The development of medicine is inseparable from basic and applied research, and the detection reagent for scientific research is one of the most important scientific research tools. Scientific research detection reagents mainly include protein, antibody and detection kit, and the development of kit is based on protein and antibody. .

At present, as one of the basic scientific research tools, the level of chaos in the industry does not match the rigorous requirements of scientific research experiments. "The detection reagent industry for scientific research belongs to an emerging industry and is at the forefront of science, so it is difficult to form a unified industry standard, resulting in a few manufacturers fishing in troubled waters." Wuhan cloud clone once described in the prospectus.

In fact, the whole industry felt helpless about the low effectiveness of scientific research and detection reagents. Some manufacturers imported scientific research kits to fabricate by replacing indicators and selling both true and false..

How chaotic is the market of scientific research reagents?

There is such a case: the human protein atlas (HPA) program is a scientific research project initiated by Sweden to describe the characteristics of all human proteins. Because this project uses a large number of antibodies, in order to ensure the accuracy of the experimental results, researchers verified more than 9000 antibodies (including monoclonal antibodies and polyclonal antibodies) from 29 suppliers to detect whether these antibodies can work effectively.

The test results were surprising:

49% of the antibodies were completely ineffective, 29% of the antibodies were uncertain, and only 22% of the antibodies were considered to be supportive of the experimental data. What is more surprising is that only 7% of the antibodies are considered to be completely effective and have pre - and post experimental consistency.

Not only the international environment, but also the domestic market situation of scientific research and detection reagents is not optimistic. There are many kinds of domestic scientific research reagent market, with both good and bad merchants; The purchase is scattered, but the total consumption is huge. .

What is more troubling for researchers is that before drugs are launched, pharmaceutical companies often need to go through strict clinical trials to verify whether the products are effective, but antibody products do not have such requirements, and even the antibodies used by researchers are difficult to verify the quality.

At present, China has no special legislation on the detection reagent industry for scientific research, and there is no special government department to supervise it. There is also no unified regulatory system in the industry.

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How to identify fake reagents?

First of all, don't be greedy for cheap

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This section takes ELISA kit as an example. Because of the characteristics of simple experiment, few operation steps, one-time experiment and so on, ELISA reagent is also considered by the industry to be the most vulnerable reagent to fake. The key points of identification after the arrival of goods are as follows:

1. From the package

Fake goods: No manufacturer's name, telephone number, address and other information; The cost of outer packing box is relatively expensive;

2. Experimental identification

The interference experiment can be used to distinguish whether the ELISA Kit detects the target antigen. The method is as follows:
(1) The recombinant protein antigen specific to the kit and the detection antibody in the kit were configured into a mixed incubation solution with antibody: antigen ≈ 1:2
(2) After incubation at 37 ℃ for 15 min, the detection antibody in the original kit was replaced
(3) The follow-up operation was carried out according to the kit instructions, and the detection antibody, enzyme complex and substrate were added
(4) .

(5) .
In addition, if two ELISA kits a and B from the same company are purchased, cross experiment can also be used to identify whether the ELISA kit is fake.

3. Other identification methods

Psychological Tactics:

If you call the so-called R & D or technology of a counterfeit business and say that all reagents are to be tested, he may also feel guilty and afraid.

According to the contract:

When purchasing products, sign a formal contract and carefully review the terms of the contract. If the remittance account in the contract is a personal account, it is recommended to keep an eye on it. It is recommended that the other party provide a company account.

Finally, we hope that at the same time of self-defense, the relevant departments can work together to purify the scientific research environment, improve the legal system, and strive to innovate to help scientific research, so as not to let those who sell dog meat with sheep's heads, fake imported reagents, and those who have no integrity at all, and make fake and fake things run amok.

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