1、 Pharmacopoeia standard of Sophora japonica
This product is the dried and mature fruit of sophorajaponica L., a legume. Harvest in winter, remove impurities and dry.
. It is soft, dry and wrinkled, easy to break at the contraction, yellow green section, and viscous. The seeds are 1-6, kidney shaped, about 8 μ m long, with smooth surface, brown black, and gray white round navel on one side; Hard texture, cotyledons 2, yellow green. The flesh is slightly smelly and tastes bitter, and the seeds chew with beany smell.
[identification] (1) the powder of this product is dark gray brown. The surface of pericarp epidermal cells is polygonal, and ring pores can be seen. The palisade cells of seed coat are columnar in lateral view, with thick walls, and the glow zone is located at the apical edge; The top surface is polygonal, and the wall is densely Beaded thickened; The bottom looks like a circle, containing gray brown objects. The seed coat Sertoli cells are dumbbell shaped in lateral view, and some of the cell cavities contain gray brown matter. . The stone cells are rectangular, round, triangular or shell shaped, and the pore groove is obvious.
(2) Take this product and test it according to the method under [content determination]. The chromatographic peak of the test product should be consistent with the retention time of the chromatographic peak of the control product.
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Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol acetonitrile-0.07% phosphoric acid solution (12:20:68); The detection wavelength is 260nm. The number of theoretical plates should not be less than 3000 according to the Sophoricoside peak.
Preparation of reference solution take an appropriate amount of Sophoricoside reference, weigh accurately, and add methanol to make a solution containing 40 μ g per 1ml.
Preparation of test solution take about 2G of powder (passing through No. 3 screen), weigh accurately, place in a corked conical flask, add 50ml of 70% ethanol precisely, weigh, sonicate (power 300W, frequency 25kHz) for 45 minutes, cool, weigh again, use 70% ethanol to make up the lost weight, shake well, and filter. Accurately measure 0.5ml of the filtrate, put it into a 20ml volumetric flask, add methanol to the scale, and shake well.
The determination method is to precisely suck 10 μ l of the reference solution and 10 μ l of the test solution, inject them into the liquid chromatograph, and determine.
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Decoction pieces
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[character], [identification], [content determination] are the same as those of medicinal materials.
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For every 100kg of locust horn, 5kg of refined honey shall be used.
This product is shaped like locust horn, and its surface is slightly raised, yellowish brown to dark brown, shiny, and slightly viscous. It has honey aroma and tastes slightly sweet and bitter.
[identification] the same as medicinal materials.
[content determination] take this product, bake it at 80 ℃ for 1-3 hours, smash it (through No. 3 screen), take about 2G, weigh it accurately, and determine it according to the method under Huaijiao [content determination].
The content of Sophoricoside (c21h20o10) in this product shall not be less than 3.0% according to the calculation of dry product.
. Return to liver and large intestine channels.
[functions and indications] clearing heat and purging fire, cooling blood and stopping bleeding. .
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[storage] store in a ventilated and dry place to prevent moths.

2、 Chemical constituents of Sophora japonica

It mainly contains flavonoids, including:Genistein, genistein-7 - β - d-cellulose diglycoside, genistein-7-diglucosylrhamnoside,Kaempferol, kaempferol-3-o-rhamnosyl diglucoside, kaempferol-3,7-o-diglucoside,quercetin ，rutin，Sophoricoside, Sophora biglycides,Kaempferol-3-o - β - d-sophorolose. also contains triterpenoids andL-lysine，L-aspartic acid ，L-arginine，l-serine , aspartate, glutamate,L-threonine，L-alanine，L-Proline ，L-tryptophan，L-valine ，L-phenylalanine，l-leucine ，L-isoleucineIsoamino acids.
Alkaloids in seeds:Genistein，N-methyl genistein，Sophocarpine，Matrine, ritosamine. It also contains galactose mannan, phospholipids, calcium and magnesium phytate, phytohemagglutinin, etc. The seed oil content is 9.9%. Among the free and bound fatty acids, oleic acid accounts for 22.3%, linoleic acid accounts for 53%, linolenic acid accounts for 12%, andpalmitic acid, stearic acid, octadecenoic acid, octadecadienoic acid, octadecatrienoic acid.

3、 Pharmacological effects of Sophora japonica

1. anti fertility effect: Sophoricoside, genistein and kaempferol contained in Sophora angle have anti fertility effect and estrogen activity in mice.

2. other functions: This product contains a large amount of rutin. Rutin has anti-inflammatory and vitamin P-like effects. It can maintain vascular resistance, reduce its permeability, reduce brittleness and other effects, and has the effect of eliminating fat in the liver with fat infiltration. . The biflavonoid glycosides contained in this product have stronger protective effects on finger peroxidase and antioxidant enzyme than rutin.