It is used for febrile diseases, heat toxin and blood dysentery, carbuncle and sore, rheumatism and heat arthralgia, joint redness, swelling and heat pain.
      [usage and dosage]   9～30g。
      [storage]   Place in a dry place.


2、 Chemical constituents of Lonicera japonica Thunb
Rattan containschlorogenic acid（chlorogenic acid），Isochlorogenic acid A（isochlorogenicacid）、Isochlorogenic acid B、Isochlorogenic acid C. the aboveground part containsBrucine（loganin）， Secologanindimethylacetal, vogeloside, EPI vogeloside [2], hederagenin-3-o - α - l-arabinopyranoside, hederagenin-3-o - β - D-glucopyranosyl (1 → 2) - α - l-arabinopyranoside,d-glucopyranosyl (1 → 2) - α - l-arabinopyranoside], Hederagenin-3-o - α - l-arabinopyranosyl-28-o - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside [hederagenin-3-o - α - l-arabinopyranosyl-28-o - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside], , Hederagenin-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-rhamnopyranosyl-28-o - β - D-glucopyranosyl [hederagenin-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o - β - d-glucose-ranoside,] hederagenin-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o - β - d- glucopyranosyl (1 → 6) - β - d-glucopyranoside [hederagenin-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-arabinopyran-nosyl-28-o - β - d-glucopyranan Osyl (1 → 6) - β - d-glucopyranoside], Ivy saponin-3-o - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside], Ivy saponin-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o-3-acetyl - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside [hederagenin-3-o - α - l-rhamnopyranosyl (1 → 6))1 → 2) - α - l-arabinopyran-nosyl-28-o-6-acetyl - β - d-glucogranosyl (1 → 6) - β - o-glucopyrano-side], Oleanolic acid-3-o - β - D-glucopyranosyl (1 → 2) - α - l-arabinopyranoside [oleanolicacid-3-o - β - D-glucopyranosyl (1 → 2) - α - l-arabinopyranoside [oleanolicacid] - 3-O - β - D-glucopyranosyl (1 → 2) - α - l-arabinopyranoside-28-o - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside [oleanolicacid-3-o - α - l-ara-binopyranosyl -28-o- β - D-glucopyranosyl (1 → 6) - β - d-glucopyran-noside], Oleanolic acid-3-o - β - D-glucopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside [oleanolic acid-3-o - β - D-glucopyranosyl (1 → 2) - α - l-arabinopyran-nosyl-28-o - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside], Oleanolic acid-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o - β - D-glucopyranosyl (1 → 6) - β - d-glucopyranoside [oleanolicacid-3-o - α - l-rhamnopyranosyl () 1 → 2]- α - l-arabinopyranosyl-28-o - β - d-glu-copyranosyl (1 → 6) - β - d-glucopyranoside][3], Ivy saponin-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o-3-acetyl - β - d-xylopyranosyl - α - l-arabinopyranosyl (1- α - l-arabinopyranosyl 6) - β - d-glucopyranoside [hederagenin-3-o - α - l-rhamnopyranosyl (1- α - l-arabinopyranosyl (1 → 6) - β - d-glucopyranoside [hederagenin-3-o - α -l-rhamnopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o-3-acetyl - β - d-xylopyranosyl (1 → 6) - β - d-glucopyranoside] Hederagenin-3-o - α - l-rhamnopyranosyl (1 → 2) - α - l-arabinopyranosyl-28-o - β - d-xylopyranosyl (1 → 6) - β - d-glucopyranoside [gederangenin-3-o - β - d-xylopyranosyl (1 → 6) - β - d-glucopyranoside [hederagenin-3-o - α - l-rhamnopyranosyl (1 → 6) - β - d-glucopyranoside][4], also contains iron, barium, manganese and zinc, titanium, strontium, copper and other trace elements [5].

Young branches containDeoxybrucine glycoside De De De De De oxidation of brucine in the presence of oxygen free radical scavenging agent, brucine glucoside de oxidation of brucine glucoside, brucine glucoside de oxidation of brucine glucoside, brucine（secoxyloganin）[6].。

cycasin Luteolin（luteolin）， Loniceraflavone, 3-methoxy-5,7,4-trihydroxyflavone, luteolin-7-rhamnoglucoside, luteolin-7-o-digalactoside, loniceraflavone-6-rhamnoglucoside, isochlorogenic acid andcaffeic acid（caffeicacid）[7]，Vanillic acid(vanillicacid) and camptothecin [8].

The aerial part of Lonicera japonica contains l-4-hydroxy-2,6-di - (4-Hydroxy-3-Methoxy) phenyl-3,7-dioxobicyclo [3,3,0] octane [4-hydroxy-2,6-di- (4-hy-hydroxy-3-methoxy) phenyl-3,7-dioxobicyclo[3,3,0]octane], n-10-nonaconanol,Scopoletin Pavilion（scopoletin），Butyric acid（syringicacid），β - sitosterol(β -sitosterol) and β -sitosterolglucoside [9]

3、 Pharmacological effects of Lonicera japonica Thunb
1. antibacterial and anti-inflammatory effects: luteolin contained in this product can inhibit the growth of Staphylococcus and Bacillus subtilis at a concentration of 1:350000. It also has inhibitory effect on catarrhal, white rosary, typhoid, dysentery, deformation and other bacteria. The 50% Decoction of the aerial part of Lonicera japonica has inhibitory effect on Salmonella typhi, Shigella flexneri, Staphylococcus aureus and Pseudomonas aeruginosa by the method of digging trenches on a flat plate; . It also has strong anti infection effect in vivo. It has a strong inhibitory effect on h.su-is virus. At a dose of 20mg / kg × 7d, it can significantly inhibit the inflammatory process caused by implantation of wool balls in rats.

2. cardiovascular effects: luteolin at a concentration of 5 × 10 can reduce the diastolic amplitude of isolated frog heart, slightly reduce the systolic amplitude, slow down the heart rate, and reduce the output. At the concentration of 2 × 10, it can increase the systolic and diastolic amplitude of isolated guinea pig heart, and the heart rate becomes faster, but it has no direct effect on coronary artery and blood volume. For the cardiopulmonary device in dogs, 5-10mg luteolin can increase the arterial pressure and reduce the venous pressure, 10mg can reduce the lower limb blood flow in rats (33%), and 5-15mg can increase the blood pressure in cats and dogs by 12-30%. The above effects cannot be blocked by α - adrenergic blockers. Subcutaneous injection of 0.5g / kg in rats has the effect of enhancing capillaries. .

3. antispasmodic effect: luteolin has antispasmodic effect on isolated rabbit small intestine, guinea pig tracheal smooth muscle and ileum.

4. other effects: luteolin has good expectorant effect by phenol red method in mice and capillary method in rats. Luteolin can inhibit the growth of NK / ly ascites cancer cells in vitro.