Vason
1、 Vassone pharmacopoeia standard       This product is the dry aboveground part of the Crassulaceae plant orostachys fimbriata (turcz.) berg. Harvest the flowers in summer and autumn, remove roots and impurities, and dry them in the sun.
      [character] the stem of this product is slender cylindrical, 5-27cm long, 2-6mm in diameter. The surface is grayish brown, with many protruding residual leaf bases and obvious longitudinal edges. The leaves are mostly detached, broken or curly, gray green. The panicle is spicate, the floret is white or pink, and the pedicel is about 5mm long. It is light, brittle, and fragile. It is slightly smelly and tastes sour.
      [identification] (1) cross section of the stem of this product: the outermost layer is a row of epidermal cells, rectangular or nearly square, covered with cuticle. . The vascular bundle is tough outside, the cambium forms a ring, and the xylem vessels are arranged neatly. The central peduncle is large, and the parenchyma cells often contain reddish brown substances.
      (2) Take 5g of this product powder, add 50ml of methanol-25% hydrochloric acid solution (4:1) mixed solution, heat and reflux for 1 hour, filter, evaporate the filtrate to near dryness, add 20ml of water to dissolve the residue, shake and extract twice with ethyl acetate, 20ml each time, combine the ethyl acetate solution, wash with 10ml of water, discard the water solution, volatilize the filtrate, add 2ml of methanol to dissolve the residue, and use it as the test solution. Another 2G of vassone reference drug was taken and prepared into the reference drug solution by the same method. Then take kaempferol reference substance and add methanol to make a solution containing 0.5mg per 1ml as the reference substance solution. According to the test of thin-layer chromatography (general rule 0502), suck 5 μ l each of the test solution and the control medicinal material solution, and 2 μ l of the control solution, respectively, and dot them on the same silica gel G thin-layer plate prepared with 1% sodium hydroxide solution, develop them with toluene ethyl acetate formic acid (25:20:1), take them out, dry them, spray 10% aluminum trichloride ethanol solution, and inspect them under the UV light (365nm). In the test sample chromatography, fluorescent spots of the same color appear at the corresponding positions of the control medicinal material chromatography and the control sample chromatography.
      ; Not more than 2% (general rule 2301).
      Moisture   Not more than 13.0% (the second method of general rule 0832).
      [extract] according to the hot leaching method under the determination method of alcohol soluble extract (general rule 2201), the use of ethanol as solvent shall not be less than 3.0%.
      [content determination] determine according to HPLC (general rule 0512).
      ; Octadecylsilane bonded silica gel was used as filler; The mobile phase was methanol-0.5% phosphoric acid solution (47:53); The detection wavelength is 360nm. The number of theoretical plates should not be less than 4000 according to the quercetin peak.
      Preparation of reference solution   Take an appropriate amount of quercetin reference substance and kaempferol reference substance, weigh accurately, add methanol to make a mixed solution containing 10 μ g quercetin and 20 μ g kaempferol per 1ml.
      Preparation of test solution   Take about 1g of this product powder (through No. 3 screen), weigh it accurately, place it in a corked conical flask, add 50ml of methanol-25% hydrochloric acid solution (4:1) mixture solution precisely, close the stopper, weigh it, put it in a water bath and reflux for 1 hour, cool it immediately, weigh it again, make up the weight lost with methanol, shake it well, filter it, and take the filtrate.
      ; Precisely suck 10 μ l of the reference solution and 10 ～ 20 μ l of the test solution respectively, inject them into the liquid chromatograph, and determine,
      The total amount of quercetin (C15H10O7) and kaempferol (c15h10o6) in this product shall not be less than 0.020% according to the dry product.
      Decoction pieces
      [processing] remove residual roots and impurities and cut into sections.
      [identification], [inspection], (moisture), [extract], [content determination] are the same as those of medicinal materials.
      [nature, taste and meridian tropism] sour, bitter and cool. It belongs to the liver, lung and spleen meridians.
      [functions and indications] cool blood, stop bleeding, detoxify, and heal sores. It is used for blood dysentery, hematochezia, hemorrhoids and wounds that do not heal for a long time.
      [usage and dosage] 3 ～ 9g. Apply appropriate amount externally, and coat the affected part with grinding powder.
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2、 Chemical constituents of Pinus armandi
Pinus armandi whole grass containsquercetin （quercetin）， Quercetin-3-glucoside,Kaempferol（kaempferol），Kaempferol-7-o-glucoside（Kaempferol-7-O-beta-D-glucopyranoside），Quercetin-3-o-glucose-7-o-rhamnoside(quercetin 3-o-glucoside-7-o-rhamnoside) and oxalic acid. Evening ormosia contains oxalic acid.

3、
Anesthetized dogs and rabbits were given intravenous injection of vaccum Flos extract. Blood pressure first increased and then decreased, but soon recovered. It is slightly excited about breathing, and can strengthen contraction and reduce heart rate to isolated toad heart. Vascular perfusion of isolated toad's lower limbs can make it contract, and it has obvious excitatory effect on isolated rabbit intestine. .
1. cardiotonic effect: the 100% concentration of the whole herb decoction 0.7ml/kg is intravenously injected to enhance the cardiac contraction of anesthetized rabbits. The intravenous drip of 50% Decoction 0.5ml/kg slows the heart rate of guinea pigs, moves down the ST segment, and flattens or inverts the T wave. The biological titer measured by pigeon method is 0.23 digitalis units per gram of dry plants.
2. anti inflammation and analgesia: vassone crystal I (3b-c1) 300mg/kg intraperitoneal injection can inhibit the ear inflammation in mice caused by dimethylbenzine and the paw swelling in rats caused by carrageenan; 200mg/kg intraperitoneal injection could inhibit the granuloma induced by croton oil in rats; 300mg/kg intraperitoneal injection could inhibit the writhing reaction induced by acetic acid in mice. .