Guizhi Fuling prescription consists of Guizhi, Fuling, peony bark, white peony, peach kernel5Flavor and drug composition. Among them, Ramulus Cinnamomi takes its effect of warming the meridians and activating blood stasis as the monarch medicine, peach kernel and its compatibilizer as the minister medicine, and poria cocos, peony bark and Paeonia Alba as the adjuvant medicine[1]As a pill, it was first published in Zhang Zhongjing's synopsis of the Golden Chamber•Women's pregnancy "is used to treat women who have been known to have lump, causing restless fetal movement or missing more than one syndrome. The compendium of economic classics changed it into a decoction for inducing labor. In modern clinical practice, it is used to treat dysmenorrhea, pelvic inflammatory disease, uterine fibroids and other gynecological diseases. However, with the continuous progress of clinical medicine, this prescription has also shown good curative effect on other clinical diseases, so the research reports on this drug at home and abroad have been increasing in recent years. This paper reviews the research progress of Guizhi Fuling prescription in various aspects in recent years（Q-marker）"Five Principles" for Guizhi Fuling prescriptionQ-markerIn order to provide references for the quality control research of Guizhi Fuling prescription, the prediction analysis was carried out.
1 Main chemical composition
The ingredients of Guizhi Fuling prescription are complex. At present, most studies focus on pharmacological effects, clinical applications, chemical components of single herbs or the isolation and identification of several main effective components in the compound[2-7]However, as an organic whole, it is unreasonable to clarify the material basis and use it for quality control by simply adding the medicine flavor. Previous studies have systematically studied the chemical constituents of Guizhi Fuling compound[8-9], through the treatment of Guizhi Fuling capsules（GZFLC）80Multiple compounds. These compounds are mainly monoterpenes and their glycosides, such as paeoniflorinpaeonidanin A, paeoniflorin, Hyperoside, paeoniflorinR1, sodium sulfonate paeoniflorin, etc; C, dehydrotomolic acid, betulinic acid, oleanolic acid, caroteneβ-Sitosterol, Ivy saponin, etc; Flavonoids, such as apigenin, catechin, epicatechin, quercetin, etc; Organic acids, such as benzoic acid, p-coumaric acid, vanillic acid, gallic acid, trans-o-methoxy cinnamic acid, etc; Phenols and phenolic glycosides, such as paeonol and paeoniflorinF, paeoniflorinA, paeoniflorinCIn addition to the above ingredients, there are glycosides, amino acids, sterols, sugars, lignans, coumarins and some other ingredients, such as rosarin, amygdalin, leucine, galactitol, campesterol, ethyl gallate, vanillin, coumarin, eugenol, eugenol, cinnamol, etc. Studies have proved that paeonol is an effective component in the treatment of endometriosis[10]Quercetin and catechin can be used in the treatment of cancer[11-12]. Liweisi et al[13]adoptHPLC-ESI-QTOF/MSThe chemical constituents of Guizhi Fuling capsules were isolated and identified18Chemical constituents, mainly paeoniflorin compounds, are gallic acid, paeoniflorin, paeoniflorin sulfite, hydroxypaeoniflorin, paeoniflorin glycoside, butyric acid, benzoyl oxidized paeoniflorin, paeoniflorinC, benzoyl paeoniflorin, paeoniflorinAandO-β-D-gentiobiosyl-D-(−)-mandelamideand (2R)-[6-O-β-D-glucopyranosyl oxy] (phenyl) ethanoic acidSome studies have established the Guizhi Fuling CapsuleHPLCFingerprints and qualitative analysis of its characteristic peaks identified8The chemical constituents are gallic acid, paeoniflorin, paeoniflorin1,2,3,4,6-β-Pentagalloyl glucose, cinnamic acid, benzoyl paeoniflorin, cinnamaldehyde and paeonol. Lin Xia et al[14]Triterpenoids from Guizhi Fuling capsules were establishedUPLCFingerprints, andUPLC-Q-TOF/MSPreliminarily determined15Four components, namely, Ivy saponin, daucosterol, tumoic acid and poria acidD, dehydrotomolic acid, Polyporus acidC、3-Table dehydrotomolic acid, dehydrocarbaric acidα-Linolenic acid, oleanolic acid, Poria acid, linoleic acid, methyl palmitoleate, palmitic acid and ethyl palmitate.
2 pharmacological action
2.1 Immunomodulatory effect
In Guizhi Fuling prescription5The traditional Chinese medicine and many of its components have immunoregulatory effects. Zhang Haiqin et al[15-16]Studies have shown that Guizhi Fuling capsules can regulateTCell subsets and the role of erythrocyte immune function. It was found that Guizhi Fuling pills can change the tumor bearing miceTlymphocyteFas, and by decreasing theFasExpression to inhibitTApoptosis of lymphocytes, thereby effectively regulating the body's immune function, which is also through the regulation of immune function to treat cervical cancer. [17]The anti-inflammatory and immunomodulatory active components of Guizhi Fuling capsules were studied by using high content technology, and finally selected15The active ingredients are gallic acid, paeoniflorin, paeonol, amygdalin, benzoyl paeoniflorin, pentagalloylglucose, cinnamaldehyde, dehydrochoric acid, Poria polysaccharide, quercetin, coumarin, cinnamic acid, Poria acid, paeoniflorin, dehydrotomolic acid. [18]Guizhi Fuling capsules and its15The results showed that Guizhi Fuling Capsule and the active ingredient composition had immunomodulatory effect, which may be through enhancing the activity of spleen lymphocytes, promotingTLymphocyte activating moleculeCD69andCD25Expression of as well as costimulatory moleculesCD80andCD86To improve the occurrence of immune response and play a role in regulating immune function.
2.2 antitumor activity
Guizhi Fuling decoction can inhibit tumor growth and metastasis and prolong the survival time of patients[19]. research[20]It was found that Guizhi Fuling pill could inhibit tumor growth, and it was thought that its anti-tumor mechanism might be through inhibiting mutantP53Proteins and cell adhesion moleculesCD44To affect the proliferation cycle of tumor cells and the process of cell adhesion, degradation and migration, so as to achieve the purpose of anti-tumor. There are also studies[21]Showed that Guizhi Fuling pills could reduce theS180Abnormal increase of vascular endothelial growth factor in serum of tumor bearing mice（VEGF）Levels, increasing tumor metastasis suppressor genes in tumor cellsnm23H1, which reduces adhesion molecules on tumor cellsCD44To inhibit tumor metastasis. research[22]Showed that the tumor inhibition rate of Guizhi Fuling pills was38.93%， The apoptosis rate was17.79%, the results showed that Guizhi Fuling pill was through the up regulation ofp21waf/cipProtein expression to inhibit cyclin dependent enzyme activity and proliferating cell nuclear antigen（PCNA）Activity, thereby blocking theDNASynthesis and replication, inhibition of tumor cell proliferation and survivin（survivin）mRNAExpression, promote apoptosis, and then achieve the purpose of inhibiting tumor growth. Fanqiru et al[23]Apply molecular docking method to virtual screening13The active ingredients of geguizhi Fuling capsules against uterine fibroids are mainly catechin, quercetin, amygdalin, paeonol, gallic acid, benzoyl hydroxypaeoniflorin, o-phenylmethyl cinnamaldehyde1,2,3,6-O-Tetragalloylglucose et al. While there are studies[24]The mechanism of Guizhi Fuling Capsule in the treatment of uterine fibroids may be related to the reduction of estradiol and epidermal growth factor（EGF）The level of correlation.
2.3 anti-inflammatory effect
It is reported in the literature[25-26]Guizhi Fuling decoction has anti-inflammatory effect. Wuxiuhong et al[27]Studies have shown that Guizhi Fuling pill can significantly reduce the degree of xylene induced ear swelling in mice, which proves that it has a good anti-inflammatory effect. Cheng Yue et al[28]The study found that Guizhi Fuling capsules and15All the active ingredient compositions have obvious anti pelvic inflammatory effect in rats, and it is believed that this15The three active ingredients may be the material basis of Guizhi Fuling Capsule for anti pelvic inflammatory disease. Zhang Zhenzhen et al[29]Guizhi Fuling capsules and its15（LPS）Induced interleukin-1β（IL-1β）, tumor necrosis factor-α（TNF-α）E2（PGE2）And all of them showed a certain concentration-Effect dependence; IL-1βMembrane bound prostaglandinsE2Synthases（mPGES-1）Expression of. IL-1βWithmPGES-1Is related to the expression of. Tangxingmei et al[30]It was found that Guizhi Fuling Capsule and its active ingredients can inhibit inducible nitric oxide synthase（iNOS）-2（COX-2）Protein expression, which in turn inhibits inflammatory mediatorsNOandPGE2, while inhibiting inflammatory factorsIL-1β、IL-6andTNF-αTo exert anti-inflammatory effects.
2.4 Analgesic effect
Guizhi Fuling prescription has good analgesic effect, especially the pain caused by dysmenorrhea, and the analgesic effect is obvious[31-32]. sun LAN et al[33]Observation on the effect of Guizhi Fuling Capsule on prostaglandinsF2α（PGF2α）Mouse dysmenorrhea model and oxytocin（OT）It was found that Guizhi Fuling Capsule can significantly reduce the number of writhing in rats and mice and reduce the uterine tissue of ratsPGF2αContent andPGF2α/PGE2Values, elevated in mouse serumβ-Endorphins（β-EP）、6-ketone-prostaglandinF1α（6-keto-PGF1α）Content. It shows that Guizhi Fuling Capsule has the effect of treating dysmenorrhea, and its mechanism and regulation in vivoβ-EP、6-keto-PGF1α、PGF2α、PGE2Level related. [34]Studies have shown that Guizhi Fuling capsules and their composition can inhibitPGF2αInduced contraction of uterine smooth muscle and decreased uterine tissuep42/44MAPKPhosphorylation level, inhibitionCx43Protein expression.
Zhongyunxi et al[35]It was found that paeonol in Guizhi Fuling capsules can significantly inhibit the swelling of rat toes, inhibit pain, inhibit endometrial hyperplasia, and correct superoxide dismutase（SOD）、NO, malondialdehyde（MDA）Abnormal expression of. The effect of paeoniflorin is similar to that of paeonol, but the effect of paeoniflorin is weaker than that of paeonol. Guizhi Fuling Capsule may treat primary dysmenorrhea by inhibiting the expression of inflammatory mediators by paeonol. [36]Based on network pharmacology, the molecular mechanism of Guizhi Fuling Capsule in the treatment of dysmenorrhea, uterine fibroids and pelvic inflammatory disease was studied. It was found that the pentacyclic triterpenoids and sterols in Guizhi Fuling Capsule, such as dehydrochoric acid, dehydrotomolic acid, citrostadienol, and xanthone quercetin, could interact with multiple target proteins, inhibit the contraction and proliferation of uterine smooth muscle, and inhibit the inflammatory response.
2.5 Other roles
Guizhi Fuling formula can also reduce blood viscosity, improve blood pressure, antioxidation, and improve cerebral ischemia-Reperfusion injury, etc. There are studies[37]It was found that the extract of Guizhi Fuling prescription could relieve sodium nitroprusside（NODonor) - mediated cell death as well as decreased levels ofNOTo inhibit theNOSynthetase to reduce ischemia-Reperfusion injury, indicating that Guizhi Fuling Capsule can inhibit cerebral ischemia-Lesions brought about by reperfusion. It has also been shown that Guizhi Fuling Capsule can reduce cerebral ischemia-The volume and water content of cerebral infarction in rats after reperfusion, and the effect on cerebral ischemia-Reperfusion injury has a protective effect.Zhangetc.[38]Studies have shown that Guizhi Fuling pill can significantly promote the recovery of nervous system and reduce the infarct size in rats with middle cerebral artery infarction, and its mechanism of neuroprotection may be related to the anti-inflammatory effect.
3 clinical application
3.1 Gynecological diseases
Guizhi Fuling formula is most widely used in gynecological diseases, such as uterine fibroids, ovarian cysts, chronic pelvic inflammatory disease, endometriosis, dysmenorrhea and so on. Hanzhaozhong et al[39-40]Research shows that Guizhi Fuling Capsule Combined with mifepristone has a synergistic effect in the treatment of uterine fibroids, can improve the clinical total effective rate, and has the advantages of good long-term efficacy and less adverse reactions. Zhaoying[41]Guizhi Fuling capsules combined with mifepristone in the treatment of uterine fibroids50Cases, clinically cured27Cases, markedly effective13Example, effective10E.g., total effective rate100%. Liu Gang et al[42]The clinical efficacy of Danggui Shaoyao Powder Combined with Guizhi Fuling pills in the treatment of simple ovarian cysts was studied120Patients with pure ovarian cysts were randomly divided into Danggui Shaoyao Powder group, Guizhi Fuling pill group and Danggui Shaoyao Powder Combined with Guizhi Fuling pill group3After months of treatment, it was found that the clinical efficacy of Danggui Shaoyao Powder Combined with Guizhi Fuling pill group was better than that of other groups, which could effectively improve the symptoms of patients. Lichunxiang et al[43]Treatment of chronic pelvic inflammatory disease with Guizhi Fuling pills assisted by antibiotics104Examples, of which52Cases were treated with conventional antibiotics52The study found that Guizhi Fuling pill assisted antibiotic treatment can significantly reduce the inflammatory state of patients, improve the hemorheology and clinical symptoms of patients, and the treatment effect is significant and safe. Zongxiao[44]The research shows that Guizhi Fuling pill has exact clinical effect in the treatment of endometriosis, and can improve the clinical symptoms and biochemical indicators of patients. There are studies on the effect of the use of the method of "the" of "the" of "the" of "the" of "the" of "the" of "the" of "the" of "the" of "the" of[45-47]The results showed that Guizhi Fuling decoction combined with chemical drugs in the treatment of endometriosis could effectively improve the total effective rate of clinical treatment, reduce the recurrence rate and have higher safety than chemical drugs alone. Wang Jia[48]Treatment of endometriosis with Guizhi Fuling Capsule Combined with mifepristone88Cases were divided into single drug group (mifepristone) and combination group (mifepristone and Guizhi Fuling capsules)2Group, in which patients in the combination group were cured11Example: remarkable effect22Example: effective9Example: invalid2Cases, clinical total effective rate95.5%, compared with the total clinical effective rate of the single drug group81.8%The clinical curative effect is more significant.
3.2 other
In addition to gynecological diseases, Guizhi Fuling formula can also treat diseases of reproductive system, cardiovascular system, digestive system, nervous system, etc., such as prostatic hyperplasia, coronary heart disease, angina pectoris, liver cysts, liver cirrhosis, cerebral infarction, etc. Sunyiming et al[49]Treatment of benign prostatic hyperplasia with Guizhi Fuling pill modified decoction combined with Acupoint Injection50Cases, it was found that it can effectively improve the treatment efficiency and clinical symptoms. Lu Jin et al[50]Treatment of chronic heart failure patients with kidney yang deficiency syndrome with Zhenwu Decoction and Guizhi Fuling pills60Cases were divided into conventional chemical drug group and combined drug group, in which the treatment rate of combined drug group was89.7%, compared with the conventional chemical drug group（71.4%）. Hongsheng Wang[51]Treatment of cerebral infarction patients with Guizhi Fuling pill plus Astragalus membranaceus and chemical drugs30After observing the changes of symptoms before and after treatment, it was found that Guizhi Fuling pill plus Astragalus membranaceus and chemical drugs can improve the therapeutic effect of cerebral infarction.
4 quality control
4.1 Determination of index components
Guizhi Fuling prescription has many dosage forms because of its remarkable clinical effect. Most of its quality control research focuses on the determination of a variety of effective ingredients. Liuguohong et al[52]adoptHPLCThe contents of amygdalin and paeoniflorin in the effective part of Guizhi Fuling pills were determined. The results showed that there was a good linear relationship within the linear range, and the average recovery was98.48%、99.06%The method is specific and simple, and can be used for the quality control of the effective parts in Guizhi Fuling pills. Lizhihui et al[53]adoptHPLCThe contents of Paeonol and paeoniflorin in Guizhi Fuling pills were determined. The results showed that both showed a good linear relationship within the linear range, and the average recoveries were99.5%、99.4%, precision and repeatabilityRSDAverage ＜1.5%The method is simple and accurate for the simultaneous determination of Paeonol and paeoniflorin in Guizhi Fuling pills, and can be used for the quality control of Guizhi Fuling pills. [54]useHPLCThe contents of dehydrotomolic acid and grifolic acid in Guizhi Fuling capsules were determinedC、3-Epidesmoparacic acid and desmoparacic acid4Triterpenoid acids from Poria cocos, results4The average recovery was97.5%、98.5%、97.2%、102.3%The established method is feasible and reproducible, and can be used for the quality control of Guizhi Fuling capsules. Yin Quanwei et al[55]adoptHPLCThe content of dingguizhi Fuling capsules was determinedα-Content of linolenic acid, linoleic acid and oleic acid, results3The average recovery was97.02%，97.17%，96.98%The method has good precision, repeatability and stability, and is suitable for the determination of active ingredients in Guizhi Fuling capsules, which can provide reference for quality control.
4.2 Fingerprint
《Chinese Pharmacopoeia》2015Annual Edition[56]On the basis of the content determination of multi index components, the fingerprint of Guizhi Fuling capsules was addedTLCMethod combined with microscopic identification was used for quality control and evaluation of Guizhi Fuling capsules. Wuxiuhong et al[57]establishThe fingerprint of Guizhi Fuling pills was analyzed by the similarity evaluation system software of chromatographic fingerprint of traditional Chinese medicine. It was considered that the method was characteristic and exclusive, and could be used for the quality control of Guizhi Fuling pills. [58]The liposoluble components of Guizhi Fuling pills were establishedGC-MSAnd identified the9After the analysis of similarity evaluation system software,10The similarity of samples from batches was within0.90Above, it is believed that the atlas is highly characteristic and exclusive, and can be used for the quality control of the fat soluble components of Guizhi Fuling pills. Lin Xia et al[14]A method for the determination of triterpenoids in Guizhi Fuling capsules was establishedUPLCFingerprints, identified20Common peaks,10The similarity of finished products in batches is0.90Above, it is considered that the precision, repeatability and stability of the chromatogram are good, which can be used for the quality control of Guizhi Fuling capsules.
4.3 Other methods
Modern mass spectrometry technology is widely used in the research of traditional Chinese medicine due to its advantages of high sensitivity, high selectivity and rapidity. Wangshuyun et al[59]adoptGC-MSThe volatile oil components of Guizhi Fuling capsules and its components were analyzed, identified and compared. It was found that the volatile oil component of Guizhi Fuling capsules was mainly benzoin aldehyde（benzaldehyde）Paeonol（paeonol）. adoptHPLC-DAD-ESI/MSThe fingerprint characteristic peaks of Guizhi Fuling capsules were identified by the combined technology, and finally8Components and a terpenoid compound were speculated, which had good reproducibility and characteristics, and could be used for the quality control of Guizhi Fuling capsules.
5 Q-markerforecast
Q-markerIs academician liuchangxiao[60]The new concept proposed refers to the marked substances that are inherent in traditional Chinese medicine and traditional Chinese medicine products or formed during processing and preparation, are closely related to the functional attributes of traditional Chinese medicine, and can reflect the quality and efficacy of traditional Chinese medicine.
5.1 Based on quality transfer and traceabilityQ-markerPredictive analysis
Mass spectrometry was performed from5It was identified in the herbal medicine156Compounds[61-63], guizhizhong16Compounds, including4Aldehydes6Organic acids2Alcohols1Ketones2Esters1Coumarins; 35Compounds, including34Triterpenoids1Other classes; 38Compounds, including21Monoterpenes and their glycosides4Phenolic glycosides6Gallic acid glucose3Organic acids4Other classes; Peony bark48Compounds, including11Gallic acyls17Monoterpenes and their glycosides8Phenolic acids1Triterpenoids7Acetophenones and4; Taorenzhong19Compounds, including2Glycosides8Sterols9Fatty acids. The chemical constituents of Guizhi Fuling capsules were analyzed and identified78Compounds: monoterpenes and their glycosides16Triterpenoids and their glycosides10Flavonoids7Individual, phenols and phenolic glycosides3Organic acids11Alcohol2Pieces, sugars4Individual, galloylglucose1Sterols5Individual, coumarins2Amino acids4Individual, lignans1Individual, glycosides3Triterpenoids, diterpenes and their glycosides1PCs. aldehydes1Pieces, adenosine2PCs. other classes5PCs. adoptUPLC/Q-TOF-MSAnalysis of blood components in Guizhi Fuling capsules by HPLC[64], identified from14Ingredients, including13Prototypical components (monoterpenes and their glycosides3Individual, phenols and phenolic glycosides2Triterpenoids and their glycosides3Organic acids3Individual, galloylglucose1And other classes1And1According to the research, the wild cherry glycoside, a metabolite, should be formed by amygdalin through the action of intestinal flora.
5.2 Based on component specificityQ-markerPredictive analysis
Ramulus Cinnamomi is Cinnamomum cinnamomi, a plant of Cinnamomum in LauraceaeCinnamomum cassiaPreslAccording to literature research, cinnamon twigs mainly contain volatile oil components, mainly aldehydes, mainly cinnamaldehyde, myristicaldehyde, o-methoxycinnamaldehyde, etc. In addition, there are cinnamic acid, cinnamyl alcohol, coumarin and other organic acids, coumarins, terpenoids and other components. Ranoxane diterpenes are characteristic constituents of camphor plants in Lauraceae[65]。
Poria cocos is a Polyporaceae fungus Poria cocosPoria cocos (Schw.) WolfAccording to literature research, Poria cocos mainly contains triterpenoids, polysaccharides, diterpenes, sterols and other components. .
Paeonia lactiflora Pall Paeonia lactiflora Pall. The dried root of paeoniflorin mainly contains monoterpenes such as paeoniflorin, paeoniflorin and catechin and their glycosides, triterpenes, polyphenols, flavonoids and other components. Monoterpenes and their glycosides as well as polyphenols are the main active ingredients of Paeonia lactiflora, which have a variety of pharmacological effects, among which monoterpenes and their glycosides are more characteristic.
Peony bark is a Ranunculaceae plant PeonyPaeonia suffruticosa Andr. The dried root bark of paeonol mainly contains monoterpenoids and their glycosides, phenols and phenolic glycosides, triterpenoids and their glycosides, among which the phenolic and phenolic glycosides, mainly paeonol, are the main components. It not only has high content, but also has the effects of regulating immunity, inhibiting central reaction, analgesia, etc.
Peach kernel is a rose plant peachPrunus persica (L.) BatschOr ShantaoP. davidiana(Carr.) Franch. The main components of the dried and mature seeds are fatty acids and glycosides, and the glycosides mainly composed of amygdalin are the effective components of peach kernel, but they widely exist in Rosaceae plants with poor specificity. Other components of glycosides, such as methyl mandelate glycoside and fudoside, are more specific.
5.3 Based on the correlation between components and efficacyQ-markerPredictive analysis
According to the network pharmacology analysis, the targets of Guizhi Fuling Capsule in the treatment of uterine fibroids, primary dysmenorrhea and pelvic inflammatory disease are115PCs, biological pathways15Yes, it's clear35Chemical constituents, mainly triterpenoids, flavonoids and sterols. Molecular imprinting technology was used to quantitatively knock out the main components (gallic acid, paeoniflorin, paeonol, etc.) in Guizhi Fuling capsules step by step15Compounds) and observed the effect of changes in their main components on the main efficacy. It was found that the efficacy gradually decreased with the knockout of the components. It was believed that gallic acid, paeoniflorin, paeonol, etc15Compounds are the main pharmacodynamic material basis of Guizhi Fuling capsules. 8, mainly from Ramulus Cinnamomi, Poria cocos, Paeonia alba and peony bark. According to the literature research, the main active ingredients of Guizhi Fuling Capsule, paeoniflorin, paeonol, pentagalloyl glucose, Poria cocos polysaccharide, paeoniflorin, amygdalin, gallic acid and cinnamaldehyde, have played a major role in the treatment of uterine fibroids, primary dysmenorrhea and pelvic inflammatory disease[66-67]。
5.4 Based on the compound compatibility environmentQ-markerPrediction analysis of
Guizhi Fuling Capsule was used to treat chronic pelvic inflammatory disease rat model, uterine fibroids rat modelS180Tumor bearing micePGF2α; It has a therapeutic effect on mice with uterine fibroids, can reduce the uterine coefficient of rats with uterine fibroids, and reduce the proliferation of uterine wall smooth muscle; At the same time, it can inhibit tumor growth and metastasis, prolong survival time, and reduce serumE2Level, suppressionsurvivin mRNAExpression, promoted apoptosis, and had anti-tumor effect; β-EP、6-Keto-PGF1α. The above research shows that Guizhi Fuling Capsule has the effects of regulating immunity, anti inflammation, anti-tumor and analgesia. Construction of Guizhi Fuling capsules based on network pharmacology-molecule-Target network（herb-drug-target network，HDTN）, which contains115Molecules,116Target proteins. 46Molecules from Ramulus Cinnamomi46Molecules are from Poria cocos,28Molecules are from peach kernel,27Molecules are from Radix Paeoniae Alba27The components of Cinnamon Twig and peach kernel were mostly overlapped, which verified the rationality of the prescription. F, paeoniflorin, etc. can interact with multiple target proteins such asNOS, matrix metalloproteinases-9（MMP-9）, glycogen synthase kinase（GSK）, phosphatidylinositol-3-Hydroxykinase（PI3K）To regulate multiple biological channels to reduce inflammatory response, relieve pain and improve disease. Molecular docking method showed that many components in Guizhi Fuling Capsule, such as amygdalin, paeonol, benzoyl paeoniflorin, gallic acid, etc., could interact withER、CDK2、EFGRThe combination of target proteins can achieve the anti-tumor effect by inhibiting cell lesions and proliferation. The results showed that dehydroparacic acid, paeoniflorin, benzoyl paeoniflorin, paeoniflorinF, amygdalin, Paeonol and gallic acid are the main effective ingredients of Guizhi Fuling capsules.
5.5 Component Testability BasedQ-markerPredictive analysis
Simultaneous determination of paeoniflorin, cinnamic acid, cinnamaldehyde, Paeonol and other components in Guizhi Fuling pills by inverse HPLC wavelength conversion method[68]The fingerprint quality control method of Guizhi Fuling capsules was established10The fingerprint of Guizhi Fuling capsules was determined13Shared peaks and assigned them, among which2From Radix Paeoniae Alba3From peony bark2From Ramulus Cinnamomi6From Paeonia alba and peony bark; Triterpenoids from Guizhi Fuling capsules were establishedUPLCFingerprint method, determined the20Common peaks, and their sources were assigned, among which13From Poria cocos,2From Ramulus Cinnamomi,1From white peony and peony bark,1From peach kernel, white peony, peony bark and Cinnamon Twig.
In conclusion, paeoniflorin, paeonol, pachyman, gallic acid, amygdalin and cinnamaldehyde in Guizhi Fuling prescription may be the main pharmacodynamic material basis, and can be used as the quality marker of Guizhi Fuling prescription.
6
Guizhi Fuling prescription is a traditional Chinese medicine compound, which is widely used in the treatment of gynecological diseases. In recent years, there are many kinds of preparations, such as Guizhi Fuling capsules, Guizhi Fuling pills, Guizhi Fuling honey pills, etc. Different kinds of preparations have different quality standards. Based on the review of the research progress of Guizhi Fuling prescription in chemical composition, pharmacological effect, clinical application and other aspects, this paper discusses the effect of Guizhi Fuling prescription based on the "Five Principles"Q-markerAfter analysis, Guizhi Fuling prescriptionQ-markerThe screening provided a reference.
References (omitted)
Come Source: Zhang Liye, Tian Chengwang, Liu Suxiang, Chen Changqing Chemical composition, pharmacological effect and quality marker (q-marker) prediction analysis of Guizhi Fuling prescription [j]. Chinese herbal medicine, 2019, 50 (2): 265-272