Woody Butterfly

1、 Pharmacopoeia standard of woodbutterfly       This product is the dried and mature seed of oroxylum indicum (L.) vent., a plant of Bignoniaceae. Harvest the mature fruits in autumn and winter, expose them to the sun until they crack, remove the seeds, and dry them in the sun.
      [properties] this product is butterfly shaped thin section, which extends into wide and thin wings on three sides except the base, with a length of 5-8cm and a width of 3.5-4.5cm. The surface is light yellow white, the wings are translucent, with silk like luster, radial texture on it, and the edges are often broken. The body is light, and the seed coat is stripped, and a thin film-like endosperm can be seen tightly wrapped outside the cotyledons. Cotyledons 2, butterfly shaped, yellowish green or yellow, 1-1.5cm long in diameter. It has a slight air and bitter taste.
      [identification] (1) the powder of this product is yellow or yellowish green. The seed wing cells are long and fibrous, and the wall is wavy and thickened, with a diameter of 20-40 μ M. Endosperm cells are polygonal, and the wall is Rosary thickened.
      (2) Take 0.1g of this product powder, add 25ml of methanol, sonicate for 30 minutes, filter, and take the filtrate as the test solution. In addition, 0.1mg and 1mg solutions per 1ml of xylobioside B control and baicalin control were prepared by adding methanol as the control solution. Test according to thin-layer chromatography (general rule 0502), suck 5 μ l of each of the above three solutions, dot them on the same polyamide film respectively, use acetic acid as the developing agent, develop, take out, dry, and view under the UV light (365nm). In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample; .
      [check] moisture   Not more than 6.0% (the second method of general rule 0832).
      [extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), 70% ethanol shall be used as solvent, which shall not be less than 20.0%.
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test   Octadecylsilane bonded silica gel was used as filler; ; The detection wavelength was 276nm. The number of theoretical plates should not be less than 2000 according to the peak of xylobioside B.
      Preparation of reference solution   Take an appropriate amount of the reference substance of xylobioside B, weigh it accurately, add 50% methanol to make a solution containing 0.10mg per 1ml.
      Preparation of test solution   Take about 0.1g of this product powder (passing through No. 2 screen), weigh it accurately, place it in a corked conical flask, add 50ml of methanol precisely, weigh it, heat and reflux for 1 hour, cool it, weigh it again, make up the lost weight with methanol, shake it well, filter it, and take the filtrate.
      Assay   Precisely suck 10 μ l of the reference solution and 10 μ l of the test solution respectively, inject them into the liquid chromatograph, and determine.
      According to the calculation of dry product, the content of xylobioside B (c27h30o15) shall not be less than 2.0%.
      [nature, taste and meridian tropism] bitter, sweet and cool. .
      [functions and indications] clearing lung and throat, soothing liver and stomach. For lung heat cough, throat obstruction, hoarseness, liver and stomach qi pain.
      [usage and dosage] 1 ～ 3G.
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2、 Chemical constituents of woody Butterfly
Seeds contain 20% fatty oil, of which oleic acid accounts for 80.4%[1]. It also contains benzoicacid,Chrysin（chrysin），Oroxin a、XylobiosideB[2] , baicalein, tetuin [3], 5-hydroxy-6,7-dimethoxyflavone,Lignocellulin（oroxylin）A， 5,6-dihydroxy-7-methoxyflavone,Ambrosin（hispidulin），Apigenin（apigenin）， Scutel larein, chrysin-7-o - β - d-glucopyra-noside, chrysin-7-o - β - gen tiobioside,Baicalin（baicalin），Baicalin（scutellarin）[4]，Woody Butterfly.

The leaves contain baicalein, alpine baicaleinBaicalin[6]。

3、 Pharmacological action of woody Butterfly
Seeds and stem bark contain baicalin, which has anti-inflammatory, anti allergic, diuretic, cholagogic and cholesterol lowering effects. Chrysin contained in seeds and stem bark has cytotoxic activity against human nasopharyngeal carcinoma (KB) cells, and its half effective amount is 13mg/ml.