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Home > Literature List > Quality evaluation of Polygonum multiflorum in China based on HPLC analysis of hydrophilic bioactive compounds and chemometrics

Quality evaluation of Polygonum multiflorum in China based on HPLC analysis of hydrophilic bioactive compounds and chemometrics

Journal name:Journal of Pharmaceutical and Biomedical Analysis
Literature No.:
Literature Url: http://www.sciencedirect.com/science/article/pii/S0731708512004852
Date publication:18 January 2013

Quality evaluation of Polygonum multiflorum in China based on HPLC analysis of hydrophilic bioactive compounds and chemometrics

D.Q. Han, J. Zhao , J. Xu, H.S. Peng, X.J. Chen, S.P. Li , 

Abstract

Polygonum multiflorum is one of the most commonly used Chinese medicines. In this study, an effective pressurized water extraction and HPLC method was developed for first simultaneous determination of 8 hydrophilic compounds, including gallic acid, Hypaphorine, Catechin, Proanthocyanidin B1, Epicatechin, Proanthocyanidin B2, Emodin-8-O-β-d-glucopyranoside, stilbene glycosides, in P. multiflorum. The analysis was performed on a Zorbax SB-AQ column with gradient elution of 0.05% phosphoric acid aqueous solution and acetonitrile in 45 min. All calibration curves showed good linearity (R2 > 0.9994) within test ranges. The LOD and LOQ were lower than 0.2 and 1.0 μg/mL on column, respectively. RSD for intra- and inter-day of 8 analytes were less than 4.1% and 4.0%, respectively, and the overall recovery was 96.0–100.7%. The validated method was successfully applied to quantification of 8 hydrophilic compounds in samples of P. multiflorum from different locations of China. Chemometrics such as principal component analysis (PCA) and 

 Proanthocyanidin B1 (>95%) and B2 (>95%) were purchased from Chengdu
Biopurify Phytochemicals Co. Ltd. (Chengdu, China).