Honeysuckle
Shanyinhua
LONICERAE FLOS

      This product is the dried flower bud or the flower with initial opening of Lonicera macranthoides hand.-mazz., Lonicera hypoglauca miq., Lonicera confusa dc., or Lonicera fulvotomentosa Hsu et s.c.cheng, a plant of the family Lonicerae. Harvest and dry the flowers before opening in summer.
      [character] Lonicera macranthoides is rod-shaped and slightly curved, 3-4.5cm long, with a diameter of 2mm at the upper part and 1mm at the lower part. Its surface is yellow or yellowish green. The total pedicels are clustered, and the opened corolla lobes are less than half of the full length. The quality is slightly hard, and the hand is slightly elastic. The smell is light, and the taste is slightly bitter and sweet.
      Lonicera glandularis is 2.5 ～ 4.5cm long and 0.8 ～ 2mm in diameter. Its surface is yellowish white to yellowish brown, hairless or sparsely hairy, the calyx tube is hairless, the apex is 5-lobed, the lobes are long triangular, hairy, the lower lip of the Corolla is inverted, and the style is hairless.
      Lonicera japonica Thunb. Is 1.6 ～ 3.5cm long and 0.5 ～ 2mm in diameter. The calyx tube and corolla are densely covered with gray white hairs.
      Lonicera fulva is 1 ～ 3.4cm long and 1.5 ～ 2mm in diameter. The corolla surface is yellowish brown or yellowish brown, densely covered with yellow hairs.
      [identification] (1) surface preparation of this product: the glandular hair of Lonicera macranthoides is less, the head is mostly disc-shaped, the top is flat or slightly concave, and the side view is 5-16 cells, with a diameter of 37-228 μ m; The stem has 2 ～ 5 cells, and the junction with the head is often 2 (～ 3) cells, 32 ～ 240 μ m in length and 15 ～ 51 μ m in diameter. There are many thick walled non glandular hairs, single-cell, horn like, most of which are very short, 21 ～ 240 (～ 315) μ m long, with slightly warty protrusions on the surface, some of which can be seen with threads, and the body cavity is not obvious in the case of short horn; The base is slightly enlarged and triangular. . Pollen grains, 54-82 μ m in diameter.
      The glandular hairs of Lonicera hypoglauca are numerous, and the head is shield shaped and large, with 8-40 cells on the top and 7-10 cells on the side; The stalk is 1-4 cells, extremely short and 5-56 μ m long. The thick walled non glandular hairs vary greatly in length, ranging from 38 to 1408 μ m in length, with fine verrucous processes on the surface and calcium oxalate crystals in some cell cavities.
      There are many glandular hairs in Lonicera japonica Thunb. The head is inverted conical or disc-shaped, and 20-60-100 cells are seen from the side; The stalk has 2-4 cells and is 50-176 (~ 248) μ m long. Thick walled non glandular hairs, single-cell, 32 ~ 623 (~ 848) μ m long, with fine warty protrusions on the surface, some with threads, and wavy horny protrusions on the edge.
      There are two types of glandular hairs of Lonicera fulva: one is relatively long, with an inverted conical or obovate head, 12-25 cells on the side, slightly curved handle, 3-5 (~ 6) cells, 88-470 μ m long; . Thick walled non glandular hairs are straight or slightly curved, 33-2000 μ m long, with sparse warty protrusions on the surface, and some with thin septa.
      (2) . In addition, take chlorogenic acid reference substance and add methanol to make a solution containing 1mg per 1ml as the reference substance solution. Test according to thin-layer chromatography (general rule 0502), suck 10 ～ 20 μ l of the test solution and 10 μ l of the control solution, respectively dot them on the same silica gel h thin-layer plate, use the upper solution of butyl acetate formic acid water (7:2.5:2.5) as the developing agent, develop, take out, dry, and view under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color appear at the corresponding positions of the chromatogram of the control sample.
      [inspection] the moisture content shall not exceed 15.0% (the second method of general rule 0832).
      .
      Acid insoluble ash content shall not exceed 3.0% (general rule 2302).
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; Use acetonitrile as mobile phase A and 0.4% acetic acid solution as mobile phase B, and perform gradient elution according to the following table; Chlorogenic acid was detected at 330nm, and saponin was detected by evaporative light scattering detector. The number of theoretical plates shall not be less than 1000 according to the peak of chlorogenic acid.
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      Time (min) mobile phase a (%) mobile phase B (%)
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    0~10       11.5→15      88.5→85
    10~12      15→29        85→71
    12~18      29→33        71→67
    18~30      33→45        67→55
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      Preparation of reference solution take chlorogenic acid reference, Lonicera macranthoides saponin B reference, Dipsacus saponin B reference at the appropriate location, accurately weigh, add 50% methanol to make a mixed solution containing 0.5mg of chlorogenic acid, 0.6mg of Lonicera macranthoides saponin B, 0.2mg of Dipsacus saponin B per 1ml.
      .
      The determination method accurately aspirates 2 μ l of the reference solution and 5 ～ 10 μ l of 10 μ l of the test solution. Inject the liquid-phase color analyzer to determine the content of chlorogenic acid, and calculate the content of Lonicera macranthoides B and Dipsacus asperoides B with the external standard two-point method and the logarithm equation.
      This product is calculated as dry product and contains chlorogenic acid (c₁₆H₁₈O₉）Not less than 2.0%, containing Lonicera macranthoides B (c₆₅H₁₀₆O₃₂）₅₃H₈₆O₂₂）The total amount of shall not be less than 5.0%.
      [nature, taste and meridian tropism] sweet, cold. Return to lung, heart and stomach channels.
      [functions and indications] clear away heat and detoxify, and evacuate wind heat. It is used for carbuncle, swelling and sore, throat obstruction, erysipelas, heat toxin and blood dysentery, wind heat and cold, febrile disease and fever.
      [usage and dosage] 6 ～ 15g.
      .