Dragon's blood
1、 Pharmacopoeia standard for blood exhaustionThis product is made from processed resin extracted from the fruit of Daemonorops Draco Bl., a plant in the palm family.
【 Appearance 】 This product is slightly round, square or brick shaped, with a dark red surface and a glossy finish, accompanied by red powder formed by friction. Hard and brittle in texture, with a red broken surface and a brick red powder. The air is faint and the taste is light. Insoluble in water, softens in hot water.
【 Identification 】 (1) Take the powder of this product, place it on white paper, and bake it with fire over the paper to melt it, but there is no spreading oil stain, and it appears bright red under light. Burning with fire produces pungent smoke.
(2) Take 0.1g of the powder of this product, add 10ml of ether, seal, shake for 10 minutes, filter, and take the filtrate as the test solution. Take 0.1g of the reference medicinal material for blood exhaustion and prepare a reference medicinal material solution using the same method. According to the thin layer chromatography method (General Rule 0502), take 10-20 µ l of the test sample solution, reference herbal medicine solution, and reference solution of the [content determination] item, and place them on the same silica gel G thin layer plate. Use chloroform methanol (19:1) as the developing agent, unfold, remove, and air dry. In the chromatogram of the test sample, the same orange spots appear at the corresponding positions of the chromatogram of the control medicinal material and the chromatogram of the control sample.
(3) Take the powder of this product 5g， Add 10ml of ethanol, seal tightly, shake for 10 minutes, filter, add 5ml of dilute hydrochloric acid to the filtrate, mix well, precipitate a brownish yellow precipitate, and gradually solidify into a brownish black resin like substance after storage. Take the resin like substance, wash it thoroughly with 10ml of dilute hydrochloric acid, discard the washing solution, add 10ml of 20% potassium hydroxide solution, grind, add 5ml of chloroform and shake for extraction. The chloroform layer appears red, and the chloroform solution is taken as the test sample solution. Take another 0.5g of the reference medicinal material of blood exhaustion and prepare a reference medicinal material solution using the same method. According to the thin layer chromatography method (General Rule 0502), take 10-20 µ l of each of the above two solutions and dot them onto the same silica gel G thin layer plate. Use chloroform methanol (19:1) as the developing agent, develop, remove, and air dry. In the chromatogram of the test sample, the same orange spot appears at the corresponding position as the chromatogram of the control medicinal material.
[Inspection] The total ash content shall not exceed 6.0% (General Rule 2302).
Take 0.1g of rosin powder and place it in a stoppered test tube. Add 10ml of petroleum ether (60-90 ℃), shake for several minutes, filter, take 5ml of the filtrate, place it in another test tube, add 5ml of newly prepared 0.5% copper acetate solution, shake, let it stand and separate layers. The petroleum ether layer should not appear green.
Alcohol insoluble substance: Take about 2g of the powder of this product, accurately weigh it, place it in a filter paper cylinder of known weight, place it in a Soxhlet extractor, add 200-400ml of ethanol, reflux and extract until the extract is colorless, take out the filter paper cylinder, evaporate the ethanol, dry at 105 ℃ for 4 hours, accurately weigh and calculate, it should not exceed 25.0%.
[Content determination] Determine according to the high-performance liquid chromatography method (General Rule 0512).
Chromatographic conditions and system suitability test using octadecylsilane bonded silica gel as filler; Using acetonitrile 0.05mol/L sodium dihydrogen phosphate solution (50:50) as the mobile phase; The detection wavelength is 440nm and the column temperature is 40 ℃. The number of theoretical plates should not be less than 4000, calculated based on the peak of blood stasis.
Preparation of reference solution: Take 9mg of the reference substance of Draconis perchlorate, accurately weigh it, and place it in a 50ml brown volumetric flask. Add 3% phosphoric acid methanol solution to dissolve it and dilute it to the mark. Shake well. Accurately measure 1ml and place it in a 5ml brown volumetric flask. Add methanol to the mark and shake well to obtain (26 µ g of Draconis per 1ml) (Draconis weight=Draconis perchlorate weight/1.377).
Preparation of test solution: Take an appropriate amount of this product, grind it finely, take 0.05-0.15g, weigh it accurately, place it in a stoppered test tube, add 10ml of 3% phosphoric acid methanol solution accurately, seal the stoppered tube, shake for 3 minutes, filter, accurately measure 1ml of the filtrate, place it in a 5ml brown volumetric flask, add methanol to the mark, shake well, and it is ready.
The determination method involves precisely taking 10 µ l of the reference solution and 10 µ l of the test solution, injecting them into the liquid chromatograph, and measuring the results.
This product contains not less than 1.0% of sanguinarine (C17H14O3).
decoction pieces
[Processing] Remove impurities, grind into small pieces or grind into fine powder.
Sweet, salty, and flat. Returning to the heart and liver meridian.
【 Functions and Indications 】 Promote blood circulation and relieve pain, remove blood stasis and stop bleeding, and promote muscle healing and wound healing. Used for injuries caused by falls, chest and abdominal bruises, bleeding from external trauma, and ulcers that do not heal.
【 Usage and Dosage 】 Grind to powder, 1-2g, or take as a pill. For external use, sprinkle powder or apply ointment for medicinal purposes.
【 Storage 】 Store in a cool and dry place.


2、 Chemical composition of blood exhaustion
The branch lipids secreted by the scales on the surface of Qilin fruit contain Dracorubin,Hematospermin(dracorhodin)， Nordracorubin, Nordracorhodin, (2S) -5-methoxy-6-methylflavan-7-ol [(2S) -5-methoxyflavan-7-ol], (2S) -5-methoxyflavan-7-ol [(2S) -5-methoxyflavan-7-ol], 2,4-dihydroxy-5-methyl-6-methoxychalcone, Dracofavan A, Dracoxepine, and other compoundsHaipine acid(pimaricacid)， Isopimaric acid,Abietic acid(abieticacid)，dehydroabietic acid (dehydroabieticacid)， Sandaracopimaric acid.

3、 Pharmacological effects of blood exhaustion
1. Antifungal effect
2. Hemostatic effect