【Write in front】：How to determine the animal dose of traditional Chinese medicine and monomers is a problem often encountered by researchers in the study of drug action and mechanism, including manuscript reviewersThis article makes a simple exposition on this issue, and welcome criticism and correction if there is anything inappropriate. Thank you.
1.【Inter animal dose conversion table】
This table is in theLaboratory zoologyIt is mentioned in this book, which is also what we need to know in advance before we start today's topic (see the table below). The table may be slightly different from different sources, but we estimate that the dose does not affect.
To give a simple example:
If the gavage dose of human is 100mg/kg, it can be converted into the dose of rat:Note here thatFirst look at the left column in the table and then look at the row, soThe conversion value is 0.018. The（Generally, the smaller the animal is, the higher the equivalent dose will be. Don't be wrong）
Table of equivalent dose ratio converted by body surface area between humans and animals(from douding.com)
It is also convenient for others to simply convert to equivalent multiples:
2.【How to determine the intragastric dose】
. For example, Gegen Qinlian decoction, a traditional Chinese medicine compound, we can find the intragastric dose in the literature, for example:
As mentioned in the methods of animal modeling and gavage:
If there is in the literature, we can quote the dose, but it may not be applicable. thatWe can check the Chinese Pharmacopoeia to see the human dose, or check the clinical dosage. According to the results, we can initially determine the human dose of Gegen Qinlian decoction, and then convert it according to the above scale。
After determining this initial dose, we need toPre experimentAnimals were gavaged,Observe the morphological changes of animals under the gavage dose within 7-14 days, and set different gradients, and finally find a maximum non-toxic concentration or doseThat is, at the maximum non-toxic concentration, animal gavage is safe and will not affect the experimental results. In this way, we can choose the dose between the initial concentration and the maximum non-toxic concentration. Generally, the initial dose will be far less than the maximum non-toxic concentration.
For example, Gegen Qinlian decoction can learn from Gegen Qinlian tablets in the Pharmacopoeia. For adults, it is about 1.0g a day. If mice are gavaged, it is about 10g/kg a day after conversion. For 20g mice, it needs 0.2g a dayGegen Qinlian Decoction extract or extract。
3.【About the setting of high, medium and low dose groups】
In fact, there are no exact requirements and standards for high, medium and low doses, can1:2:4perhaps1:3:9Similar ratiosAnd then within the maximum non-toxic concentration range. As mentioned in the above article, it is set in a 1:3:9 manner.
