1、 Pharmacopoeia standard of Sarcandra glabra
. Harvest in summer and autumn, remove impurities and dry in the sun.
[character] this product is 50 ～ 120cm long. Its rhizome is thick and dense with fine roots. The stem is cylindrical, multi branched, 0.3 ～ 1.3cm in diameter; the surface is dark green to dark brown, with obvious fine longitudinal lines, scattered longitudinal lenticels, and swollen nodes; . The leaves are opposite, ovate lanceolate to ovate elliptic, 5 ～ 15cm long and 3 ～ 6cm wide; the surface is green, greenish brown to brownish brown or brownish red, smooth; The margin is coarse serrated, and the tooth tip gland is dark brown; Petiole is about 1cm long; nearly leathery. Spikes terminal, often branched. The gas is slightly fragrant and the taste is slightly pungent.
[identification] (1) the transverse section of the stem of this product: the epidermal cells are rectangular or oblong, covered with cuticle, and the outer edge is blunt toothed. There are more than 10 rows of cortical cells, 2-3 rows of thick horn cells on the outside, brownish yellow pigment in the inner parenchyma cells, and stone cells scattered in single or groups. The fiber bundles of the columella are crescent shaped, intermittently circular, and lignified. Phloem narrow. Xylem tracheids are numerous, and the rays are 2-8 rows wide. Parenchymal cells in the medulla are larger, and sometimes stone cells can be seen scattered in single or groups.
Powder yellowish green to greenish brown. Wood parenchyma cells are square or rectangular, containing brown yellow pigment. . The fiber is long and narrow fusiform or strip-shaped, with a diameter of 6-30 µ m, wall thickness and lignification. The epidermal cells on the leaves are square or rectangular, the vertical walls are microwave curved or slightly straight, and are covered with thick cuticle. The epidermal cells under the leaves are polygonal, the vertical walls are microwave curved or slightly straight, the stomata are slightly depressed, and there are 3-5 accessory guard cells. Reticulated conduit, threaded conduit and annular conduit are easy to see and non lignified.
(2) Take 2G of the product powder, add 50ml of water, sonicate for 30 minutes, filter, add ethyl acetate to the filtrate, shake and extract twice, 25ml each time, combine the ethyl acetate solution, evaporate to dryness, add 1ml of methanol to dissolve the residue, and use it as the test solution. Another 2G of Sarcandra glabra reference medicinal material was prepared into the reference medicinal material solution by the same method. Then take the isoazinopyridine reference substance and add methanol to make a solution containing 0.5mg per 1ml as the reference substance solution. According to the test of thin-layer chromatography (general rule 0502), suck 4 µ l of each of the above three solutions, dot them on the same silica gel G thin-layer plate, use toluene ethyl acetate formic acid (9:4:1) as the developing agent, develop, take out, dry, and view under the ultraviolet light (365nm). In the chromatogram of the test sample, fluorescent spots of the same color are displayed at the positions corresponding to the chromatogram of the control medicinal material and the chromatogram of the control sample; Fumigate in ammonia vapor for 10 minutes, and the spots corresponding to the chromatogram of the reference substance turn yellow green.
.
The total ash content shall not exceed 10.0% (general rule 2302).
.
[extract] according to the hot leaching method under the determination method of water-soluble extract (general rule 2201), it shall not be less than 10.0%.
. Determine according to high-performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-0.1% phosphoric acid solution (20:80); The detection wavelength was 342nm. The number of theoretical plates should not be less than 4000 according to the peak of isoazine pyridine.
Preparation of reference solution take an appropriate amount of isoazinopyridine reference and rosmarinic acid reference, weigh accurately, and add methanol to make a solution containing 10 µ g per 1ml.
Preparation of test solution take about 0.4g of powder (passing through No. 3 screen), weigh accurately, place in a corked conical flask, add 25ml of methanol precisely, close the stopper, weigh, heat and reflux for 1 hour, cool, weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate.
The determination method is to precisely suck 10 µ l of the reference solution and 10 µ l of the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of isoazine picoline (c11h10o5) shall not be less than 0.020%, and the content of rosmarinic acid (c18h16o8) shall not be less than 0.020%.
Decoction pieces
.
This product has irregular sections. Rhizomes are densely packed with fine roots. . The cut surface is medullary or hollow. The leaves are mostly broken, and the surface is green, greenish brown to brownish brown or brownish red, smooth; The margin is coarse serrated, and the tooth tip gland is dark brown, nearly leathery. The gas is slightly fragrant and the taste is slightly pungent.
[identification] (except for the transverse section) [inspection] [extract] [content determination] is the same as that of medicinal materials.
[nature, taste and meridian tropism] bitter, pungent and peaceful. Heart returning and liver meridian.
[functions and indications] it can clear away heat and cool blood, promote blood circulation and eliminate spots, dispel wind and unblock collaterals. .
.
[storage] store in a ventilated and dry place.

2、 Chemical constituents of Sarcandra glabra

,Isoazinopyridine，Fumarate（fumaric acid）Succinic acid（succinicacid）， Flavonoid glycosides and coumarin derivatives. In addition, it also contains 0.15% - 0.20% volatile oil.

3、

1. animal experiments show that Sarcandra glabra can improve the energy metabolism of tumor cells and tumor bearing mice, increase the activity of catalase, and directly inhibit the oxygen consumption ability of cancer cells and tumor bearing mice. Therefore, the main role of Sarcandra glabra is to improve energy metabolism and achieve anticancer effect. The semi in vivo method proved that the drug could prevent the growth and reproduction of tumor cells in mice after contacting with tumor cells for 2h in vitro. High dose Sarcandra glabra has certain immunosuppressive effects on macrophage system, T lymphocytes and B lymphocytes. Low dose can enhance immune function and regulate immune function.

2. it has antibacterial, antitumor, fracture healing promoting and antiviral effects; The extract and its total flavonoids can promote the phagocytosis of animals; ; Promote appetite.