1、 Pharmacopoeia standard of dog spine
. Excavate in autumn and winter to remove sediment and dry; Or remove the hard roots, petioles and golden villi, cut thick pieces, and dry them to "raw dog ridge pieces"; After steaming, sun it to 60% or 70% dryness, cut it into thick pieces, and dry it. It is "cooked dog spine piece".
[properties] this product is irregular long block, 10 ~ 30cm long, 2 ~ 10cm in diameter. The surface is dark brown, with residual golden villi; There are several reddish brown woody petioles on the top, and black fine roots remain below. It is hard and not easy to break. Odorless, light and slightly astringent. The raw dog ridge piece is irregular long strip or round, 5-20cm long, 2-10cm in diameter, and 1.5-5mm thick; the cut surface is light brown, relatively smooth, and there is a brownish yellow bulge xylem ring or stripe at 1-4mm near the edge, with irregular edges and occasional golden yellow villus residue; It is brittle, easily broken and powdery. Cooked dog fillet is black brown and hard.
. There are more than 10 rows of brown yellow thick walled cells with obvious mural pores. The xylem is arranged in a ring and consists of tracheids, with phloem and endodermis inside and outside. Both cortex and medulla are composed of parenchymal cells, which are filled with starch granules, and some contain yellowish brown substances.
(2) Take 2G of this product powder, add 50ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate, add 1ml of methanol to dissolve the residue, and use it as the test solution. In addition, 2G of the control medicinal material of Rhizoma cibotii was taken, and the control medicinal material solution was prepared by the same method. According to the test of thin-layer chromatography (general rule 0502), suck 3-6 µ l of the test solution and 4 µ l of the control medicinal material solution, respectively dot them on the same silica gel G thin-layer plate, make them into strips, use toluene trichloromethane ethyl acetate formic acid (3:5:6:1) as the developing agent, develop, take out, dry, spray with ferric chloride solution -1% potassium ferricyanide solution (1:1) (prepared temporarily), and place them until the spots are clear. In the chromatogram of the test sample, spots of the same color appear on the corresponding position of the chromatogram of the control medicinal material.
[inspection] the moisture content shall not exceed 13.0% (the second method of general rule 0832).
The total ash content shall not exceed 3.0% (general rule 2302).
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Decoction pieces
[processing] dog ridge to remove impurities; If not sliced, wash, moisten thoroughly, cut into thick pieces, and dry.
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Scald dog's spine take raw dog's spine pieces, scald them with sand according to the scalding method (general rule 0213) until they bulge, and remove the remaining fluff after cooling.
This product is shaped like a dog's back tablet, and its surface is slightly bulging. Tan. It is light and slightly astringent.
[identification] take 2G of this product powder, add 50ml of methanol, sonicate for 30 minutes, filter, evaporate the filtrate, add 1ml of methanol to dissolve the residue as the test solution. In addition, take protocatechuic aldehyde reference substance and protocatechuic acid reference substance, add methanol to make a mixed solution containing 0.5mg per 1ml as the reference solution. According to the test of thin-layer chromatography (general rule 0502), suck 3-6 µ l of the test solution and 2 µ l of the control solution, respectively dot them on the same silica gel G thin-layer plate, make them into strips, use trichloromethane ethyl acetate methanol formic acid (12:2:1:0.8) as the developing agent, develop, take out, dry, spray 2% ferric chloride solution -1% potassium ferricyanide solution (1:1) (prepared for clinical use). In the chromatogram of the test sample, spots with the same color appear at the corresponding position of the chromatogram of the control sample.
[content determination] determine according to high performance liquid chromatography (general rule 0512).
Chromatographic conditions and system suitability test octadecylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-1% glacial acetic acid solution (5:95); The detection wavelength is 260nm. The number of theoretical plates should not be less than 3000 according to the protocatechuic acid peak.
Preparation of reference solution take an appropriate amount of protocatechuic acid reference, weigh accurately, add methanol-1% glacial acetic acid solution (70:30) to make a solution containing 50 µ g per 1ml.
Preparation of test solution take about 1g of powder (passing through No. 3 screen), weigh accurately, place in a corked conical flask, add 25ml of methanol-1% glacial acetic acid solution (70:30) mixed solution precisely, weigh, sonicate (power 250W, frequency 40KHz) for 30 minutes, cool, weigh again, use methanol-1% glacial acetic acid solution (70:30) mixed solution to make up the lost weight, shake well, filter, and take the filtrate.
The determination method is to precisely suck 10 µ l of the reference solution and 10 µ l of the test solution, inject them into the liquid chromatograph, and determine.
According to the calculation of dry product, the content of protocatechuic acid (c7h6o4) shall not be less than 0.020%.
[inspection] [extract] is the same as that of medicinal materials.
[nature, taste and meridian tropism] bitter, sweet and warm. Return to liver and kidney channels.
[functions and indications] dispel wind dampness, tonify liver and kidney, and strengthen waist and knee. It is used for rheumatic arthralgia, waist and knee soreness, and lower limb weakness.
[usage and setting] 6 ～ 12g.
[storage] store in a ventilated and dry place to prevent moisture.

2、 Chemical constituents of civet rhizome

It contains pterosin R, onitin, onitin-2 '- O - β - D-glucoside, onitin-2' - O - β - d-alloside, ptaquiloside, and fern Z.

3、

1. effects on myocardium:
. If 20g/kg was administered continuously for 14 days, the myocardial uptake rate of 86Rb could be increased by 54%.

2. hemostatic effect:
Golden dog hair has a good hemostatic effect on the injury and bleeding of liver, spleen and scar tissue of experimental animals, especially the hemostatic effect of wool powder is the best.