1、 Pharmacopoeia standard of chuanmuxiang
This product is the dried root of the Compositae plant vladimiria souliei (franch.) Ling or vladimiria souliei (franch.) Ling var.cinerea Ling. Excavate in autumn, remove fibrous roots, sediment and colloids on the root heads, and dry them.
; Occasionally, there are black and sticky gums at the root, which is often called "oil head". It is light, hard and brittle, and easy to break. The section is yellowish white or yellow, with dark yellow sparse oil spots and cracks. The wood is broad and has radial texture; Some centers are withered. It smells slightly fragrant and tastes bitter. It sticks to the teeth when chewing.
[identification] (1) cross section of this product: the cork layer is a series of brown cells. ; The sieve tube groups and fiber bundles, as well as the xylem duct groups and fiber bundles are arranged in an interactive radial manner, in a neat radial shape. The cambium was wavy curved, with yellow fiber bundles, lignified, and accompanied by stone cells. The marrow is intact or ruptured. Oil chambers are scattered in the rays or medullary parenchyma. Inulin can be seen in parenchyma cells.
(2) Take 2G of this product powder, add 20ml of diethyl ether, sonicate for 20 minutes, filter, volatilize the filtrate, add 1ml of methanol to dissolve the residue as the test solution. Another 2G of chuanmuxiang reference medicinal material was prepared into the reference medicinal material solution by the same method. . In the chromatogram of the test sample, spots of the same color appear on the corresponding position of the chromatogram of the control medicinal material.
[inspection] the moisture content shall not exceed 12.0% (the fourth method of general rule 0832).
The total ash content shall not exceed 4.0% (general rule 2302).
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Methanol water (65:35) was used as mobile phase; The detection wavelength was 225nm. The number of theoretical plates should not be less than 6000 according to the peak of aucklan lactone. Preparation of reference solution take an appropriate amount of aucklandine lactone reference and dehydrocaucklandine reference, weigh accurately, add methanol to make a mixed solution containing 0.1mg per 1ml.
Preparation of test solution take about 0.3g of powder (passing through No. 4 screen), weigh accurately, place in a corked conical flask, add 50ml of methanol precisely, close the stopper, weigh, place overnight, sonicate (power 250W, frequency 50KHz) for 30 minutes, take out, cool, weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
This product is calculated as dry product and contains aucklandin lactone (c15H20O2）15H18O2）The total amount shall not be less than 3.2%.
Decoction pieces
[processing] chuanmuxiang removes the black "oil head" and impurities on the head of the root. It is washed, moistened, cut into thick pieces, and dried in the air or at low temperature. This product is in quasi circular section, with a diameter of 1.5 ~ 3cm. The outer skin is yellowish brown to tan. . It smells slightly fragrant and tastes bitter. It sticks to the teeth when chewing.
[identification] (except for the cross-sectional surface) [inspection] (moisture) [content determination] is the same as that of medicinal materials.
Simmer chuanmuxiang to remove the chuanmuxiang tablets. In the iron wire plaque, use a layer of straw paper and a layer of chuanmuxiang tablets, tile them several layers at intervals, place them next to the fire or in the drying room, bake and simmer until the volatile oil contained in chuanmuxiang seeps onto the paper, take them out, and let them cool. This product is shaped like chuanmuxiangpian, slightly fragrant, bitter, and sticky to the teeth.
[identification] (except for the cross-sectional surface) [inspection] (moisture) [content determination] is the same as that of medicinal materials.
[nature, taste and meridian tropism] pungent, bitter and warm. .
[function and indications] promoting qi and relieving pain. It is used for chest and flank pain, abdominal distension and pain, bowel sounds and diarrhea, and tenesmus.
.
[storage] store in a cool and dry place.
; Occasionally, there are black and sticky gums at the root, which is often called "oil head". It is light, hard and brittle, and easy to break. The section is yellowish white or yellow, with dark yellow sparse oil spots and cracks. The wood is broad and has radial texture; Some centers are withered. It smells slightly fragrant and tastes bitter. It sticks to the teeth when chewing.
[identification] (1) cross section of this product: the cork layer is a series of brown cells. ; The sieve tube groups and fiber bundles, as well as the xylem duct groups and fiber bundles are arranged in an interactive radial manner, in a neat radial shape. The cambium was wavy curved, with yellow fiber bundles, lignified, and accompanied by stone cells. The marrow is intact or ruptured. Oil chambers are scattered in the rays or medullary parenchyma. Inulin can be seen in parenchyma cells.
(2) Take 2G of this product powder, add 20ml of diethyl ether, sonicate for 20 minutes, filter, volatilize the filtrate, add 1ml of methanol to dissolve the residue as the test solution. Another 2G of chuanmuxiang reference medicinal material was prepared into the reference medicinal material solution by the same method. . In the chromatogram of the test sample, spots of the same color appear on the corresponding position of the chromatogram of the control medicinal material.
[inspection] the moisture content shall not exceed 12.0% (the fourth method of general rule 0832).
The total ash content shall not exceed 4.0% (general rule 2302).
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Methanol water (65:35) was used as mobile phase; The detection wavelength was 225nm. The number of theoretical plates should not be less than 6000 according to the peak of aucklan lactone. Preparation of reference solution take an appropriate amount of aucklandine lactone reference and dehydrocaucklandine reference, weigh accurately, add methanol to make a mixed solution containing 0.1mg per 1ml.
Preparation of test solution take about 0.3g of powder (passing through No. 4 screen), weigh accurately, place in a corked conical flask, add 50ml of methanol precisely, close the stopper, weigh, place overnight, sonicate (power 250W, frequency 50KHz) for 30 minutes, take out, cool, weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
This product is calculated as dry product and contains aucklandin lactone (c15H20O2）15H18O2）The total amount shall not be less than 3.2%.
Decoction pieces
[processing] chuanmuxiang removes the black "oil head" and impurities on the head of the root. It is washed, moistened, cut into thick pieces, and dried in the air or at low temperature. This product is in quasi circular section, with a diameter of 1.5 ~ 3cm. The outer skin is yellowish brown to tan. . It smells slightly fragrant and tastes bitter. It sticks to the teeth when chewing.
[identification] (except for the cross-sectional surface) [inspection] (moisture) [content determination] is the same as that of medicinal materials.
Simmer chuanmuxiang to remove the chuanmuxiang tablets. In the iron wire plaque, use a layer of straw paper and a layer of chuanmuxiang tablets, tile them several layers at intervals, place them next to the fire or in the drying room, bake and simmer until the volatile oil contained in chuanmuxiang seeps onto the paper, take them out, and let them cool. This product is shaped like chuanmuxiangpian, slightly fragrant, bitter, and sticky to the teeth.
[identification] (except for the cross-sectional surface) [inspection] (moisture) [content determination] is the same as that of medicinal materials.
[nature, taste and meridian tropism] pungent, bitter and warm. .
[function and indications] promoting qi and relieving pain. It is used for chest and flank pain, abdominal distension and pain, bowel sounds and diarrhea, and tenesmus.
.
[storage] store in a cool and dry place.
2、 Chemical constituents of chuanmuxiang
Volatile oil was isolated from the root of chuanmuxianggen with a yield of 0.8%. 26 components were identified by gc/ms, and the main components of the volatile oil wereDehydrocosthol(dehydrocostuslactone)。 , 6 α -olide], 3 β - acetoxy-11 β h-guaiac-4 (15), 10 (14) - dien-12, 6 α - lactone [3 β -acetoxy-11 β h-guaia-4 (15), 10 (14) - diene-12, 6 α -olide], 10 α, 14 epoxy ll β h-guaiac-4 (15) ene-12, 6 α - lactone [10 α, 14-epoxy-11 β h-guaia-4 (15) -ene-12, 6 α, olide], 3 β - hydroxy-10 α, 14-epoxy-4 β, 11 β h-guaiac-12, 6 α - lactone (3 β -hydroxy-10 α, 14-epoxy-4 β, 11 β h-guaiau-12, 6 α -olide), 10 β, 14-dihydroxy-11 β h-guaiacol-4 (15) - ene-12, 6 α - lactone [10 β, 14 dihydroxy ll β h-guaia-4 (15) -ene-12, 6 α -olide], 3 β, 11 β - dihydroxy guaiacol-4 (15), 10 (14) - diene-12, 6 α - lactone [3 β, 11 β -dihdroxyguaia-4 (15), 10 (14) -diene-12, 6 α -otide], 11 α h-guaiacol-4 (15), 10 (14) - diene-12, 6 α- lactone [ll α h-gu α ia-4 (15), 10 (14) -diene-12, 6 α -olide], 3 β - hydroxy-11 α h-guaiac-4 (15), 10 (14) - diene-12, 6 α - lactone [3 β -hydroxy-11 α h-guaia-4 (15), 10 (14) -diene-12, 6 α -olidj, 10 β, 14-dihydroxy-ll α h-guaiac-4 (15) - en-12, 6 α - lactone [10 β, 14-dihydroxy-ll α h-guaia-4 (15) -ene-12, 6 α -olide], 1 β, 2 α - hydroxy-11 β h-eucalyptus-4 (15) en-12, 6 α - lactone [1 β, 2 α - dihydroxy-11 β h-eu β - desm-4 (15) -ene-12, 6 α -olide], 1 β, 4 α - dihydroxy-11 β h-cineole-12, 6-lactone (1 β, 4 α -dihydroxy-11 β h-eudesman-12, 6α-olide)， 15-acetoxy-11 β h-macrolide-1 (10) e, 4e-diene-12, 6 α - lactone [15 acetoxy ll β h-germacra-l (10) e, 4e-diene-12, 6 α -olide], l β - hydroxyeucalyptus-4 (15), 11 (13) diene-12, 6 α - lactone [l β -hydroxyeudesm-4 (15), 11 (13) - diene-12, 6 α -olide], 4 α - hydroxy-11 β h-eucalyptus-12, 6 α- lactone (4 α -hydroxy-11 β h-eudesman-12, 6 α -olide), 11 β h-danio-l (10) e, 4e diene-12, 6 α lactone [11 β h-germacra-l (10) e, 4E-diene-12，6α-olide]， 15-hydroxy-11 β h-macrolide-l (10) e, 4e-diene-12, 6 α - lactone [15 hydroxy ll β h-germacra-1 (10) e, 4e-diene-12, 6 α -olide], 15 acetoxy macrolide-l (10) e, 4e, 11 (13) - triene-12, 6 α - lactone [15 acetoxygermacra-1 (10) e, 4e, 11 (13) -triene-12, 6α-olide〕, Dahurian-1 (10) e, 4e, 11 (13) - triene-12,6 α - lactone [germacra-1 (10) e, 4e, 11 (13) -triene-12,6 α -olide], dahurian-1 (10) e, 4e, 11 (b) - triene-12,6 α - lactone [germacra-1 (10) e, 4e, 11 (b) -triene-12,6 α -olide], and kaempferol (vladinol) a-f.
3、 Pharmacological effects of Radix Aucklandiae
1. Bacteriostasis and liver protection
Aucklandia has antibacterial effect, can inhibit the regeneration of gastrointestinal bacteria, but also inhibit the replication and propagation of liver virus, and plays the role of anti-inflammatory and liver protection.
2. Invigorate Qi and relieve pain. It is used for epigastric distension and pain caused by qi stagnation in the spleen and stomach, and vomiting due to lack of food.
3. Regulate qi and soothe liver. For hypochondriac pain caused by liver and gallbladder qi stagnation. It has a good therapeutic effect on hepatitis and liver cirrhosis.
4. Invigorate the spleen and eliminate stagnation. Wood incense can regulate gastrointestinal stagnation. Treat abdominal pain, diarrhea and tenesmus.
5. . Yunmu perfume decoction has slight inhibitory effect on Bacillus paratyphi A in vitro, but no inhibitory effect on Shigella, Pseudomonas aeruginosa, Staphylococcus, Streptococcus, etc. It has inhibitory effect on some pathogenic fungi, but has no obvious inhibition on Cryptococcus neoformans. In vitro test also showed a weak inhibitory effect on Trichomonas vaginalis.
6. . Aucklandia not only can reduce blood pressure, but also has a slight diuretic effect.