Albizzia bark
1、 Pharmacopoeia standard of Albizzia julibrissin       This product is the dried bark of Albizia juliissin durazz., a leguminous plant. Peel in summer and autumn and dry in the sun.
      [properties] this product is curly cylindrical or semi cylindrical, 40 ～ 80cm long and 0.1 ～ 0.3cm thick. The outer surface is grayish brown to grayish brown, slightly longitudinal wrinkles, some shallow cracks, dense oval transverse lenticels, brown or brownish red, occasionally with raised transverse edges or large round branch marks, often with lichen spots; The inner surface is yellowish brown or yellowish white, smooth, and has fine longitudinal lines.
      It is hard and brittle, easy to break, and the cross section is fibrous, yellowish brown or yellowish white. The breath is slightly fragrant, the taste is light, slightly astringent, slightly irritating, and then there is discomfort in the throat.
      [identification] (1) the powder of this product is grayish yellow. . The fibers are slender, with a diameter of 7-22 µ m, and often in bundles. The surrounding cells contain calcium oxalate crystals, forming crystal fibers. The walls of crystal containing cells are unevenly thickened, lignified or slightly lignified. The diameter of calcium oxalate square crystal is 5 ～ 26 µ M.
      (2) Take 1g of the product powder, add 10ml of 50% methanol to soak for 1 hour, sonicate for 30 minutes, filter, evaporate the filtrate, add 5ml of water to dissolve the residue, shake and extract twice with 5ml of n-butanol, combine the n-butanol solution, evaporate to dryness, add 0.5ml of methanol to dissolve the residue, and use it as the test solution. Another 1g of Albizzia julibrissin skin was used as the control drug solution in the same way. According to the test of thin-layer chromatography (general rule 0502), suck 3 µ l of each of the above two solutions, dot them on the same high-performance silica gel G thin-layer plate, use the lower layer solution of chloroform methanol water (13:5:2) (add 0.1ml of formic acid every 10ml) as the developing agent, develop, take out, dry, spray 5% phosphomolybdic acid ethanol test solution, heat at 90 ℃ until the spots are clear. .
      [check] moisture   Not more than 10.0% (the second method of general rule 0832).
      Total ash   Not more than 6.0% (general rule 2302).
      [extract] according to the hot leaching method under the alcohol soluble extract determination method (general rule 2201), using dilute ethanol as solvent, it shall not be less than 12.0%.
      [content determination] determine according to HPLC (general rule 0512).
      Chromatographic conditions and system suitability test   Octadecylsilane bonded silica gel was used as filler; The mobile phase was acetonitrile-0.04% phosphoric acid solution (18:82); The detection wavelength was 204nm. The number of theoretical plates should not be less than 3000 according to the peak of (-) - syringol-4-o - β - d-carvacosyl - (1 → 2) - β - d-glucopyranoside.
      Preparation of reference solution   Take an appropriate amount of (-) - syringol-4-o - β - d-carvacosyl - (1 → 2) - β - d-glucopyranoside reference substance, accurately weigh it, add methanol to make a solution containing 25 µ g per 1ml.
      Preparation of test solution   Take about 0.5g of this product powder (passing through No. 3 screen), weigh it accurately, place it in a corked conical flask, add 20ml of 50% methanol precisely, close the stopper, weigh it, soak it for 1 hour, sonicate it (power 250W, frequency 40KHz) for 30 minutes, cool it, weigh it again, make up the lost weight with 50% methanol, shake it well, filter it, and take the filtrate.
      Assay   .
      According to the calculation of dried products, the content of (-) - syringinophenol-4-o - β - d-carvacosyl - (1 → 2) - β -d glucopyranoside (c33h44o17) shall not be less than 0.030%.
      Decoction pieces
      [processing] remove impurities, wash, moisten, shred or block, and dry.
      This product is in the form of curved silk or pieces. The outer surface is grayish brown to grayish brown, slightly longitudinally wrinkled, densely covered with obvious oval transverse lenticels, brown or brownish red. The inner surface is yellowish brown or yellowish white, smooth, with fine longitudinal lines. The cut surface is fibrous, yellowish brown or yellowish white. The breath is slightly fragrant, the taste is light, slightly astringent, slightly irritating, and then there is discomfort in the throat.
      .
      [identification], [inspection], [content determination] are the same as those of medicinal materials.
      [nature, taste and meridian tropism] sweet and smooth. Return to heart, liver and lung meridians.
      [functions and indications] relieve depression and calm nerves, promote blood circulation and reduce swelling. It is used for restlessness, melancholy and insomnia, lung carbuncle, sore swelling, falling pain.
      [usage and dosage] 6 ～ 12g. Apply appropriate amount externally, and adjust at the end of research.
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2、 Chemical constituents of Albizzia julibrissin bark
The lignan glucosides in the dried bark of Albizzia julibrissin are: l-syringaresinol-4-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside [syringaresinol-4-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside], l-syringaresinol-4-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranosyl-4 ˊ - O - β - d-glucopyranoside [syringaresinol-4-o - β -d-apiofuranosyl- (1 → 2) - β - d-glucopyranosyl-4 ˊ -o- β - d-glucopyranoside], l-syringol-4, 4 ˊ - bis-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside [syringaresinol-4, 4 ˊ - bis-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside], l-syringaresinol-4-o - β - d-glucopyranoside, l-syringaresinol-4, 4 ˊ - bis-o - β - d-glucopyranoside,aresinol-4, 4 ˊ - bis-o - β - d-glucopyranoside), which also contains methyl syringate-4-o - β - d-apifurosyl - (1 → 2) - β - d-glucopyranoside [syringic Acid methyl ester-4-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside], glaberide-i-4-o - β - d-glucopyranoside, glaberide-a-4-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside [glaberide i-4-o - β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside]-glucopyranoside], dextro-5,5 ˊ - dimethoxylarinol-4-o - β - d-apifuranosyl - (1 → 2) - β - d-glucopyranoside [5, 5 ˊ -dimethoxylariciresinol-4-o- β -d-apiofuranosyl- (1 → 2) - β -d-glucopyranoside] and 5,5 ˊ - dimethoxy-7-oxoflagerol-4 ˊ -o- β - d-apiofuranosyl - (1 → 2) - β - d-glucopyranoside [5,5 ˊ -dimethoxy-7-oxolariciresinol-4 ˊ -o- β -d-apiofuranosyl- (1 → 2) - β - d-glucopyranoside]anoside]. In addition, the dried skin also contains 21-[4- (ethylidene) - 2-tetrahydrofuran isobutyryl] folinic acid

3、 Pharmacological effects of Albizzia julibrissin bark
1. antifertility effect the cold water extract of Albizzia julibrissin bark has significant antifertility effect. Intraamniotic administration can make the fetus of mid pregnancy rats atrophy and pale, and terminate pregnancy. Under the action of Albizzia julibrissin extract, the uterine muscle strips of human pregnancy contract, and the tension and amplitude are significantly increased, while the contraction frequency is significantly reduced. The effect of Albizzia julibrissin is similar to oxytocin, but the onset time is slow and the duration is long. The effective antifertility component of Albizzia julibrissin skin is saponin. Subcutaneous injection of 1.78mg/kg total saponins of Albizzia julibrissin skin has significant anti implantation effect, can reduce the number of pregnant animals and normal embryos in rats, and the pregnancy termination rate is 86%. Administration on the 4th-6th day of pregnancy also had significant anti early pregnancy effect, and the pregnancy termination rate was 40%. . Intraperitoneal injection of 70 μ g/kg (1 / 50 of the LD50 of mice) once a day for a total of 3D could not increase the weight of uterus of young female mice, indicating that it had no estrogen like activity.
. 2. antiallergic Albizzia cortex decoction can inhibit the degranulation of peritoneal mast cells in rats by gavage, and it also has a similar effect in vitro. Albizzia cortex decoction can significantly inhibit the sensitization process and antibody production process of antigen (horse serum) in rats. Its active ingredient seems to be a heat-resistant water-soluble substance.
3. anti tumor effect the inhibitory rate of polysaccharide contained in Albizzia julibrissin bark on transplanted tumor S180 in mice was 73%.